【摘要】：目的:探討潰瘍性結(jié)腸炎(UC)患者腸道菌群分布和細(xì)菌毒素基因的檢出情況。方法:收集UC活動(dòng)期患者32例、緩解期患者21例和45名健康對(duì)照的新鮮糞便標(biāo)本,采用直接涂片鏡檢和傳統(tǒng)細(xì)菌培養(yǎng)鑒定法分析菌群分布,利用PCR檢測糞便中6種常見的腸道致病菌毒素基因。結(jié)果:直接涂片鏡檢結(jié)果顯示,UC活動(dòng)期組菌群失調(diào)率(87.5%)高于對(duì)照組(53.3%,χ2=9.957,P=0.003),UC活動(dòng)期組菌群失調(diào)程度高于緩解期組(Z=2.501,P=0.012)。需氧培養(yǎng)結(jié)果顯示,UC組大腸埃希菌優(yōu)勢生長率(66.0%)低于對(duì)照組(88.9%,χ2=7.075,P=0.008),條件致病菌優(yōu)勢生長率(32.1%)明顯高于對(duì)照組(11.1%,χ2=6.144,P=0.013),變形桿菌屬是UC組優(yōu)勢生長率最高的條件致病菌。厭氧培養(yǎng)結(jié)果顯示,UC組益生菌和條件致病菌優(yōu)勢生長率與對(duì)照組比較差異并無統(tǒng)計(jì)學(xué)意義(P0.05)。UC組6種細(xì)菌毒素基因的總檢出率(41.5%)明顯高于對(duì)照組(22.2%,χ2=4.117,P=0.042)。結(jié)論:UC患者存在明顯的腸道菌群失調(diào)。
[Abstract]:Objective: to investigate the distribution of intestinal flora and the detection of bacterial toxin gene in patients with ulcerative colitis (UC). Methods: fresh fecal samples from 32 patients with active UC, 21 patients with remission stage and 45 healthy controls were collected. The distribution of flora was analyzed by direct smear microscopic examination and traditional bacterial culture identification method, and six common intestinal pathogenic bacteria toxin genes in feces were detected by PCR. Results: the results of direct smear examination showed that the rate of flora disorder in UC active group (87.5%) was higher than that in control group (53.3%, 蠂 2 鈮，

本文編號(hào)：2502593