OBJECTIVE: To investigate the inhibitory effect of Panax notoginseng saponins(PNS) on liver fibrosis and explore the underlying mechanisms.METHODS: Carbon tetrachloride(CCl4)-treated rats and hepatic stellate cells(HSCs) were used. The effect of PNS on CCl4-induced liver fibrosis was studied with histochemical and biochemical analysis.Transforming growth factor(TGF)-β1, α-smooth muscle actin(α-SMA), and collagen Ι m RNA expression were determined by reverse transcriptase-polymerase chain reactio...

【文章頁數(shù)】：8 頁

【文章目錄】：
INTRODUCTION
MATERIALS AND METHODS
    Experimental animals
    Reagents
    Model replication and administration
    Cell culture
    Calculations of liver index and spleen index
    Measurements of serum AST, ALT, and tissue Hyp
    Histopathological changes
    Immunohistochemical examination
    RT-PCR assay
    Western blotting analysis
    Statistical analysis
RESULTS
    PNS ameliorated CCl4-induced liver fibrosis in rats
    Organ indexes
    Immunohistochemistry of α-SMA and p-Jak2
    Effect of PNS on TGF-β1, α-SMA, and collagen I m RNA expression in vitro and in vivo
    Effects of PNS on collagen I, α-SMA, p-Jak2, and p-Stat3 protein expressions in vitro
DISCUSSION



本文編號(hào)：3873920