【摘要】：目的探討枯草桿菌二聯(lián)活菌腸溶膠囊(美常安)對潰瘍性結腸炎(UC)大鼠結腸促炎癥性細胞因子白細胞介素(IL)-1β和細胞黏附分子CD44表達的影響及其作用機制。方法采用2,4,6-三硝基苯磺酸(TNBS)/乙醇法灌腸建立UC大鼠模型。將SD大鼠隨機分成正常對照組、模型組、美常安治療組。觀察各組疾病活動指數(shù)(DAI)、結腸組織病理學評分(HPS)。造模及美常安干預7 d后處死大鼠,取結腸行HE染色,觀察鏡下結腸病理變化。RT-PCR法檢測各組大鼠腸黏膜細胞因子IL-1βmRNA表達,流式細胞儀檢測各組大鼠結腸組織中黏附分子CD44的含量。結果模型組較正常對照組大鼠結腸黏膜可見膿性分泌物、糜爛及潰瘍,美常安干預后顯著改善UC大鼠結腸黏膜的病理變化,DAI和HPS評分下降顯著,并明顯降低UC大鼠IL-1βmRNA和CD44的表達(P0.05)。結論枯草桿菌二聯(lián)活菌可能通過下調UC大鼠IL-1βmRNA和CD44的表達而發(fā)揮干預作用。
[Abstract]:Objective to investigate the effect of Bacillus subtilis enteric-coated capsule (Meichang an) on the expression of interleukin-1 尾 (IL) 尾) and cell adhesion molecule (CD44) in colonic inflammatory cytokines (IL) 尾) and adhesion molecules (CD44) in rats with ulcerative colitis (UC). SD rats were randomly divided into normal control group, model group and mechangan treatment group. The disease activity index (DAI),) and colonic histopathology score (HPS).) were observed in each group. After 7 days of intervention, the rats were killed, the colons were stained with HE, and the expression of intestinal mucosal cytokine IL-1 尾 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). The content of adhesion molecule CD44 in colonic tissue of rats in each group was detected by flow cytometry. Results the mucosal purulent secretion, erosion and ulceration were observed in the model group compared with the normal control group. The pathological changes of colonic mucosa in UC rats were significantly improved after the intervention of Mechangan. The scores of Dai and HPS decreased significantly in the model group. The expression of IL-1 尾 mRNA and CD44 in UC rats was significantly decreased (P 0.05). Conclusion Bacillus subtilis combined with Bacillus subtilis may play an intervention role by down-regulating the expression of IL-1 尾 mRNA and CD44 in UC rats.
【作者單位】： 桂林市第二人民醫(yī)院消化內科;
【基金】：廣西壯族自治區(qū)衛(wèi)生廳自籌經費科研課題(No.Z2014269)
【分類號】：R574.62

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