AIM: To elucidate the mechanism（s） by which S-adenosyl-L-methionine（SAM） decreases hepatitis C virus（HCV） expression.METHODS: We examined the effects of SAM on viral expression using an HCV subgenomic replicon cell culture system. Huh7 HCV-replicon cells were treated with 1 mmol/L SAM for different times（24-72 h）, then total RNA and proteins were isolated. c DNA was synthesized and real time-PCR was achieved to quantify HCV-RNA, superoxide dismutase 1 and 2（SOD-1, SOD-2） catalase, thioredoxin 1,... 

【文章來源】：World Journal of Gastroenterology. 2016年14期

【文章頁數(shù)】：12 頁


本文編號：2956349