【摘要】：目的:研究枸杞多糖(lycium barbarum polysaccharide,LBP)對肝細(xì)胞(LO2)脂肪變性的防治作用及其機(jī)制。方法:油酸誘導(dǎo)建立非酒精性脂肪變性肝細(xì)胞(non-alcoholic fatty liver disease,NAFLD)模型,分為正常對照組、脂肪變模型組、LBP預(yù)防組、LBP治療組。48 h后光鏡下觀察細(xì)胞內(nèi)脂質(zhì)累積情況,并檢測細(xì)胞內(nèi)谷氨酸氨基轉(zhuǎn)移酶(alanine aminostransferase,ALT)、門冬氨酸氨基轉(zhuǎn)移酶(aspartate aminostransferase,AST)、甘油三酯(triglyceride,TG)含量,及脂聯(lián)素與抵抗素的基因及蛋白表達(dá)情況。結(jié)果:光鏡下可見模型組肝細(xì)胞內(nèi)較正常對照組有大量橘紅色脂滴(P=0.000),LBP預(yù)防組和治療組與模型組比肝細(xì)胞內(nèi)的脂滴明顯減少(P=0.000、P=0.000)。LBP上調(diào)脂聯(lián)素m RNA及蛋白表達(dá)(P=0.000、P=0.014),抑制抵抗素m RNA及蛋白的表達(dá)(P=0.000、P=0.000),降低肝細(xì)胞TG、ALT、AST(P=0.000、P=0.000、P=0.000)。結(jié)論:LBP可能通過上調(diào)脂聯(lián)素m RNA及蛋白的表達(dá),下調(diào)抵抗素m RNA及蛋白表達(dá),從而有效防治NAFLD。
[Abstract]:Objective: to study the preventive and therapeutic effect of Lycium barbarum polysaccharide (lycium barbarum polysaccharide,LBP on steatosis of hepatocytes (LO2) and its mechanism. Methods: the model of nonalcoholic steatosis hepatocytes (non-alcoholic fatty liver disease,NAFLD) was induced by oleic acid and divided into normal control group, steatosis model group, LBP prevention group and LBP treatment group. 48 hours later, the intracellular lipid accumulation was observed under light microscope. The contents of intracellular glutamate aminotransferase (alanine aminostransferase,ALT), aspartate aminotransferase (aspartate aminostransferase,AST), glycerol (triglyceride,TG), and the expression of adiponectin and resistin gene and protein were detected. Results: under light microscope, there were a large number of orange lipid droplets in the hepatocytes of the model group compared with the normal control group (P 鈮，

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