【摘要】：目的：探討荔枝核總黃酮(total flavone from Litchi Chinensis Sonn,TFL)對大鼠肝纖維化PDGF及TNF-α因子的影響。方法：SD雄性大鼠60只，，隨機分為空白組、模型組、水飛薊賓（SIL）組(50mg kg-1d-1)、TFL高劑量組(400mg kg-1d-1)、TFL中劑量組(200mg kg-1d-1)、TFL低劑量組(100mg kg-1d-1)六組。采用二甲基亞硝胺DMN腹腔注射制備SD大鼠進行肝纖維化造模，造模4周，造模同時SIL、TFL等四組灌胃給藥干預(yù)，6周后處死大鼠，觀察大鼠肝纖維化水平，用放射免疫法測定模型組、SIL組和TEL組大鼠血清中層粘蛋白（LN）、透明質(zhì)酸（HA）及Ⅲ型前膠原（PCⅢ）水平，用HE染色法和Masson染色觀察大鼠肝組織鏡下病理變化纖維化水平，采用PCR法檢測SIL組和TFL組PDGF、TNF因子表達水平變化，用SPSS11.0軟件對數(shù)據(jù)進行統(tǒng)計分析。結(jié)果：與模型組大鼠比較, SIL和TFL大、中劑量治療后大鼠血清中HA、LN和PCⅢ的水平顯著降低（P＜0.01）；肝組織內(nèi)纖維化程度降低,肝小葉結(jié)構(gòu)趨于正常；TFL大、中劑量給藥組和水飛薊素組血清PDGF及TNF-α水平均顯著低于模型組（P＜0.05）；兩組大鼠肝組織PDGF及TNF-α表達明顯降低(P＜0.05)。TFL中劑量給藥組與對照組比較血清HA、LN和PCⅢ的水平、肝纖維化程度、PDGF及TNF-α表達水平均無明顯差異(P＞0.05)。結(jié)論：TFL可以下調(diào)二甲基亞硝胺肝纖維化大鼠的PDGF、TNF-α細胞因子表達水平，具有明顯的抗大鼠肝纖維化作用。
[Abstract]:Objective: to investigate the effects of total flavonoids (total flavone from Litchi Chinensis Sonn,TFL on PDGF and TNF- 偽 in rat liver fibrosis. Methods: sixty male SD rats were randomly divided into blank group, model group and silybin (SIL) group (50mg kg-1d-1), TFL high dose group (400mg kg-1d-1), TFL medium dose group) 200mg kg-1d-1), TFL low dose group (100mg kg-1d-1) group. SD rats were induced by intraperitoneal injection of dimethylnitrosamine (DMN) to model liver fibrosis for 4 weeks. Four groups of rats, such as SIL,TFL, were given intragastric administration of dimethylnitrosamine. After 6 weeks, the rats were killed to observe the level of hepatic fibrosis. The levels of serum (LN), hyaluronic acid (HA) and type 鈪

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