本文選題：潰瘍性結(jié)腸炎 + 糞菌移植��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年12期

【摘要】：目的研究糞菌移植(fecal microbiota transplantation,FMT)對(duì)硫酸葡聚糖(dextran sulfate sodium,DSS)誘導(dǎo)的潰瘍性結(jié)腸炎小鼠的治療作用。方法將92只7周齡雌性C57BL/6小鼠,按照隨機(jī)數(shù)字表法分為4組,每組23只:對(duì)照組正常飲水,模型組飲用2%DSS 7 d,在第8天時(shí)換成不含DSS的滅菌純凈水飲用7 d,FMT預(yù)防組在飲用DSS建模的同時(shí)給予FMT灌胃處理,FMT治療組在飲用7 d DSS模型建成后進(jìn)行FMT灌胃處理7 d。記錄各組小鼠體質(zhì)量變化和疾病活動(dòng)度,并收集每日糞便進(jìn)行腸道菌群分析。在第7、14天末分別處死10只小鼠,采集血液標(biāo)本進(jìn)行TNF-α和D-乳酸測(cè)量,留取結(jié)腸組織分別進(jìn)行HE染色、透射電鏡觀察,免疫組化染色檢測(cè)緊密連接相關(guān)蛋白ZO-1和Occludin的表達(dá)。結(jié)果 FMT治療組小鼠體質(zhì)量、疾病活動(dòng)程度和結(jié)腸長(zhǎng)度較DSS誘導(dǎo)結(jié)腸炎小鼠模型組顯著改善(P0.01)。FMT治療組血清D-乳酸和TNF-α水平較模型組明顯降低(P0.05,0.01)。透射電鏡可見FMT治療組結(jié)腸上皮的損害及細(xì)胞間緊密連接結(jié)構(gòu)和上皮完整性較模型組顯著改善。FMT治療及預(yù)防處理后ZO-1和Occludin的表達(dá)較模型組明顯增高(P0.05,P0.01)。FMT預(yù)防及治療都能顯著改善DSS引起的腸道菌群改變,促進(jìn)乳桿菌屬增殖而抑制擬桿菌屬和顫螺菌屬的生長(zhǎng)。結(jié)論FMT預(yù)防及治療都能顯著改善硫酸葡聚糖引起的腸道菌群改變,促進(jìn)乳桿菌屬增殖而抑制擬桿菌屬和顫螺菌屬的生長(zhǎng)。
[Abstract]:Objective to study the therapeutic effect of fecal bacteria transplantation of (fecal microbiota transplants on mice with ulcerative colitis induced by dextran sulfate (dextran sulfate sodium (DSS). Methods Ninety-two 7-week-old female C57BL / 6 mice were randomly divided into 4 groups, 23 in each group. The model group drank 2 DSS for 7 days. On the 8th day, the model group was replaced with sterilized water without DSS for 7 days. The prevention group was treated with FMT for 7 days after drinking DSS. Changes in body mass and disease activity were recorded and daily feces were collected for intestinal flora analysis. At the end of the 7th day, 10 mice were killed. TNF- 偽 and D- lactic acid were measured in blood samples, HE staining, transmission electron microscopy and immunohistochemical staining were used to detect the expression of tight junction protein ZO-1 and Occludin. Results the body weight, disease activity and colonic length in FMT group were significantly improved than those in DSS induced colitis model group (P0.01). The serum levels of D- lactic acid and TNF- 偽 in FMT group were significantly lower than those in model group (P0.05 + 0.01). Transmission electron microscope showed that the lesion of colonic epithelium, the structure of intercellular tight junction and the integrity of epithelium in FMT group were significantly improved compared with model group. The expression of ZO-1 and Occludin in FMT treatment group was significantly higher than that in model group (P0.05, P0.01). FMT prevention and treatment. Can significantly improve the intestinal flora changes caused by DSS, Promote the proliferation of Lactobacillus and inhibit the growth of Bacteroides and Helicobacterium. Conclusion both prevention and treatment of FMT can significantly improve the intestinal flora changes induced by dextran sulfate, promote the proliferation of Lactobacillus and inhibit the growth of Bacteroides and Helicobacterium.
【作者單位】： 第三軍醫(yī)大學(xué)大坪醫(yī)院野戰(zhàn)外科研究所消化內(nèi)科;
【基金】：第三軍醫(yī)大學(xué)大坪醫(yī)院野戰(zhàn)外科研究所臨床科研基金(2014YLC05) 重慶市科委社會(huì)民生基金(CSTC2015shmszx120047)~~
【分類號(hào)】：R574.62

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