發(fā)布時(shí)間：2018-06-22 01:12

  本文選題：肝硬化 + 門靜脈高壓��； 參考：《蚌埠醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:觀察茶多酚(TP)對(duì)CC14所致大鼠肝硬化的保護(hù)作用,并探討其可能的作用機(jī)制。方法:雄性SD大鼠40只,隨機(jī)分為4組,正常對(duì)照組(N組)、正常加茶多酚組(N+TP組)、肝硬化組(PHT組)、茶多酚干預(yù)組(PHT+TP組)。PHT組及PHT+TP組分別按0.3ml/100g皮下注射CC14,每周2次,其余兩組皮下注射等量生理鹽水。在造模12周后,N+TP組和PHT+TP組均用茶多酚[150mg/(kg.d)]灌胃治療。16周末檢測(cè)各項(xiàng)指標(biāo)(血清ALT、AST,氧化應(yīng)激指標(biāo),平均動(dòng)脈壓,門靜脈壓力),處死各組大鼠,留取肝臟組織,觀察各組大鼠肝組織病理改變(肝組織HE染色),測(cè)定α-SMA,并比較各組間的區(qū)別有無統(tǒng)計(jì)學(xué)意義。結(jié)果:茶多酚干預(yù)組與肝硬化組相比,茶多酚治療組的肝纖維化大鼠肝臟中α-SMA水平及ALT和AST表達(dá)均降低,組織病理觀察顯示茶多酚干預(yù)組肝硬化程度比肝硬化組輕,門脈壓力下降。結(jié)論:茶多酚能減輕CC14誘導(dǎo)大鼠發(fā)生的肝損傷與肝硬化,其機(jī)制可能為通過降低肝組織氧化應(yīng)激水平而發(fā)揮護(hù)肝作用。
[Abstract]:Aim: to observe the protective effect of tea polyphenols (TP) on CC14 induced liver cirrhosis in rats and to explore its possible mechanism. Methods: forty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group (N group), normal tea polyphenol group (N TP group), cirrhosis group (PHT group), tea polyphenol intervention group (PHT TP group). The other two groups were subcutaneously injected with the same amount of normal saline. After 12 weeks of model making, the rats in the N TP group and the 150mg/ TP group were treated with 150mg/ (kg.d). At the end of week 16, the serum alt AST, oxidative stress index, mean arterial pressure and portal vein pressure were measured. The rats in each group were killed and the liver tissue was taken. The pathological changes of liver tissue (HE staining) and 偽 -SMAwere observed in each group, and the difference between each group was statistically significant. Results: the level of 偽 -SMA and the expression of alt and AST in the liver of the tea polyphenol treated group were lower than that of the cirrhosis group. Histopathological observation showed that the liver cirrhosis degree in the tea polyphenol treated group was less than that in the cirrhosis group. Portal pressure decreased. Conclusion: tea polyphenols can attenuate the liver injury and cirrhosis induced by CC14 in rats. The mechanism of tea polyphenols may play a protective role by reducing the level of oxidative stress in liver tissue.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R575.2

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本文編號(hào)：2050872