本文選題：螺桿菌 + 幽門��； 參考：《重慶醫(yī)學(xué)》2015年03期

【摘要】：目的研究幽門螺旋桿菌(簡稱幽門螺桿菌)感染小鼠胃黏膜組織細(xì)胞因子變化,初步探討幽門螺桿菌感染的免疫機(jī)制。方法選取8~10周齡BALB/c小鼠24只,雌雄各半,分為觀察組和對(duì)照組,兩組造模前均禁食12h,觀察組以2×109CFU/mL濃度的幽門螺桿菌菌液0.5mL/d灌胃,連續(xù)7d。對(duì)照組灌胃給生理鹽水,0.5mL/d,連續(xù)7d,進(jìn)行幽門螺桿菌感染小鼠胃黏膜造模。采用實(shí)時(shí)定量熒光PCR(Real-time PCR)檢測檢測感染2、4周后,小鼠血漿中γ干擾素(IFN-γ)、重組改構(gòu)人腫瘤壞死因子α(TNF-α)、白細(xì)胞介素-8(IL-8)以及IL-10mRNA的表達(dá)量,并于感染4周后,處死小鼠,無菌取各組小鼠的胃組織,采用酶聯(lián)免疫吸附試驗(yàn)夾心法檢測胃黏膜組織中IFN-γ、TNF-α、IL-8及IL-10蛋白的水平。結(jié)果兩組小鼠感染2周后,觀察組IL-8高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。感染4周后,IFN-γ、TNF-α、IL-8的mRNA及蛋白表達(dá)量均明顯上升,但觀察組上升幅度明顯高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論TH2細(xì)胞在幽門螺桿菌感染中的作用表現(xiàn)不明顯,能促進(jìn)TH1型細(xì)胞細(xì)胞因子的表達(dá),引發(fā)TH1為主的免疫反應(yīng)。
[Abstract]:Objective to study the changes of cytokines in gastric mucosa of mice infected with Helicobacter pylori (Helicobacter pylori) and to explore the immune mechanism of Helicobacter pylori infection. Methods 24 10-week-old BALB/c mice, half male and half female, were divided into two groups: the observation group and the control group. The two groups fasted for 12 hours before modeling. The observation group was fed with 2 脳 109CFU/mL Helicobacter pylori solution 0.5mL/d for 7 days. The control group was given normal saline 0.5 mL / d for 7 days to model the gastric mucosa of mice infected with Helicobacter pylori. The expression of IFN- 緯, recombinant human tumor necrosis factor- 偽 (TNF- 偽), interleukin-8 (IL-8) and IL-10mRNA in plasma of mice were detected by real-time fluorescence PCR(Real-time 4 weeks after infection. The mice were killed after 4 weeks of infection. The gastric tissues of each group were collected and the levels of IFN- 緯 TNF- 偽 TNF- 偽 IL-8 and IL-10 protein in gastric mucosa were detected by enzyme-linked immunosorbent assay (Elisa). Results after 2 weeks of infection, the IL-8 in the observation group was higher than that in the control group, and the difference was statistically significant (P 0.05). After 4 weeks of infection, the expression of mRNA and protein of TNF- 偽 TNF- 偽 IL-8 in the observation group was significantly higher than that in the control group, and the difference was statistically significant (P 0.05). Conclusion the effect of TH2 cells on Helicobacter pylori infection is not obvious, which can promote the expression of TH1 cytokines and induce the immune response of TH1.
【作者單位】： 新疆石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院消化內(nèi)科;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(49201065)
【分類號(hào)】：R573

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 熊瑛;陳嘉熠;吳麗虹;張毅;;幽門螺桿菌感染與非甾體抗炎藥對(duì)消化性潰瘍發(fā)病的影響[J];重慶醫(yī)學(xué);2012年26期

2 戚紅霞;李曉虹;袁亞;;胃癌組織cagA陽性幽門螺桿菌感染與環(huán)氧化酶-2的關(guān)系[J];重慶醫(yī)學(xué);2013年26期

3 陳秀英;沈洲立;魯厚勝;;幽門螺桿菌感染與胃食管反流病病程相關(guān)性研究[J];重慶醫(yī)學(xué);2014年08期

4 Wataru Shibata;Yoshihiro Hirata;Haruhiko Yoshida;Motoyuki Otsuka;Yujin Hoshida;Keiji Ogura;Shin Maeda;Tomoya Ohmae;Ayako Yanai;Yuzo Mitsuno;Naohiko Seki;Takao Kawabe;Masao Omata;;NF-κB and ERK-signaling pathways contribute to the gene expression induced by cag PAI-positive-Helicobacter pylori infection[J];World Journal of Gastroenterology;2005年39期

5 Kawiya Sintara;Duangporn Thong-Ngam;Suthiluk Patumraj;Naruemon Klaikeaw;Tanittha Chatsuwan;;Curcumin suppresses gastric NF-κB activation and macromolecular leakage in Helicobacter pylori-infected rats[J];World Journal of Gastroenterology;2010年32期

6 Isabel Adler;Andrea Mui濼o;Silvia Aguas;Laura Harada;Mariana Diaz;Adriana Lence;Mario Labbrozzi;Juan Manuel Mui濼o;Boris Elsner;Alejandra Avagnina;Valeria Denninghoff;;Helicobacter pylori and oral pathology:Relationship with the gastric infection[J];World Journal of Gastroenterology;2014年29期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李雅琳;王啟之;;姜黃素在消化系統(tǒng)疾病治療中的新進(jìn)展[J];國際消化病雜志;2011年06期

2 蔣正舉;劉兆輝;張春林;劉華慶;吳興龍;楊永福;;NF-κB、c-myc在成人喉乳頭狀瘤中的表達(dá)及意義[J];貴州醫(yī)藥;2012年08期

3 黃澤艷;;護(hù)理干預(yù)對(duì)消化性潰瘍患者56例的療效觀察[J];貴陽中醫(yī)學(xué)院學(xué)報(bào);2013年02期

4 尹川;唐世孝;;姜黃素抗胃癌機(jī)制研究進(jìn)展[J];國際消化病雜志;2014年01期

5 范仰鋼;;NSAIDs對(duì)伴有HP感染風(fēng)濕病患者上消化道損害的危險(xiǎn)性探究[J];重慶醫(yī)學(xué);2014年31期

6 況榮華;周林;傅穎s，

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