發(fā)布時間：2018-04-24 11:55

  本文選題：心絞痛 + 不穩(wěn)定型��； 參考：《解放軍醫(yī)學(xué)雜志》2017年06期

【摘要】：目的分析冠心病不穩(wěn)定型心絞痛(UAP)與穩(wěn)定型心絞痛(SAP)患者血漿中的差異蛋白質(zhì),篩選可能與UAP早期診斷密切相關(guān)的血漿蛋白標(biāo)記物。方法分別收集2014年6月-2015年4月南方醫(yī)科大學(xué)第三附屬醫(yī)院UAP及SAP血漿標(biāo)本各60例,另收集體檢科收集的血漿標(biāo)本作為正常對照組(n=60)。隨機取對照組(n=10)、UAP組(n=10)與SAP組(n=10)空腹血漿標(biāo)本各100μl,分別等量混合成3組樣本,去除血漿高豐度蛋白后,利用雙向差異凝膠電泳(DIGE)技術(shù)進行蛋白分離,經(jīng)差異軟件分析后,采集UAP和SAP之間變化2倍以上的差異蛋白質(zhì)點,利用基質(zhì)輔助激光解吸電離-飛行時間/飛行時間質(zhì)譜(MALDI-TOF/TOF MS)對差異蛋白點進行鑒定。每組隨機選取40份血漿樣本,選取UAP特異性差異蛋白進行ELISA驗證。結(jié)果 UAP與SAP組患者血漿相比較,共篩選出10個表達量差異2倍以上的差異蛋白點,包括9個上調(diào)蛋白點,1個下調(diào)蛋白點。經(jīng)質(zhì)譜鑒定后,表達上調(diào)的蛋白包括纖維蛋白原γ鏈(FGG)、補體C4-B(C4B)、免疫球蛋白κ鏈C結(jié)構(gòu)域(IGKC)和血紅蛋白α亞基(HBA1);表達下調(diào)的蛋白是結(jié)合珠蛋白(HP)。與對照組比較后,在這些差異蛋白中共找到2個UAP特異性相關(guān)蛋白,即IGKC和HP。選取IGKC進行ELISA驗證,結(jié)果表明,與對照組和SAP組相比較,UAP組樣本中IGKC特異性表達上調(diào)(P0.05),與DIGE驗證結(jié)果一致。結(jié)論篩選到UAP特異性相關(guān)蛋白IGKC和HP,IGKC有可能成為UAP早期篩查及診斷的特異性生物標(biāo)記物。
[Abstract]:Objective to analyze the differential proteins in the plasma of patients with unstable angina pectoris (UAP) and stable angina pectoris (SAP) and to screen the plasma protein markers which may be closely related to the early diagnosis of UAP. Methods the plasma samples of UAP and SAP were collected from June 2014 to April 2015 in the third affiliated Hospital of Southern Medical University, respectively. The plasma samples collected from the Department of physical examination were taken as the normal control group. The fasting plasma samples of the control group and the SAP group were randomly mixed into 3 groups in the same volume. After removing the plasma high abundance protein, the protein was separated by two-dimensional differential gel electrophoresis (DIGE), and the protein was analyzed by differential software. The differential protein spots were collected and identified by matrix assisted laser desorption ionization / time of flight mass spectrometry (MALDI-TOF / TOF MS). 40 plasma samples were randomly selected in each group and UAP specific differential protein was selected for ELISA verification. Results 10 differentially expressed protein spots, including 9 up-regulated protein spots and 1 down-regulated protein point, were screened out in UAP group compared with those in SAP group. The up-regulated proteins were identified by mass spectrometry, including fibrinogen 緯 chain FGGGG, complement C4-BnC4BN, immunoglobulin 魏 chain C domain IGKC) and hemoglobin 偽 subunit HBA1C, and the down-regulated protein was the binding globin (HPN). Compared with the control group, two UAP specific related proteins, IGKC and IGKC, were found in these differentially expressed proteins. IGKC was selected for ELISA validation. The results showed that the specific expression of IGKC was up-regulated in UAP group compared with control group and SAP group, which was consistent with the result of DIGE verification. Conclusion the screening of UAP specific related proteins IGKC and HPG-IGKC may be specific biomarkers for early screening and diagnosis of UAP.
【作者單位】： 南方醫(yī)科大學(xué)病理生理學(xué)教研室、廣東省蛋白質(zhì)組學(xué)重點實驗室;南方醫(yī)科大學(xué)第三附屬醫(yī)院心血管內(nèi)科;南方醫(yī)科大學(xué)第三附屬醫(yī)院檢驗科;
【基金】：廣東省科技計劃項目(2013B021800309)~~
【分類號】：R541.4

【參考文獻】

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本文編號：1796518