本文關(guān)鍵詞： 腹主動脈瘤 彈力蛋白酶　出處：《中國老年學(xué)雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的通過經(jīng)腹主動脈腔內(nèi)灌注高濃度彈力蛋白酶建立兔腹主動脈瘤(AAA)模型,觀察不同灌注保持時間對兔AAA成瘤率及死亡率的影響。方法 24只新西蘭雄兔隨機(jī)分為A組、B組、C組各8只,每組均經(jīng)雙腔導(dǎo)管注入10μl、濃度為100 U/ml的彈力蛋白酶,灌注保持時間A組3~5 min,B組7~10 min,C組20~30 min;后用生理鹽水沖洗管腔3次;分別于術(shù)前及術(shù)后第7天行腹主動脈彩超檢查,術(shù)后第7天處死動物,行組織學(xué)病理染色觀察血管灌注部位組織學(xué)變化。結(jié)果 A組存活8只,灌注局部血管擴(kuò)張率40.3%~63.1%,其中5只擴(kuò)張率大于50%,達(dá)到AAA成瘤標(biāo)準(zhǔn),成瘤率62.5%,術(shù)后7 d存活率100%;B組存活8只,局部血管擴(kuò)張率87.5%~109.2%,均達(dá)到成瘤標(biāo)準(zhǔn),成瘤率100%,術(shù)后7 d存活率100%;C組存活3只,灌注局部血管擴(kuò)張率237.7%~252.0%,成瘤率100%,術(shù)后7 d存活率為37.5%。結(jié)論高濃度彈力蛋白酶制作兔AAA模型,灌注時間保持7~10 min具有成瘤率高、死亡率低、操作簡易等優(yōu)點(diǎn),易于推廣。
[Abstract]:Objective to establish an AAA model of abdominal aortic aneurysm in rabbits by intraluminal perfusion of high concentration elastase through abdominal aorta. Methods 24 New Zealand male rabbits were randomly divided into group A (n = 8), group B (n = 8) and group C (n = 8). The perfusion retention time of group A was 3 minutes 5 minutes, group B was 7 minutes 10 minutes and group C was 20 minutes 30 minutes, and then rinsed the lumen with normal saline for 3 times. The abdominal aorta was examined by color Doppler ultrasonography before and 7 days after operation, and the animals were killed on the 7th day after operation. Results in group A, 8 rats survived, and the local vasodilation rate was 40.3%, 63.1%, 5 of them were larger than 50, which reached the standard of AAA tumorigenesis. The tumor formation rate was 62.5%. The survival rate of group B was 100 on the 7th day after operation. The local vasodilation rate was 87.5% and 109.2%, all of them reached the standard of tumorigenesis, and the tumorigenesis rate was 100%. The survival rate of group C was 100% on the 7th day after operation, and 3 rats in group C survived on the 7th day after operation. The local vasodilation rate was 237.7% and the tumorigenesis rate was 100%. The survival rate was 37.50.Conclusion the AAA model of rabbits made by high concentration elastase has the advantages of high tumorigenesis rate, low mortality, easy operation and easy to be popularized.
【作者單位】： 首都醫(yī)科大學(xué)附屬北京安貞醫(yī)院心內(nèi)科;
【基金】：國家自然科學(xué)基金(No.81370437)
【分類號】：R-332;R543.16

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