目的:探索F-box蛋白3(F-box protein 3,FBXO3)抑制劑BC-1215對(duì)三硝基苯磺酸(trinitrobenzene sulfonic acid,TNBS)誘導(dǎo)的小鼠腸黏膜炎癥反應(yīng)的作用。方法:將Balb/c小鼠隨機(jī)分為4組,每組10只,2組給予TNBS灌腸誘導(dǎo)結(jié)腸炎(實(shí)驗(yàn)組),其中1組給予BC-1215腹腔注射,另1組給予磷酸緩沖鹽溶液(PBS)腹腔注射;另外2組給予等體積50%乙醇灌腸作為陰性對(duì)照組,亦分別給予BC-1215或PBS腹腔注射。5d后收集結(jié)腸組織,通過對(duì)比體重、結(jié)腸縮短程度、結(jié)腸組織蘇木精-伊紅染色(hematoxylin-eosin staining,HE)及髓過氧化物酶活性評(píng)估BC-1215對(duì)腸黏膜炎癥影響,使用熒光定量PCR(qRT-PCR)檢測(cè)腸黏膜組織中炎癥細(xì)胞因子IL-6,TNF-α,IL-1β,IL-23及Th1細(xì)胞和Th17細(xì)胞相關(guān)細(xì)胞因子(IFN-γ和IL-17A)和轉(zhuǎn)錄因子(T-bet和RORγt)表達(dá)。結(jié)果:BC-1215抑制TNBS誘導(dǎo)的小鼠體重下降、減輕小鼠結(jié)腸縮短程度和炎癥細(xì)胞浸潤,并且抑制小鼠結(jié)腸組織中炎癥細(xì)胞因子表...

【文章頁數(shù)】：7 頁

【部分圖文】：

圖1BC-1215減輕TNBS誘導(dǎo)的小鼠炎癥程度Figure1BC-1215amelioratedtheseverityofTNBS-inducedcolitisControlTNBSControlTNBS00NN

2244臨床與病理雜志,2020,40(9)http://lcbl.amegroups.com圖1BC-1215減輕TNBS誘導(dǎo)的小鼠炎癥程度Figure1BC-1215amelioratedtheseverityofTNBS-inducedcolitis各組小鼠體重(A)、結(jié)腸....

圖2BC-1215抑制TNBS誘導(dǎo)的炎癥細(xì)胞因子表達(dá)Figure2BC-1215suppressedtheexpressionofinflammatorycyto30IL-6mRControlTNBS50IL-1βmControlTNBS

BC-1215抑制三硝基苯磺酸誘導(dǎo)的小鼠腸黏膜炎癥反應(yīng)石巖，等2245圖2BC-1215抑制TNBS誘導(dǎo)的炎癥細(xì)胞因子表達(dá)Figure2BC-1215suppressedtheexpressionofinflammatorycytokineinTNBS-inducedcoliti....

圖2BC-1215抑制TNBS誘導(dǎo)的炎癥細(xì)胞因子表達(dá)Figure2BC-1215suppressedtheexpressionofinflammatorycyto30IL-6mRControlTNBS50IL-1βmControlTNBS

BC-1215抑制三硝基苯磺酸誘導(dǎo)的小鼠腸黏膜炎癥反應(yīng)石巖，等2245圖2BC-1215抑制TNBS誘導(dǎo)的炎癥細(xì)胞因子表達(dá)Figure2BC-1215suppressedtheexpressionofinflammatorycytokineinTNBS-inducedcoliti....

圖3BC-1215抑制小鼠體內(nèi)Th1和Th17細(xì)胞免疫反應(yīng)Figure3BC-1215inhibitedTh1/Th17-mediatedimmuneresponseinTNBS-inducedcolitismice2001000T-betRORγControlTNBSControlTNBSControlTNBS

BC-1215抑制三硝基苯磺酸誘導(dǎo)的小鼠腸黏膜炎癥反應(yīng)石巖，等2245圖2BC-1215抑制TNBS誘導(dǎo)的炎癥細(xì)胞因子表達(dá)Figure2BC-1215suppressedtheexpressionofinflammatorycytokineinTNBS-inducedcoliti....

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