本文選題：胃上皮細(xì)胞 + 替普瑞酮��； 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的：觀察替普瑞酮對(duì)人正常胃上皮細(xì)胞增殖、遷移及凋亡等生物學(xué)現(xiàn)象的作用 方法： 1、體外培養(yǎng)人正常胃上皮細(xì)胞GES。 2.、用MTT法檢測(cè)不同濃度替普瑞酮對(duì)胃上皮細(xì)胞增殖的作用及確定替普瑞酮最佳作用濃度。 3、用替普瑞酮最佳作用濃度培養(yǎng)胃正常上皮細(xì)胞,以無水乙醇溶劑組作為對(duì)照,采用TRANSWELL實(shí)驗(yàn)、劃痕實(shí)驗(yàn)檢測(cè)替普瑞酮對(duì)胃上皮細(xì)胞運(yùn)動(dòng)能力及遷移的作用。 4、以無水乙醇溶劑組作為對(duì)照,采用細(xì)胞流式分析方法檢測(cè)替普瑞酮對(duì)胃上皮細(xì)胞凋亡的作用。 結(jié)果： 1、替普瑞酮作用于胃上皮細(xì)胞24小時(shí)后,開始出現(xiàn)促進(jìn)胃上皮細(xì)胞增殖的作用,從10uM-80uM隨著濃度增加,促進(jìn)作用越明顯,而80uM-320uM隨著濃度增加,促進(jìn)作用無明顯改變,同時(shí),確定藥物最佳作用濃度為80uM。 2、替普瑞酮處理胃上皮細(xì)胞24小時(shí)后,TRANSWELL小室膜下藍(lán)染的細(xì)胞明顯多于對(duì)照組,溶劑對(duì)照組的遷移率為1.328,加藥組的遷移率為3.338,加藥組遷移率明顯高于對(duì)照組(P0.01),因此,加藥后胃上皮細(xì)胞的遷移率明顯增加。 3、替普瑞酮處理胃上皮細(xì)胞8小時(shí)后,加藥組劃痕區(qū)域細(xì)胞遷移率為0.54,對(duì)照組為0.38,加藥組與對(duì)照組遷移率無明顯差異(P0.05),而替普瑞酮作用24h后,加藥組遷移率為1.00,對(duì)照組遷移率為0.72,加藥組遷移率明顯高于對(duì)照組(P0.05),因此,加藥后胃上皮細(xì)胞的遷移率增加。 4、替普瑞酮處理胃上皮細(xì)胞48小時(shí)后,加藥組與對(duì)照組相比,早期凋亡細(xì)胞及晚期凋亡細(xì)胞均明顯減少,加藥組細(xì)胞凋亡率為11.9%,對(duì)照組細(xì)胞凋亡率為24.54%,加藥組細(xì)胞的凋亡率低于對(duì)照組(P0.05)。 結(jié)論： 1、替普瑞酮能夠促進(jìn)人正常胃上皮細(xì)胞增殖。 2、替普瑞酮能夠促進(jìn)人正常胃上皮細(xì)胞遷移。 3、替普瑞酮能夠抑制人正常胃上皮細(xì)胞凋亡。
[Abstract]:Objective: to observe the effects of tipranone on proliferation, migration and apoptosis of human normal gastric epithelial cells. Methods: 1. Human normal gastric epithelial cells were cultured in vitro. 2. The effects of different concentrations of tipranone on the proliferation of gastric epithelial cells were detected by MTT assay and the optimum concentration of tipranone was determined. 3. Normal gastric epithelial cells were cultured with the optimal concentration of tipranone. The effects of tipranone on the motility and migration of gastric epithelial cells were detected by TRANSWELL test and scratch test. 4. The effect of tipranone on apoptosis of gastric epithelial cells was detected by flow cytometry with anhydrous ethanol solvent as control group. Results: 1. After treated with tipranone for 24 hours, the proliferation of gastric epithelial cells began to be promoted. With the increase of 10uM-80uM concentration, the promoting effect was more obvious, but 80uM-320uM had no obvious change with the increase of concentration, at the same time, the effect of tipranone on the proliferation of gastric epithelial cells began to appear. The optimum concentration of the drug was determined to be 80 UM. 2. After treated with tipranone for 24 hours, the number of blue stained cells in the subventricular membrane of TRANSWELL was significantly higher than that in the control group, the mobility of the solvent control group was 1.328, the migration rate of the drug added group was 3.338, and the migration rate of the drug added group was significantly higher than that of the control group (P 0.01). The migration rate of gastric epithelial cells was significantly increased after administration. 3. After treated with tipranone for 8 hours, the cell migration rate of scratched area was 0.54 in the treatment group and 0.38 in the control group. There was no significant difference in the migration rate between the two groups (P 0.05). However, after 24 hours of treatment with tipranone, the migration rate of the cells in the scratch area was 0.54, and that in the control group was 0.38. The mobility of gastric epithelial cells was significantly higher in the admixture group than that in the control group (1.00 vs 0.72). (4) after treated with tipranone for 48 hours, the number of early apoptotic cells and late apoptotic cells in the treatment group was significantly lower than that in the control group. The apoptotic rate of the drug added group was 11.9% and that of the control group was 24.54%. The apoptosis rate of the drug added group was lower than that of the control group (P 0.05). Conclusion: 1. Tipranone can promote the proliferation of normal gastric epithelial cells. 2, tipranone can promote the migration of normal gastric epithelial cells. 3, tipranone can inhibit the apoptosis of normal gastric epithelial cells.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R57

【共引文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 裴連薇;替普瑞酮治療慢性非萎縮性胃炎伴糜爛的臨床研究[D];大連醫(yī)科大學(xué);2012年

2 陳宇;林一帆教授中西醫(yī)結(jié)合診治胃癌癌前病變經(jīng)驗(yàn)總結(jié)[D];遼寧中醫(yī)藥大學(xué);2013年

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本文編號(hào)：1911616