【摘要】：最近發(fā)現(xiàn)抑制自噬會加重肝細胞內(nèi)脂肪蓄積,亞硝酸鹽可以降低高脂飼養(yǎng)小鼠體內(nèi)甘油三酯含量。因此,本實驗目的在于觀察亞硝酸鹽對單純性肝細胞脂肪變性的影響和自噬在其中的可能作用。首先用1.2 mmol·L-1油酸誘導人肝細胞系L-02細胞24 h建立肝細胞脂肪變性模型,再用0.2 mmol·L-1亞硝酸鈉加或不加三甲基腺嘌呤(3-MA)或氯喹處理24 h。肝細胞脂質(zhì)蓄積評價采用油紅O染色和細胞內(nèi)甘油三酯含量測定。1.2 mmol·L-1油酸誘導人肝細胞系L-02細胞24 h可以導致肝細胞明顯的脂肪變性。用0.2 mmol·L-1亞硝酸鈉處理L-02肝脂肪變性的細胞,可以明顯減少肝細胞脂質(zhì)蓄積,而3-MA可以減弱亞硝酸鈉的這種效應(yīng);LC3-II免疫熒光、Western blot結(jié)果顯示,亞硝酸鈉促進L-02脂肪變性細胞LC3-II熒光顆粒和蛋白表達增加,氯喹同樣可以增加這種現(xiàn)象;相差顯微鏡下顯示細胞內(nèi)空泡增多,溶酶體示蹤劑熒光顯微鏡下發(fā)現(xiàn),細胞溶酶體數(shù)量增多;電鏡顯示自噬溶酶體數(shù)量增加,氯喹可以減弱這種現(xiàn)象。這些結(jié)果表明,亞硝酸鈉能夠促進肝脂肪變性的L-02細胞自噬,促進脂質(zhì)分解,減輕肝脂肪變性細胞脂質(zhì)蓄積。
[Abstract]:Recently, it has been found that inhibition of autophagy can aggravate the accumulation of fat in hepatocytes, and nitrate can reduce the content of TG in mice fed with high fat. Therefore, the purpose of this study was to observe the effect of nitrate on simple hepatic steatosis and the possible role of autophagy in it. The hepatocytic steatosis model was established by using 1.2 mmol 路L-1 oleic acid for 24 h, and then treated with 0.2 mmol 路L-1 sodium nitroso with or without trimethyladenine (3-MA) or chloroquine for 24 h, and then treated with 0.2 mmol 路L-1 sodium nitroso or without trimethyladenine (3-MA) or chloroquine for 24 h. The lipid accumulation of hepatocytes was evaluated by oil red O staining and intracellular TG content. 1.2 mmol 路L-1 oleic acid induced obvious steatosis of human liver cell line L 鈮，

本文編號：2527202