本文選題：載脂蛋白A + 多態(tài)性　； 參考：《中山大學(xué)學(xué)報(醫(yī)學(xué)科學(xué)版)》2017年05期

【摘要】：【目的】探討中國漢族人群載脂蛋白A5(APOA5)基因rs2075291和rs3135507多態(tài)性位點與冠心病(CHD)患者血脂水平及冠脈狹窄程度的關(guān)系�！痉椒ā繎�(yīng)用聚合酶鏈?zhǔn)椒磻?yīng)-限制性片段長度多態(tài)性法(PCR-RFLP)對324例CHD患者和152例非CHD對照者進(jìn)行rs2075291和rs3135507多態(tài)性分型并比較不同基因型之間血脂水平的差異。采用Gensini評分系統(tǒng)評價CHD患者的冠脈狹窄程度,并使用多因素線性回歸法分析rs2075291和rs3135507多態(tài)性以及其他因素與Gensini得分之間的相關(guān)性�！窘Y(jié)果】CHD組高血壓患病率、甘油三酯(TG)、總膽固醇(TC)、載脂蛋白B100(APOB100)、脂蛋白a[Lp(a)]、TG/高密度脂蛋白膽固醇(HDL-C)、TC/HDL-C、低密度脂蛋白膽固醇(LDL-C)/HDL-C和APOB100/載脂蛋白AI(APOAI)水平高于對照組,HDL-C和APOAI水平低于對照組,差異均有統(tǒng)計學(xué)意義(P0.05)。rs2075291和rs3135507多態(tài)性等位基因及基因型分布頻率在CHD組與對照組之間差異無統(tǒng)計學(xué)意義。在對照組中,rs2075291多態(tài)性GT基因型TG和TG/HDL-C水平高于GG基因型,HDL-C水平低于GG基因型,差異均有統(tǒng)計學(xué)意義(P0.05);在CHD組中,rs2075291多態(tài)性GT基因型TG/HDL-C和TC/HDL-C高于GG基因型,差異有統(tǒng)計學(xué)意義(P0.05)。在CHD組中,rs3135507位點A等位基因攜帶者APOB100水平低于GG基因型,差異有統(tǒng)計學(xué)意義(P0.05)。多因素回歸分析結(jié)果顯示,rs2075291和rs3135507多態(tài)性與Gensini得分的相關(guān)性無統(tǒng)計學(xué)意義(P0.05)。【結(jié)論】APOA5 rs2075291影響血脂水平,但與CHD發(fā)生發(fā)展無明顯相關(guān)性,此研究結(jié)果需大樣本多中心的病例-對照研究加以證實。
[Abstract]:[objective] to investigate the relationship between rs2075291 and rs3135507 polymorphism of apolipoprotein A5apOA5 gene and the level of blood lipid and the degree of coronary stenosis in patients with coronary heart disease (CHD). [methods] Polymerase chain reaction-restriction fragment length was used. The polymorphism of rs2075291 and rs3135507 were detected by PCR-RFLP in 324 CHD patients and 152 non-CHD controls, and the differences of blood lipid levels among different genotypes were compared. The degree of coronary artery stenosis in patients with CHD was evaluated by Gensini scoring system, and the correlation between rs2075291, rs3135507 polymorphism and other factors and Gensini score was analyzed by multivariate linear regression. [results] the prevalence of hypertension in CHD group was analyzed. The levels of TG / HDL-C, LDL-CU HDL-C and APOB100/ apolipoprotein AIAPOAI in the control group were higher than those in the control group, and the levels of HDL-C and APOAI in the control group were significantly higher than those in the control group, and the levels of HDL-C and APOAI in the control group were lower than those in the control group, and the levels of HDL-C / HDL-C were higher than those in the control group, and the levels of HDL-C and APOAI in the control group were significantly higher than those in the control group, and the levels of HDL-C / HDL-C were higher than those in the control group. There was no significant difference in alleles and genotypes between CHD group and control group. In the control group, the TG and TG/HDL-C levels of rs2075291 polymorphism GT genotype were higher than those of GG genotype and HDL-C level was lower than that of GG genotype, the difference was statistically significant (P 0.05), while the TG/HDL-C and TC/HDL-C levels of rs2075291 polymorphism GT genotype in CHD group were higher than those of GG genotype (P 0.05). In CHD group, the APOB100 level of allele A at Rs3135507 was lower than that of GG genotype, and the difference was statistically significant (P 0.05). Multivariate regression analysis showed that rs2075291 and rs3135507 polymorphism had no significant correlation with Gensini score. [conclusion] APOA5 rs2075291 affects blood lipid level, but has no significant correlation with the occurrence and development of CHD. This study needs to be confirmed by a large-sample multi-center case-control study.
【作者單位】： 川北醫(yī)學(xué)院附屬醫(yī)院心內(nèi)科;雅安職業(yè)技術(shù)學(xué)院醫(yī)學(xué)系;川北醫(yī)學(xué)院藥學(xué)院藥物研究所;川北醫(yī)學(xué)院臨床醫(yī)學(xué)系;川北醫(yī)學(xué)院醫(yī)學(xué)檢驗系;川北醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院生化教研室;
【基金】：四川省教育廳重點項目(17ZA0172) 川北醫(yī)學(xué)院科研創(chuàng)新團(tuán)隊項目(CBY13-TD02)
【分類號】：R541.4

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