Objective Interferon-γ（IFN-γ） plays an important role in apoptosis and was shown to increase the risk of diabetes. Visfatin, an adipokine, has anti-diabetic, anti-tumor, and regulating inflammatory properties. In this study we investigated the effect of visfatin on IFN-γ-induced apoptosis in rat pancreatic β-cells. Methods The RINm5F（rat insulinoma cell line） cells exposed to IFN-γ were treated with or without visfatin. The viability and apoptosis of the cells were assessed by using MTT and flow... 

【文章來源】：Biomedical and Environmental Sciences. 2015,28(03)

【文章頁數(shù)】：9 頁

【文章目錄】：
INTRODUCTION
METHODS
    Materials
    Cell Culture
    RNA Extraction and Quantitative Real-time PCR
    1-（4, 5-dimethylthiazol-2-yl）-3, 5-diphenylformazan Assay
    Flow-cytometric Analysis for Apoptosis
    Measurement of Caspase-3 Activity
    Western Blot Analysis
    Statistical Analysis
RESULTS
    Visfatin Protects RINm5F Cells Against IFN-γInduced Apoptosis
    VisfatinInhibitesMitochondria-dependent Apoptotic Pathway
    Visfatin Induces Activation of AMPK and ERK1/2Signaling Pathway
    Anti-apoptoticActionof Visfatin Requires Activation of AMPK and ERK1/2-mediated Signaling Pathways
DISCUSSION



本文編號：2995870