本文選題：急性腹瀉 + 副溶血弧菌��； 參考：《浙江大學》2014年碩士論文

【摘要】：目的： 副溶血弧菌(Vibrio parahaemolyticus, VP)是一種革蘭陰性嗜鹽性弧菌,廣泛存在于海水和海產品中。1950年在日本副溶血弧菌首次發(fā)現并被認為是引起食物中毒的重要致病菌,在我國,副溶血弧菌感染引發(fā)的暴發(fā)事件居微生物食源性疾病爆發(fā)的首位。浙江寧波位于我國東南沿海,海產品消費比較盛行,人們又喜歡食用生的或者未煮熟的海產品,因此副溶血弧菌是引起食物中毒最主要的病原菌。本課題通過收集寧波北侖區(qū)急性腹瀉患者糞便標本,通過檢測血清學凝集試驗,可以知道寧波北侖區(qū)溶血弧菌血清型構成譜；通過毒力基因檢測及分子分型分析,可以為副溶血弧菌感染的防控提供分子流行病學依據,這為開展副溶血弧菌的持續(xù)監(jiān)測研究提供依據,并為進一步的研究工作奠定基礎。 方法： 2012年4月至2012年10月,從北侖區(qū)人民醫(yī)院采集急性腹瀉患者的糞便標本,用常規(guī)微生物檢驗程序對常見腸道致病菌進行分離培養(yǎng)及鑒定。對分離的副溶血弧菌菌株開展血清學凝集試驗、利用PCR技術檢測毒力基因檢測及多位點序列分型(MLST)分析。 結果： 1.2012年4-10月共收集腹瀉患者的糞便共962份,共分離出副溶血弧菌71株,分離率為7.4%(71/962),列細菌性病原第二位。感染病例男女性別比為1.73:1(45例：26例),年齡范圍為16-61歲,其中主要集中在20-45歲(69.0%,49/71)。 2.本次研究共有16種O、K血清型組合,其中03:K6型是最主要的血清型,共44株,占62.0%；04:K8(5株,7.0%)、O1:K56(3株,4.2%),另有3(4.2%)株未鑒定出O、K型別,歸類于OUT:KUT型。 3.利用PCR對71株副溶血弧菌進行tdh.trh.tlh毒力基因檢測發(fā)現：所有副溶血弧菌tlh基因均陽性,98.6%的菌株(70株)tdh基因陽性,僅3株為trh陽性。基因型表現為：tlh+tdh+trh-菌株67株,tlh+tdh+trh+菌株3株和tlh+tdh-trh-菌株1株。通過對大流行群菌株遺傳標志性基因的檢測發(fā)現,寧波北侖區(qū)急性腹瀉患者感染的副溶血弧菌主要是大流行菌株(tdh+,trh-和toxRS/new+),共47株(66.2%)；非大流行菌株共24株(33.8%)。36株大流行菌株攜帶orf8基因,占所有大流行菌株的76.6%。 4.在本次研究中,71株急性腹瀉患者分離的副溶血弧菌進行副溶血弧菌MLST分析,全部成功分型,共分為13個STs,其中ST3共45株(63.4%),ST885株,ST479、ST8和ST120各4株,另外還包括ST176、ST199、ST216、ST332、ST417、 ST610、ST712和ST80等8種STs。 結論： 1.本地區(qū)急性腹瀉患者中副溶血弧菌檢出率高達7.4%,因此在本地區(qū)腹瀉病例中有必要開展副溶血弧菌的檢測,降低副溶血性弧菌引起的食源性疾病的發(fā)生和傳播的有效途徑。 2.TDH是寧波北侖地區(qū)副溶血弧菌最主要的致病因子,大流行株是本地區(qū)最主要的流行株,血清型03:K6的副溶血弧菌是其優(yōu)勢菌株。 3.通過MLST分型發(fā)現寧波北侖區(qū)急性腹瀉患者中副溶血弧菌ST3最多,是優(yōu)勢型別。主要優(yōu)勢型別與世界上許多國家流行的型別一致,但同時又存在多種亞型。副溶血弧菌流行亞型的多樣性是其在寧波北侖區(qū)呈高發(fā)流行的一個主要原因。
[Abstract]:Objective:
Vibrio parahaemolyticus (Vibrio parahaemolyticus, VP), a gram-negative halophilic Vibrio, is widely found in sea water and marine products for the first time in Japanese Vibrio parahaemolyticus (Vibrio parahaemolyticus) and is considered to be an important pathogen for food poisoning. In China, the outbreak of Vibrio parahaemolyticus caused by the outbreak of Vibrio parahaemolyticus is in the outbreak of food borne diseases. Zhejiang Ningbo is located in the southeast coast of China, and the consumption of seafood is more prevalent. People like to eat raw or uncooked seafood. Therefore, Vibrio parahaemolyticus is the main cause of food poisoning. By collecting the stool specimens from patients with acute diarrhea in Beilun District of Ningbo, the serological agglutination test can be carried out. To know the composition spectrum of serotype of Vibrio haemolyticus in Beilun District of Ningbo, and to provide molecular epidemiological basis for the prevention and control of Vibrio parahaemolyticus through the detection of virulence gene and molecular typing, which provides the basis for the continuous monitoring and research of Vibrio parahaemolyticus and for further research work.
Method:
From April 2012 to October 2012, the fecal specimens of patients with acute diarrhea were collected from the people's Hospital of Beilun District, and the common intestinal pathogenic bacteria were isolated, cultured and identified by routine microbiological test procedure. The serological agglutination test was carried out on the isolated Vibrio parahaemolyticus strain, and the PCR technique was used to detect the virulence gene and the multipoint sequence classification (M LST) analysis.
