發(fā)布時(shí)間：2018-02-26 18:46

  本文關(guān)鍵詞： 等長收縮 心肌缺血 側(cè)支動(dòng)脈生成 單核細(xì)胞 平滑肌細(xì)胞　出處：《中國康復(fù)醫(yī)學(xué)雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的:觀察等長收縮訓(xùn)練對(duì)犬慢性缺血心肌側(cè)支動(dòng)脈生成的影響。方法:應(yīng)用Ameroid縮窄器制備犬慢性冠狀動(dòng)脈狹窄模型,造模成功后,將模型動(dòng)物隨機(jī)分為3組:假手術(shù)組(SO,n=6),單純心肌缺血組(OMI,n=6),被動(dòng)等長收縮訓(xùn)練組(PIE,n=6)。SO組進(jìn)行開胸手術(shù),不進(jìn)行慢性心肌缺血造模,也不給予任何缺血刺激和運(yùn)動(dòng)訓(xùn)練,安靜籠養(yǎng);OMI組手術(shù)進(jìn)行慢性心肌缺血造模,不進(jìn)行任何運(yùn)動(dòng)訓(xùn)練;PIE組手術(shù)進(jìn)行慢性心肌缺血造模,關(guān)胸后休息1周后開始被動(dòng)等長收縮訓(xùn)練,采用100%最大強(qiáng)度訓(xùn)練1min,休息1min,每日重復(fù)20次,每周訓(xùn)練5天,訓(xùn)練時(shí)程為6w。實(shí)驗(yàn)終點(diǎn)時(shí),所有模型犬采用心臟SPECT成像測定心肌缺血區(qū)域血流灌注;微球技術(shù)檢測缺血心肌冠狀動(dòng)脈側(cè)支循環(huán)血流量;免疫組化法測定犬缺血心肌組織單核細(xì)胞、內(nèi)皮細(xì)胞及平滑肌細(xì)胞數(shù)量,小動(dòng)脈密度。結(jié)果:6周PIE后,OMI組的靜息血流灌注心肌節(jié)段總評(píng)分最高,PIE組的值高于SO組。冠狀動(dòng)脈側(cè)支循環(huán)血流量(MBF)在PIE組顯著高于OMI組及SO組。PIE組的內(nèi)皮細(xì)胞數(shù)量、單核細(xì)胞數(shù)量、平滑肌細(xì)胞數(shù)量均顯著高于其他各組。通過相關(guān)性分析發(fā)現(xiàn),小動(dòng)脈密度與MBF呈正相關(guān);內(nèi)皮細(xì)胞數(shù)量、平滑肌細(xì)胞數(shù)量、單核細(xì)胞數(shù)量均與小動(dòng)脈密度呈正相關(guān)。結(jié)論:等長收縮訓(xùn)練可以促進(jìn)遠(yuǎn)隔缺血心肌側(cè)支動(dòng)脈生成,改善側(cè)支循環(huán)血流,實(shí)現(xiàn)"生物搭橋"。
[Abstract]:Objective: to observe the effect of isometric contraction training on the formation of collateral arteries in dogs with chronic ischemia. Methods: the canine model of chronic coronary artery stenosis was established with Ameroid constrictor. The model animals were randomly divided into three groups: sham operation group, simple myocardial ischemia group, and passive isometric contraction training group. The model of chronic myocardial ischemia was established in the OMI group without any exercise training. The model of chronic myocardial ischemia was established in the pie group, and the passive isometric contraction training began after one week's rest after chest closure. 100% maximum intensity training was used for 1 minute, rest for 1 minute, repeated 20 times a day for 5 days a week for 6 weeks. At the end of the experiment, all the model dogs were examined by cardiac SPECT imaging. The amount of monocytes, endothelial cells and smooth muscle cells in canine ischemic myocardium was measured by microsphere technique, and the number of monocytes, endothelial cells and smooth muscle cells in canine ischemic myocardium was determined by immunohistochemical method. Results the total score of resting perfusion myocardial segments in the PIE group was higher than that in the so group. The number of endothelial cells in the PIE group was significantly higher than that in the OMI group and the so. Pie group. The number of monocytes and smooth muscle cells were significantly higher than those of other groups. The correlation analysis showed that the density of arterioles was positively correlated with MBF, the number of endothelial cells, the number of smooth muscle cells, the number of smooth muscle cells, The number of monocytes was positively correlated with the density of arterioles. Conclusion: isometric contractile training can promote the formation of collateral arteries, improve the collateral circulation, and realize "biological bypass".
【作者單位】： 復(fù)旦大學(xué)附屬婦產(chǎn)科醫(yī)院;南京醫(yī)科大學(xué)第一附屬醫(yī)院;寧波市李惠利醫(yī)院;南京醫(yī)科大學(xué)附屬南京醫(yī)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81472164)
【分類號(hào)】：R541

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