本文選題：便秘 + 血管活性腸肽　； 參考：《中南大學(xué)學(xué)報(醫(yī)學(xué)版)》2016年11期

【摘要】：目的:觀察血管活性腸肽(vasoactive intestinal peptide,VIP)對便秘大鼠腸道水液代謝、環(huán)磷酸腺苷-蛋白激酶A信號通路(cyclic AMP protein kinase A signaling pathway,c AMP-PKA)和水通道蛋白3(water channel protein 3,AQP3)的影響,探討VIP治療便秘的作用及機制。方法:45只健康成年Sprague-Dawley大鼠隨機分為空白對照組、模型組、模型+VIP組。給藥4周后,墨汁灌胃法檢測大鼠首粒黑便排出時間;根據(jù)大鼠糞便干濕重計算糞便含水率;HE染色觀察各組大鼠結(jié)腸組織形態(tài)學(xué)變化;Western印跡檢測各組大鼠結(jié)腸組織中VIP和AQP3蛋白表達水平;定量即時聚合酶鏈鎖反應(yīng)(quantitative real time polymerase chain reaction,q PCR)檢測各組大鼠結(jié)腸組織中c AMP,PKA和AQP3 m RNA的表達水平。結(jié)果:與空白對照組比較,模型組大鼠首粒黑便出現(xiàn)時間延長,糞便含水率明顯減少(均P0.01);結(jié)腸黏膜上皮部分破壞,杯狀細胞體積減小,數(shù)量明顯減少;結(jié)腸組織中VIP和AQP3蛋白含量明顯減少,AQP3,c AMP和PKA m RNA相對表達水平均有所降低(均P0.05)。與模型組比較,模型+VIP組大鼠首粒黑便出現(xiàn)時間縮短,糞便含水率明顯增加(均P0.05);結(jié)腸黏膜上皮完整性明顯改善,杯狀細胞體積增大,數(shù)量增多;結(jié)腸組織中VIP和AQP3蛋白含量增多,CAMP,PKA和AQP3 m RNA相對表達水平升高(均P0.05)。結(jié)論:VIP靜脈注射能夠調(diào)節(jié)腸道水液代謝,改善大鼠便秘癥狀,其機制可能與調(diào)節(jié)VIP-c AMP-PKA-AQP3信號通路有關(guān)。
[Abstract]:Aim: to investigate the effects of vasoactive intestinal peptide (VIPP) on intestinal water metabolism, cyclic AMP protein kinase A signaling pathwayc AMP-PKA and aquaporin 3(water channel protein 3 aquaporin (AQP3) in rats with constipation, and to explore the mechanism of VIP in the treatment of constipation. Methods 45 healthy adult Sprague-Dawley rats were randomly divided into control group, model group and model VIP group. After 4 weeks of administration, the time of excretion of the first black stool was detected by the method of Chinese ink gavage. The morphological changes of colonic tissue were observed by HE staining. The expression of VIP and AQP3 protein in colonic tissue of rats in each group was detected by Western blot. Quantitative real time polymerase chain reactionQ PCR was used to detect the expression of c-AMPP-PKA and AQP3 m RNA in colonic tissues of rats in each group. Results: compared with the blank control group, the appearance time of the first black stool in the model group was prolonged, and the fecal moisture content was significantly decreased (all P 0.01), the colonic mucosal epithelium was partially destroyed, the goblet cell volume was decreased and the number of goblet cells was significantly decreased. The protein content of VIP and AQP3 in colonic tissue was significantly decreased, and the relative expression of AQP3C AMP and PKA m RNA were all decreased (P0.05). Compared with the model group, the appearance time of the first black stool was shortened and the fecal moisture content was significantly increased in the model VIP group (all P 0.05), the integrity of colonic mucosal epithelium was improved significantly, the goblet cell volume was increased and the number of goblet cells was increased. The protein content of VIP and AQP3 increased and the relative expression of AQP3 m RNA and PKA increased in colon tissue (P0.05). Conclusion the control of intestinal fluid metabolism and the improvement of constipation symptoms may be related to the regulation of VIP-c AMP-PKA-AQP3 signaling pathway.
【作者單位】： 陜西中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院;陜西中醫(yī)藥大學(xué)人事處;陜西中醫(yī)藥大學(xué)協(xié)同創(chuàng)新中心;
【基金】：國家自然科學(xué)基金(81273663) 陜西省教育廳項目(320104-203010021)~~
【分類號】：R574.62

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