本文選題：肝硬化 + 癌　； 參考：《臨床肝膽病雜志》2017年09期

【摘要】：目的比較分析肝硬化和肝硬化基礎(chǔ)上發(fā)生肝細(xì)胞癌(HCC)的患者遠(yuǎn)端腸道菌群的差異,探討肝硬化患者發(fā)生HCC與腸道菌群變化的關(guān)系。方法收集2015年12月-2016年5月天津市第三中心醫(yī)院肝內(nèi)科住院的慢性肝病患者35例,包括20例肝硬化患者(肝硬化組)和15例肝硬化合并HCC患者(HCC組)。收集患者糞便標(biāo)本,提取其腸道細(xì)菌總DNA,采用Roche 454測(cè)序技術(shù)對(duì)16S r DNA V3-V6可變區(qū)測(cè)序,并進(jìn)行生物信息分析(物種分類、豐度分析及多樣性分析)。計(jì)量資料組間比較采用t檢驗(yàn),計(jì)數(shù)資料組間比較采用Mann-Whitney U檢驗(yàn)。結(jié)果 20個(gè)肝硬化患者樣品平均OTU為306.50±163.76,15個(gè)HCC患者樣品平均OTU為357.24±168.85,2組患者OTU及alpha多樣性指數(shù)相比,差異沒(méi)有統(tǒng)計(jì)學(xué)意義(P值均0.05)。糞便菌群序列包括的菌門(mén)主要有放線菌門(mén)(Actinobacteria)、擬桿菌門(mén)(Bacteroidetes)、厚壁菌門(mén)(Firmicutes)和變形菌門(mén)(Proteobacteria)。屬種定量及組成分析發(fā)現(xiàn),肝硬化組和HCC組患者腸道內(nèi)多種細(xì)菌的相對(duì)豐度差異顯著,其中肝硬化組腸道菌群與HCC組比較,放線菌門(mén)(0.21%vs 0.06%,U=89.000,P=0.043)、雙歧桿菌屬(0.16%vs 0.04%,U=90.000,P=0.046)和梭菌屬(0.13%vs 0.08%,U=90.000,P=0.046)所占比例顯著升高,理研菌科(0.58%vs 2.30%,U=82.000,P=0.023)和Christenellaceae科(0.01%vs0.08%,U=84.500,P=0.028)所占比例顯著降低。結(jié)論與單純肝硬化患者相比,肝硬化基礎(chǔ)上發(fā)生HCC者腸道菌群的組成有顯著差異,但這些差異與肝硬化基礎(chǔ)上發(fā)生HCC之間相互作用的具體機(jī)制尚不明確。
[Abstract]:Objective to investigate the relationship between HCC and intestinal flora in patients with cirrhosis and cirrhosis. Methods from December 2015 to May 2016, 35 patients with chronic liver disease, including 20 patients with liver cirrhosis and 15 patients with HCC, were enrolled in Department of Hepatology, third Center Hospital of Tianjin. The fecal samples of patients were collected and the total intestinal bacterial DNA was extracted. The 16s r DNA V3-V6 variable region was sequenced by Roche 454 sequencing technique, and the biological information was analyzed (species classification, abundance analysis and diversity analysis). T test was used to compare measurement data and Mann-Whitney U test was used to compare counting data. Results the average OTU of 20 patients with liver cirrhosis was 306.50 鹵163.76, and the average OTU of 15 patients with HCC was 357.24 鹵168.85. There was no significant difference in the diversity index of OTU and alpha between the two groups (P < 0.05). The sequence of faecal bacteria includes Actinobacteria, Bacteroidetesa, Firmicutesand Proteobacteria. Quantitative analysis and composition analysis showed that the relative abundance of many bacteria in the intestine of patients with cirrhosis and HCC was significantly different, and the intestinal flora of cirrhosis group was higher than that of HCC group. The proportions of 0.21 vs 0.06 U89.000 P0.043, 0.16 vs 0.04 000 of Bifidobacterium and 0.13% of Clostridium 0.13 vs 0.080.000 P0.046) increased significantly, and the percentages of 0.58 vs 2.30% of Rhizoma 82.000 P0.023) and 0.01vs0.084.500 (P0.028) decreased significantly. Conclusion there are significant differences in the composition of intestinal flora in patients with HCC on the basis of cirrhosis as compared with those in patients with cirrhosis alone, but the specific mechanism of the interaction between these differences and HCC on the basis of cirrhosis is not clear.
【作者單位】： 天津醫(yī)科大學(xué)三中心臨床學(xué)院天津市第三中心醫(yī)院肝內(nèi)科天津市人工細(xì)胞重點(diǎn)實(shí)驗(yàn)室天津市肝膽疾病研究所;天津市河?xùn)|區(qū)口腔醫(yī)院;
【基金】：國(guó)家科技重大專項(xiàng)(2016ZX10002008-07) 天津市科技計(jì)劃項(xiàng)目(13RCGFSY19200)
【分類號(hào)】：R575.2;R735.7
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本文編號(hào)：1885467