【摘要】：最近發(fā)現(xiàn)抑制自噬會(huì)加重肝細(xì)胞內(nèi)脂肪蓄積,亞硝酸鹽可以降低高脂飼養(yǎng)小鼠體內(nèi)甘油三酯含量。因此,本實(shí)驗(yàn)?zāi)康脑谟谟^察亞硝酸鹽對(duì)單純性肝細(xì)胞脂肪變性的影響和自噬在其中的可能作用。首先用1.2 mmol·L-1油酸誘導(dǎo)人肝細(xì)胞系L-02細(xì)胞24 h建立肝細(xì)胞脂肪變性模型,再用0.2 mmol·L-1亞硝酸鈉加或不加三甲基腺嘌呤(3-MA)或氯喹處理24 h。肝細(xì)胞脂質(zhì)蓄積評(píng)價(jià)采用油紅O染色和細(xì)胞內(nèi)甘油三酯含量測(cè)定。1.2 mmol·L-1油酸誘導(dǎo)人肝細(xì)胞系L-02細(xì)胞24 h可以導(dǎo)致肝細(xì)胞明顯的脂肪變性。用0.2 mmol·L-1亞硝酸鈉處理L-02肝脂肪變性的細(xì)胞,可以明顯減少肝細(xì)胞脂質(zhì)蓄積,而3-MA可以減弱亞硝酸鈉的這種效應(yīng);LC3-II免疫熒光、Western blot結(jié)果顯示,亞硝酸鈉促進(jìn)L-02脂肪變性細(xì)胞LC3-II熒光顆粒和蛋白表達(dá)增加,氯喹同樣可以增加這種現(xiàn)象;相差顯微鏡下顯示細(xì)胞內(nèi)空泡增多,溶酶體示蹤劑熒光顯微鏡下發(fā)現(xiàn),細(xì)胞溶酶體數(shù)量增多;電鏡顯示自噬溶酶體數(shù)量增加,氯喹可以減弱這種現(xiàn)象。這些結(jié)果表明,亞硝酸鈉能夠促進(jìn)肝脂肪變性的L-02細(xì)胞自噬,促進(jìn)脂質(zhì)分解,減輕肝脂肪變性細(xì)胞脂質(zhì)蓄積。
[Abstract]:Recently, it has been found that inhibition of autophagy can aggravate the accumulation of fat in hepatocytes, and nitrate can reduce the content of TG in mice fed with high fat. Therefore, the purpose of this study was to observe the effect of nitrate on simple hepatic steatosis and the possible role of autophagy in it. The hepatocytic steatosis model was established by using 1.2 mmol 路L-1 oleic acid for 24 h, and then treated with 0.2 mmol 路L-1 sodium nitroso with or without trimethyladenine (3-MA) or chloroquine for 24 h, and then treated with 0.2 mmol 路L-1 sodium nitroso or without trimethyladenine (3-MA) or chloroquine for 24 h. The lipid accumulation of hepatocytes was evaluated by oil red O staining and intracellular TG content. 1.2 mmol 路L-1 oleic acid induced obvious steatosis of human liver cell line L 鈮，

本文編號(hào)：2527202