Result:
A total of 962 diarrhoea patients were collected in 1.2012 years and 4-10 months, and 71 strains of Vibrio parahaemolyticus were isolated. The separation rate was 7.4% (71/962) and second were bacterial pathogens. The sex ratio of male and female in the infected cases was 1.73:1 (45 cases, 26 cases) and the age range was 16-61 years old, mainly in 20-45 years (69%, 49/71).
2. a total of 16 kinds of O, K serotype combination, of which type 03:K6 is the most important serotype, a total of 44, 62%, 04:K8 (5, 7%), O1:K56 (3, 4.2%), and 3 (4.2%) strains did not identify O, K type, classified in OUT:KUT type.
3. tdh.trh.tlh virulence gene detection of Vibrio parahaemolyticus by PCR showed that all Vibrio parahaemolyticus TLH gene was positive, 98.6% strains (70 strains) were TDH positive and only 3 were TRH positive. The genotypes were 67 strains of tlh+tdh+trh- strain, 3 strains of tlh+tdh+trh+ strain and 1 strains of tlh+tdh-trh- strain. The detection of genetic markers found that the main strains of Vibrio parahaemolyticus infected by acute diarrhea in Beilun District, Ningbo, were mainly pandemic strains (tdh+, trh- and toxRS/new+), 47 (66.2%), 24 strains of non - pandemic strains (33.8%).36 strains carried the ORF8 gene, accounting for the 76.6%. of the pandemic strain.
4. in this study, 71 strains of Vibrio parahaemolyticus isolated from Vibrio parahaemolyticus isolated from acute diarrhea patients were analyzed with Vibrio parahaemolyticus (Vibrio parahaemolyticus MLST), all of which were divided into 13 STs, including 45 ST3 (63.4%), ST885, ST479, ST8 and ST120, including ST176, ST199, ST216, ST332, ST417, ST417, 8, etc.
Conclusion:
1. the detection rate of Vibrio parahaemolyticus in patients with acute diarrhea in the region is up to 7.4%. Therefore, it is necessary to detect Vibrio parahaemolyticus in local diarrhea cases and reduce the effective way of the occurrence and transmission of foodborne diseases caused by Vibrio parahaemolyticus.
2.TDH is the most important pathogenic factor of Vibrio parahaemolyticus in Beilun, Ningbo. The pandemic strain is the main epidemic strain in the region, and Vibrio parahaemolyticus of serotype 03:K6 is the dominant strain.
3. it was found that Vibrio parahaemolyticus ST3 was the most dominant type of parahaemolyticus in acute diarrhea patients in Beilun District of Ningbo Province, which was the dominant type. The main dominant type was in accordance with the popular types in many countries in the world, but there were many subtypes at the same time. The diversity of Vibrio parahaemolyticus epidemic subtype is one of the main reasons for its high prevalence in Beilun District of Ningbo.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R446.5;R572

【共引文獻】

相關期刊論文 前1條

1 朱鍵;張永標;楊曉燕;梁彩倩;符永玫;羅敏琪;;奇異變形桿菌感染臨床分布與耐藥性特征分析[J];實用醫(yī)學雜志;2015年05期

相關碩士學位論文 前1條

1 楊燕;浙江省沙門菌的分子分型和流行病學研究[D];浙江大學;2014年

，

本文編號：2090023