本文選題：酒精性肝損傷 + Lierber-De　； 參考：《中國比較醫(yī)學(xué)雜志》2016年06期

【摘要】：目的篩選一種簡(jiǎn)便、穩(wěn)定、可靠的酒精性肝損傷模型。方法通過酒精灌胃或給予LierberDe Carli酒精液體飼料8周來建立酒精性肝損傷小鼠模型。實(shí)驗(yàn)期間記錄小鼠精神狀態(tài)、攝食量和體重變化,HE染色進(jìn)行病理學(xué)分析,分析血清谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、γ-谷氨酰轉(zhuǎn)移酶(γ-GT)、堿性磷酸酶(AKP)活性,血清和肝臟總膽固醇(TC)、甘油三酯(TG)含量等肝損傷指標(biāo)。結(jié)果造模8周后,兩種方式均導(dǎo)致小鼠肝臟脂質(zhì)聚集等組織病理學(xué)變化,血清ALT、AST、AKP活性、血清和肝臟TG含量均顯著提高,提示出現(xiàn)明顯肝損傷。酒精灌胃導(dǎo)致小鼠精神狀態(tài)差,并顯著減輕體重,而酒精液體飼料對(duì)小鼠精神狀態(tài)和體重影響小。酒精液體飼料模型肝臟指數(shù)和血清TC水平顯著增加,而且血清ALT、AST活性、血清和肝臟TG含量及肝臟脂質(zhì)聚集等組織病理學(xué)變化幅度大于酒精灌胃模型,提示前者的肝損傷程度比后者嚴(yán)重。結(jié)論 Lieber-De Carli酒精液體飼料模型優(yōu)于酒精灌胃模型。Lieber-De Carli酒精液體飼料模型相對(duì)酒精灌胃模型更適合用于酒精性肝損傷分子機(jī)制的研究和防治藥物的篩選。
[Abstract]:Objective to screen a simple, stable and reliable alcoholic liver injury model. Methods the model of alcoholic liver injury was established by oral administration of alcohol or LierberDe Carli alcohol liquid feed for 8 weeks. During the experiment, the changes of mental state, food intake and body weight of mice were recorded for pathological analysis by HE staining, and the activities of serum alanine aminotransferase (alt), aspartate aminotransferase (AST), 緯 -glutamyl transferase (緯 -GT), alkaline phosphatase (ALP) were analyzed. Serum and liver total cholesterol (TC), triglyceride (TG) content and other liver injury indexes. Results after 8 weeks of modeling, both of the two methods resulted in histopathological changes such as lipid aggregation in the liver of mice. The activity of AKP in serum of alt ASTN and the content of TG in serum and liver were significantly increased, indicating that there was obvious liver injury. Alcohol administration resulted in poor mental state and significant weight loss in mice, while alcohol liquid diet had little effect on mental state and body weight of mice. The liver index and serum TC level of alcohol liquid feed model were significantly increased, and the activity of alt AST, TG content of serum and liver, liver lipid aggregation and other histopathological changes were larger than those of alcohol gastric perfusion model. These results suggest that the liver injury of the former is more serious than that of the latter. Conclusion the Lieber-De Carli alcohol liquid feed model is better than the alcohol perfusion model. Lieber-De Carli alcohol feed model is more suitable for the study of the molecular mechanism of alcoholic liver injury and the screening of preventive and therapeutic drugs.
【作者單位】： 華南農(nóng)業(yè)大學(xué)食品學(xué)院;廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所/農(nóng)業(yè)部功能食品重點(diǎn)實(shí)驗(yàn)室/廣東省農(nóng)產(chǎn)品加工重點(diǎn)實(shí)驗(yàn)室;深圳職業(yè)技術(shù)學(xué)院;
【基金】：國家自然科學(xué)基金-青年科學(xué)基金項(xiàng)目(NSFC 31501478) 廣東省自然科學(xué)基金博士啟動(dòng)項(xiàng)目(2014A030310328) 深圳市科技計(jì)劃基礎(chǔ)研究項(xiàng)目(JCYJ20140901162541286) 廣東省省級(jí)科技計(jì)劃項(xiàng)目(2016B070701012)
【分類號(hào)】：R575;R-332

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