Objective Interferon-γ（IFN-γ） plays an important role in apoptosis and was shown to increase the risk of diabetes. Visfatin, an adipokine, has anti-diabetic, anti-tumor, and regulating inflammatory properties. In this study we investigated the effect of visfatin on IFN-γ-induced apoptosis in rat pancreatic β-cells. Methods The RINm5F（rat insulinoma cell line） cells exposed to IFN-γ were treated with or without visfatin. The viability and apoptosis of the cells were assessed by using MTT and flow... 

【文章來(lái)源】：Biomedical and Environmental Sciences. 2015,28(03)

【文章頁(yè)數(shù)】：9 頁(yè)

【文章目錄】：
INTRODUCTION
METHODS
    Materials
    Cell Culture
    RNA Extraction and Quantitative Real-time PCR
    1-（4, 5-dimethylthiazol-2-yl）-3, 5-diphenylformazan Assay
    Flow-cytometric Analysis for Apoptosis
    Measurement of Caspase-3 Activity
    Western Blot Analysis
    Statistical Analysis
RESULTS
    Visfatin Protects RINm5F Cells Against IFN-γInduced Apoptosis
    VisfatinInhibitesMitochondria-dependent Apoptotic Pathway
    Visfatin Induces Activation of AMPK and ERK1/2Signaling Pathway
    Anti-apoptoticActionof Visfatin Requires Activation of AMPK and ERK1/2-mediated Signaling Pathways
DISCUSSION



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