【摘要】：ALD和NAFLD均存在進(jìn)展為肝硬化、肝癌、肝功能衰竭的風(fēng)險(xiǎn),已成為21世紀(jì)全球重要的公共健康問題之一。本研究第一部分收集整理了“淮安市區(qū)企業(yè)和事業(yè)單位退休職工”的體檢資料,并獲取了這一特定人群ALD和NAFLD的發(fā)病率 NAFLD確切的發(fā)病機(jī)制目前尚不清楚。miR-143是最早發(fā)現(xiàn)與NAFLD有關(guān)的miRNA,有研究表明,miR-143參與機(jī)體脂代謝的調(diào)節(jié)。因此,本研究第二部分探討了miR-143對(duì)HepG2細(xì)胞脂代謝的調(diào)節(jié)作用。 第一部分 目的：了解淮安市區(qū)企事業(yè)單位退休職工FLD的患病率及其危險(xiǎn)因素。 方法：收集2011年9月至2012年12月期間來我院體檢中心體檢的13112名退體人員的體檢資料,內(nèi)容包括：一般情況、實(shí)驗(yàn)室檢查、B型超聲檢查等。 結(jié)果：1.FLD患病人數(shù)5853人,總患病率44.63%,其中男性患病率為41.33%,女性患病率為46.62%,女性患病率顯著高于男性,差異有統(tǒng)計(jì)學(xué)意義；2.FLD組四種高脂血癥、IFG、DM、高血壓的患病率均顯著高于非FLD組；3.多因素Logistic回歸分析表明FLD的危險(xiǎn)因素包括性別(女)、ALT、IFG、高血糖、TG、LDL-C、高血壓。 結(jié)論：淮安市區(qū)企事業(yè)單位退休人員FLD的患病率較高,且多與其它代謝類疾病并存,必須加強(qiáng)綜合防治。 目的：探討miR-143對(duì)HepG2細(xì)胞脂代謝的調(diào)節(jié)作用。 方法：體外培養(yǎng)HepG2細(xì)胞,培養(yǎng)液中加入FFA,同時(shí)轉(zhuǎn)染miR-143及其對(duì)照,采用qRT-PCR技術(shù)檢測HepG2細(xì)胞轉(zhuǎn)染miR-143的情況。采用油紅O染色觀察HepG2細(xì)胞內(nèi)脂質(zhì)積聚的情況。采用脂蛋白脂肪酶-甘油磷酸氧化酶-過氧化物酶-4-氨基安替比林和酚法(GPO-PAP)法在奧林巴斯(OLYMPUS) AU640全自動(dòng)生化儀上檢測HepG2細(xì)胞內(nèi)TG含量。 結(jié)果：0.25mmol/L FFA可導(dǎo)致HepG2細(xì)胞內(nèi)TG明顯積聚,且呈時(shí)間依賴性,miR-143過表達(dá)可以顯著抑制高FFA狀態(tài)下HepG2內(nèi)脂質(zhì)的積聚。 結(jié)論：miR-143可能參與了肥胖狀態(tài)下肝臟細(xì)胞脂類代謝紊亂的調(diào)節(jié)。
[Abstract]:Both ALD and NAFLD have the risk of liver cirrhosis, liver cancer and liver failure, which has become one of the most important public health problems in the 21st century. The first part of this study collected and sorted out the physical examination data of "retired employees of enterprises and institutions in Huai'an City". The exact pathogenesis of the incidence of ALD and NAFLD in this particular population has not been known yet. It has been suggested that miR-143 is involved in the regulation of lipid metabolism in the body, which is the first miRNA, to be found to be associated with NAFLD. Therefore, in the second part of this study, we investigated the effect of miR-143 on lipid metabolism in HepG2 cells. Objective: to investigate the prevalence of FLD and its risk factors among retired employees in Huai'an city. Methods: from September 2011 to December 2012, the physical examination data of 13112 retired persons who came to our hospital from September 2011 to December 2012 were collected, including general condition, laboratory examination, B-mode ultrasound examination and so on. Results: the total prevalence rate of 1.FLD was 44.63%, of which 41.33% was male and 46.62% was female. The prevalence rate in female was significantly higher than that in male (P < 0.05). The prevalence of four kinds of hyperlipidemia and IFG,DM, hypertension in 2.FLD group was significantly higher than that in non-FLD group; Multivariate Logistic regression analysis showed that the risk factors of FLD included sex (female), ALT,IFG, hyperglycemia and TG,LDL-C, hypertension. Conclusion: the prevalence of FLD in retirees of enterprises and institutions in Huaian city is higher and coexists with other metabolic diseases, so it is necessary to strengthen comprehensive prevention and treatment. Aim: to investigate the effect of miR-143 on lipid metabolism in HepG2 cells. Methods: HepG2 cells were cultured in vitro. FFA, was added into the culture medium to transfect miR-143 and its control. QRT-PCR technique was used to detect the transfection of HepG2 cells into miR-143. Lipid accumulation in HepG2 cells was observed by oil red O staining. Lipoprotein lipase-glycerophosphate oxidase-peroxidase-4-amino-antipyrine and phenol (GPO-PAP) method were used to detect the TG content in HepG2 cells on Olympus (OLYMPUS) AU640 automatic biochemical instrument. Results: 0.25mmol/L FFA could induce the accumulation of TG in HepG2 cells in a time-dependent manner. The overexpression of miR-143 could significantly inhibit the accumulation of lipid in HepG2 at high FFA level. Conclusion: miR-143 may be involved in the regulation of lipid metabolism disorder in obese liver cells.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R575.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王輝,王江濱;肝炎病毒感染與酒精性肝硬化關(guān)系的研究(附182例酒精性肝病臨床病例報(bào)告)[J];白求恩醫(yī)科大學(xué)學(xué)報(bào);1998年06期

2 劉陽,遲寶榮;酒精性肝硬化237例臨床分析[J];吉林醫(yī)學(xué);2004年04期

3 萇新明;，

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