本文選題：失血 + 心臟驟停�。� 參考：《廣東醫(yī)學(xué)》2017年08期

【摘要】：目的構(gòu)建失血性心臟驟停兔模型,以滿足失血性心臟驟�；A(chǔ)研究的需要。方法選擇27只新西蘭兔分為假手術(shù)組(A組,無放血、無復(fù)蘇)、未控制組(B組,放血但無復(fù)蘇)、液體復(fù)蘇組(C組,放血及復(fù)蘇)。A組不作處理,B、C組經(jīng)動靜脈放血約放出50%的全身血量,達(dá)到心臟驟停后維持狀態(tài)15 min,隨后采用乳酸林格液和全血回輸復(fù)蘇。各時相點(diǎn)采動脈血測血?dú)夥治霾⒔y(tǒng)計輸液量、失血量及6 h存活率。結(jié)果建模成功時B、C組血壓顯著降低,C組平均動脈壓(MAP)下降至(14.5±5.3)mmHg。30 min MAP為(69.75±14.37)mmHg。在復(fù)蘇5 min時pH值、動脈二氧化碳分壓(PaCO_2)、紅細(xì)胞壓積(Hct)、血紅蛋白(Hb)、實際碳酸氫鹽(AB)、標(biāo)準(zhǔn)碳酸氫鹽(SB)、實際堿剩余(ABE)、標(biāo)準(zhǔn)堿剩余(SBE)顯著降低,K~+、乳酸水平顯著升高(與0 min比較:P0.01);在復(fù)蘇25 min,僅K~+恢復(fù)至0 min水平,而AB、SB、SBE、ABE與乳酸均低于0 min水平(P0.05)。Ca~(2+)在所測時間點(diǎn)皆與T0時間點(diǎn)比較差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論通過動靜脈聯(lián)合放血構(gòu)建的模型穩(wěn)定性和可重復(fù)性高,適用于失血性心臟驟停的研究。
[Abstract]:Objective to establish a rabbit model of hemorrhagic cardiac arrest to meet the needs of basic research on hemorrhagic cardiac arrest. Methods Twenty-seven New Zealand rabbits were divided into two groups: sham operation group (group A), no bleeding, no resuscitation, no control group (group B), bleeding without resuscitation and fluid resuscitation group (group C). About 50% of the whole body blood volume was released through arteriovenous bleeding in group A and resuscitation group A, which was maintained for 15 min after cardiac arrest, and then was resuscitated with Ringer lactate solution and whole blood transfusion. Arterial blood was collected at each time point for blood gas analysis and blood transfusion volume, blood loss and 6 h survival rate were counted. Results when the model was successfully established, the mean arterial pressure in BPC group was significantly decreased to 14.5 鹵5.3)mmHg.30 min MAP (69.75 鹵14.37mmHg). At 5 min after resuscitation, pH value, arterial partial pressure of carbon dioxide (Paco _ 2), hematocrit (HCT), hemoglobin (HB), actual bicarbonate (ABN), standard bicarbonate (SBE), actual base residue (ABE), standard base residual (SBE) decreased significantly. The level of lactic acid increased significantly (compared with 0 min, only K0 min was recovered to 0 min level at 25 min after resuscitation, but both ABN SBE and lactic acid were lower than 0 min level P0.05 路CaP0 2). There was no significant difference between the measured time points and T0 time points (P 0.05). Conclusion the model constructed by combined arteriovenous bleeding has high stability and reproducibility and is suitable for the study of hemorrhagic cardiac arrest.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院急診科;
【基金】：廣東省科技計劃項目(編號:2013B010401014,2014A020212192)
【分類號】：R-332;R541.78

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