本文選題：芒果苷 + 果糖��； 參考：《第三軍醫(yī)大學學報》2015年20期

【摘要】：目的研究芒果苷改善高果糖誘導的Hep G2細胞內(nèi)甘油三酯沉積的可能機制。方法低糖(1 g/L葡萄糖)條件下培養(yǎng)的Hep G2細胞分為對照組(1 g/L葡萄糖,無果糖)、果糖組(1 g/L葡萄糖+30 mmol/L果糖)、果糖+芒果苷組(同時加入葡萄糖濃度1 g/L+30 mmol/L果糖+不同劑量的芒果苷,使其藥物終濃度分別為6.25、12.5、25、50μmol/L),藥物干預24 h之后,油紅O染色觀察細胞內(nèi)脂滴的沉積情況,酶法測定細胞內(nèi)甘油三酯(TG)的含量,Real-time PCR檢測碳水化合物反應元件結(jié)合蛋白(ChREBP)、固醇調(diào)節(jié)元件結(jié)合蛋白1c(SREBP-1c)、肝型丙酮酸激酶(LPK)、二酯酰甘油�；D(zhuǎn)移酶2(DGAT-2)mRNA表達的變化。結(jié)果油紅O染色結(jié)果顯示,與對照組相比,果糖組的Hep G2細胞脂滴顯著增多,細胞內(nèi)TG含量也明顯升高(P0.05)。與果糖組對比,低劑量的芒果苷對果糖導致的細胞內(nèi)脂滴的沉積無影響,較高劑量的芒果苷(25μmol/L和50μmol/L)使細胞內(nèi)的脂滴數(shù)量明顯減少,細胞內(nèi)TG含量顯著降低(P0.05),以50μmol/L的芒果苷干預效果最好。Real-time PCR結(jié)果顯示,與對照組相比,果糖組Hep G2細胞ChREBP、SREBP-1c、LPK、DGAT-2 mRNA明顯升高,50μmol/L芒果苷能夠下調(diào)Hep G2細胞ChREBP、LPK、DGAT-2 mRNA的高表達(P0.05),但對SREBP-1c mRNA表達影響不明顯。結(jié)論芒果苷能夠改善高果糖誘導的Hep G2細胞內(nèi)TG的沉積,可能與抑制脂質(zhì)合成相關(guān)基因ChREBP、LPK、DGAT-2 mRNA的表達密切相關(guān)。
[Abstract]:Objective to study the possible mechanism of mangiferin in improving triglyceride deposition in Hep G2 cells induced by high fructose. Methods HepG2 cells cultured with low glucose (1 g / L glucose) were divided into control group (1 g / L glucose). Without fructose, the fructose group with 1 g / L glucose 30 mmol / L fructose, fructose mangiferin group (1 g / L 30 mmol / L fructose with different doses of mangiferin was added at the same time, the final drug concentration was 6.25% 12.55% 25 渭 mol / L, respectively. After 24 hours of drug intervention, Oil red O staining was used to observe the deposition of lipid droplets in cells. Real-time PCR was used to detect the expression of carbohydrate response element binding protein (CHREBPN), steroid regulatory element binding protein (1cctbbp1), hepatic pyruvate kinase (LPKK) and diester acylglyceryl transferase (2DGAT-2). Results the results of oil red O staining showed that the lipid droplets of Hep G2 cells in fructose group were significantly higher than those of the control group, and the content of TG in the cells was also significantly higher than that in the control group. Compared with fructose group, low dose mangiferin had no effect on lipid droplet deposition in cells induced by fructose, and high dose mangiferin 25 渭 mol / L and 50 渭 mol / L) significantly reduced the number of lipid droplets in cells. The content of TG in cells decreased significantly (P 0.05). Compared with the control group, 50 渭 mol / L mangiferin had the best effect on real-time PCR. Fructose treated Hep G2 cell line ChREBPnSREBP-1 / LPKP-1 / LPKDGAT-2mRNA increased by 50 渭 mol / L mangiferin could down-regulate the high expression of P0.05mRNA in Hep G2 cells, but had no significant effect on the expression of SREBP-1c mRNA. Conclusion mangiferin can improve the deposition of TG in Hep G2 cells induced by high fructose, which may be related to the inhibition of the expression of LPKN DGAT-2 mRNA in Hep G2 cells induced by high fructose.
【作者單位】： 重慶醫(yī)科大學基礎醫(yī)學院干細胞與組織工程研究室;重慶醫(yī)科大學生命科學院脂糖代謝重點實驗室;重慶醫(yī)科大學中醫(yī)藥學院中醫(yī)藥研究室;
【基金】：國家自然科學基金面上項目(81374033)~~
【分類號】：R575.5

【共引文獻】

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1 俞若熙;基于陰虛、陽虛體質(zhì)基因表達的健康狀態(tài)微觀辨識研究[D];北京中醫(yī)藥大學;2013年

2 周露婷;肝臟ZBTB20調(diào)節(jié)脂質(zhì)代謝和胰島素敏感性的機制研究[D];第二軍醫(yī)大學;2013年

3 張玉皓;LPK基因在非酒精性脂肪肝中的表觀遺傳學研究[D];復旦大學;2012年

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本文編號：2002701