發(fā)布時(shí)間：2018-05-17 06:11

  本文選題：高溫高濕高血糖顱腦火器傷 + 實(shí)驗(yàn)性急性末端回腸炎　； 參考：《南華大學(xué)》2014年碩士論文

【摘要】：目的：探討TNF-α、IL-6的表達(dá)水平在實(shí)驗(yàn)性急性末端回腸炎中的變化。 方法：選取30只健康成年的雄性雜種犬，運(yùn)用隨機(jī)數(shù)字抽樣法分成6組：正常對(duì)照組（A組）、高溫高濕高血糖組（B組）、正常血糖顱腦火器傷組（C組）、高血糖顱腦火器傷組（D組）、正常血糖濕熱下顱腦火器傷組（E組）、高血糖濕熱下顱腦火器傷組（F組），每組5只。將后四組（C、D、E、F組）給予手槍彈顱腦火器傷造模。在實(shí)驗(yàn)組（B、C、D、E、F組）共五組造模后，將犬立刻仰臥位固定在手術(shù)臺(tái)上，并行氣管插管、心電監(jiān)護(hù)、心肺復(fù)蘇，觀測(cè)各組犬生命體征的變化。觀察過(guò)程中，除心臟擠壓及使用球囊呼吸器搶救外，不做其他處理。在無(wú)菌條件下，立即開(kāi)腹，收集距回盲部1-3cm處的末端回腸組織。肉眼大體觀察，石蠟包埋后行蘇木精-伊紅染色法（HE染色）制作病理切片，并在光學(xué)顯微鏡下觀察其變化，采用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)（RT-PCR）檢測(cè)造模后0.5h、1h、3h的TNF-αmRNA、IL-6mRNA表達(dá)水平的變化。 結(jié)果： 1、各組實(shí)驗(yàn)犬肛溫隨時(shí)間的變化實(shí)驗(yàn)組（B、C、D、E、F組）犬的肛溫在各時(shí)間點(diǎn)（10min、1h、2h、3h）均有升高，隨著時(shí)間的推移，肛溫逐漸升高，以F組對(duì)犬的肛溫影響最大，實(shí)驗(yàn)組分別與正常組比較，差異有統(tǒng)計(jì)學(xué)意義（PO.05）。通過(guò)實(shí)驗(yàn)，濕熱、高血糖、顱腦火器傷三重因素實(shí)驗(yàn)條件下的肛溫變化較其他兩兩組合的雙重實(shí)驗(yàn)條件更為劇烈，兩兩組合的雙重實(shí)驗(yàn)條件較單純因素的實(shí)驗(yàn)條件下肛溫的變化劇烈。 2、末端回腸組織肉眼及鏡下炎性評(píng)分肉眼觀，實(shí)驗(yàn)組（B、C、D、E、F組）均以急性炎性反應(yīng)為表現(xiàn)，且F組較其它組炎性反應(yīng)嚴(yán)重；病理切片鏡下示，實(shí)驗(yàn)組炎性細(xì)胞浸潤(rùn)增多，淋巴管、乳糜管擴(kuò)張，絨毛變粗變短，表現(xiàn)為急性炎性反應(yīng)，實(shí)驗(yàn)組分別與正常組比較，差異有統(tǒng)計(jì)學(xué)意義（PO.05）。各實(shí)驗(yàn)組出現(xiàn)炎性評(píng)分隨時(shí)間的延長(zhǎng)，其炎性損傷的程度逐漸加重。 3、末端回腸組織TNF-αmRNA的表達(dá)水平各實(shí)驗(yàn)組（B、C、D、E、F組）傷后0.5h、1h、3h，TNF-αmRNA在末端回腸組織的表達(dá)逐漸升高，以F組升高最大，實(shí)驗(yàn)組分別與正常組比較，差異有統(tǒng)計(jì)學(xué)意義（PO.05）。通過(guò)實(shí)驗(yàn)表明，TNF-α可能參與了實(shí)驗(yàn)性急性末端回腸炎的發(fā)病過(guò)程，與時(shí)間呈依賴性，隨著末端回腸組織炎性損傷的加重，其表達(dá)量增加，具有明顯促炎作用。 4、末端回腸組織IL-6mRNA的表達(dá)水平傷后0.5h、1h、3h，實(shí)驗(yàn)組（B、C、D、E、F組）IL-6mRNA在末端回腸組織的表達(dá)依次升高，且隨著時(shí)間延長(zhǎng)，升高數(shù)值越大，以F組最明顯，實(shí)驗(yàn)組分別與正常組比較，，差異有統(tǒng)計(jì)學(xué)意義（PO.05）。通過(guò)實(shí)驗(yàn)說(shuō)明，IL-6在實(shí)驗(yàn)性急性末端回腸炎的發(fā)病過(guò)程中與時(shí)間呈依賴性，但其整體表達(dá)水平均較TNF-αmRNA的少,IL-6可能在實(shí)驗(yàn)性急性末端回腸炎的發(fā)病過(guò)程中起著輔助促炎效應(yīng)。 結(jié)論： 1、在實(shí)驗(yàn)性ATI中，隨著時(shí)間的延長(zhǎng)，末端回腸組織炎性損傷逐漸加重； 2、TNF-α、IL-6在實(shí)驗(yàn)性ATI中的表達(dá)呈時(shí)間依賴性； 3、TNF-α、IL-6協(xié)同參與了實(shí)驗(yàn)性ATI的發(fā)病過(guò)程。
[Abstract]:Objective : To investigate the expression level of TNF - 偽 and IL - 6 in experimental acute terminal ileitis .

Methods : Thirty healthy adult male hybrid dogs were randomly divided into six groups : normal control group ( group A ) , high - temperature high - humidity hyperglycemia group ( group B ) , normal blood sugar craniocerebral gunshot wound group ( group C ) , high blood glucose and craniocerebral gunshot wound group ( group D ) , normal blood glucose wet - heat and craniocerebral gunshot wound group ( group F ) .

Results :

1 . The anal temperature of dogs in experimental group ( B , C , D , E , F ) increased with time ( 10min , 1h , 2h , 3h ) .

2 . At the end ileum , the visual and microscopic inflammatory scores were observed . The experimental groups ( group B , C , D , E , F ) were characterized by acute inflammatory reaction , and the F group had more inflammatory response than other groups .
Under the pathological section , the inflammatory cell infiltration in experimental group increased , the lymphatic vessels , the breast erosions were dilated and the villi became shorter and shorter . The experimental group showed acute inflammatory reaction . The experimental group had significant difference compared with the normal group ( PO.05 ) . The inflammatory score of each experimental group increased with time , and the degree of inflammatory injury was gradually increased .

3 . The expression level of TNF - 偽 mRNA in the terminal ileum was 0.5 h , 1 h , 3 h , and TNF - 偽 mRNA increased gradually in the terminal ileum . The results showed that TNF - 偽 might be involved in the pathogenesis of experimental acute terminal ileitis .

4 . IL - 6 mRNA expression in terminal ileum was 0.5h , 1h , 3h . The expression of IL - 6 mRNA in the terminal ileum was increased in the experimental group . The results showed that IL - 6 had a statistically significant difference with time in the pathogenesis of experimental acute terminal ileitis , but the expression level of IL - 6 was lower than that of normal group . IL - 6 could play an auxiliary pro - inflammatory effect in the pathogenesis of experimental acute terminal ileitis .

Conclusion :

1 . In the experimental ATI , with the extension of time , the inflammatory injury of terminal ileum was gradually increased ;


2 . The expression of TNF - 偽 and IL - 6 in experimental ATI was time - dependent ;


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