本文選題：乙型肝炎病毒 + 單核苷酸多態(tài)性��； 參考：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：【目的】探討HLA-DP、DQ基因單核苷酸多態(tài)性與福建漢族人群HBV感染及其不同結(jié)局的關(guān)系。 【方法】采用PCR-SSP基因分型的方法對(duì)健康人群（n=204）、HBV自限性感染人群（n=255）、慢性HBV感染人群（無(wú)癥狀攜帶者204例、慢性乙型肝炎136例、肝硬化及肝細(xì)胞癌各68例）進(jìn)行rs3077、rs9277535、rs2647050位點(diǎn)的多態(tài)性檢測(cè)。 【結(jié)果】健康對(duì)照組、自限性感染組和無(wú)癥狀HBV攜帶者組3個(gè)SNP位點(diǎn)基因型均符合Hardy-Weinberg遺傳平衡。健康對(duì)照組、自限性感染組與慢性感染組相比，二元Logistic回歸分析校正性別與年齡影響因素后，，rs3077、rs9277535位點(diǎn)A等位基因在顯性和加性模型下均有統(tǒng)計(jì)學(xué)意義（P0.05），而rs2647050位點(diǎn)A等位基因在顯性和加性模型無(wú)統(tǒng)計(jì)學(xué)意義（P0.05），單倍型GGA、AGA、AAA與HBV感染存在相關(guān)性（P0.05）。以無(wú)癥狀攜帶者為對(duì)照組，分別與慢性乙型肝炎組、肝硬化組及肝細(xì)胞癌組相比，以上3個(gè)SNP與HBV感染結(jié)局均無(wú)統(tǒng)計(jì)關(guān)聯(lián)（P0.05），也無(wú)單倍型與HBV感染結(jié)局存在關(guān)聯(lián)（P0.05）。 【結(jié)論】HLA-DP基因多態(tài)性與慢性HBV感染存在相關(guān)性，rs3077A、rs9277535A是慢性HBV感染的保護(hù)因素。HLA-DP、DQ基因單核苷酸多態(tài)性與HBV感染不同結(jié)局之間無(wú)相關(guān)性。 【目的】綜合評(píng)價(jià)HLA-DP基因多態(tài)性與中國(guó)人群患肝癌風(fēng)險(xiǎn)的相關(guān)性。 【方法】系統(tǒng)檢索PubMed、FMJS、CNKI及萬(wàn)方數(shù)據(jù)庫(kù)有關(guān)HLA-DP基因多態(tài)性與肝癌相關(guān)性的病例對(duì)照研究，按納入標(biāo)準(zhǔn)選擇文獻(xiàn)并提取相關(guān)信息，應(yīng)用Stata12.0軟件進(jìn)行Meta分析。 【結(jié)果】本研究共納入HLA-DP基因多態(tài)性與HBV相關(guān)肝癌相關(guān)文獻(xiàn)3篇。Meta分析結(jié)果顯示，rs3077A在顯性模型下可降低患肝癌的風(fēng)險(xiǎn)（AA+AG vs GG：OR=0.83，95%CI：0.74～0.93），而rs9277535與患肝癌風(fēng)險(xiǎn)無(wú)相關(guān)性（AA+AGvs GG：OR=0.95，95%CI：0.85～1.06）。 【結(jié)論】通過(guò)Meta分析，本研究發(fā)現(xiàn)HLA-DPA1基因rs3077A在顯性模型下可降低患肝癌的風(fēng)險(xiǎn)。
[Abstract]:[objective] to investigate the relationship between HLA-DPnDQ gene single nucleotide polymorphism (SNP) and HBV infection and its different outcomes in Fujian Han population.[methods] PCR-SSP genotyping was used to study the prevalence of PCR-SSP infection in healthy persons (204 asymptomatic carriers, 136 chronic hepatitis B), and in patients with chronic HBV infection (204 asymptomatic carriers and 136 chronic hepatitis B).The polymorphism of rs3077 rs9277535rs2647050 was detected in 68 cases of liver cirrhosis and 68 cases of hepatocellular carcinoma respectively.[results] three SNP locus genotypes in healthy control group, self limited infection group and asymptomatic HBV carrier group all accord with Hardy-Weinberg genetic balance.In the healthy control group, the self-limited infection group was compared with the chronic infection group,A allele at rs3077 rs9277535 was statistically significant in both dominant and additive models by binary Logistic regression analysis, while the rs2647050 locus A allele had no statistical significance in dominant and additive models.There was a correlation between AGAA A and HBV infection (P0.05A).Compared with chronic hepatitis B group, liver cirrhosis group and hepatocellular carcinoma group, there was no statistical correlation between SNP and HBV infection outcome, and no haplotype was associated with HBV infection outcome.[conclusion] there is a correlation between the polymorphism of HLA-DP gene and chronic HBV infection. There is no correlation between the single nucleotide polymorphisms of HLA-DPnDQ gene and the different outcomes of HBV infection, rs3077A rs9277535A is the protective factor of chronic HBV infection.[objective] to evaluate the association of HLA-DP gene polymorphism with the risk of liver cancer in Chinese population.[methods] A case-control study on the association between HLA-DP gene polymorphism and hepatocellular carcinoma was carried out in PubMedus FMJSN CNKI and Wanfang database. According to the inclusion criteria, literature was selected and relevant information was extracted. Meta analysis was carried out with Stata12.0 software.[results] A total of 3 references related to HLA-DP gene polymorphisms and HBV related hepatocellular carcinoma were included in this study. The results of meta-analysis showed that rs3077A could reduce the risk of liver cancer under dominant model and reduce the risk of HCC by AA AG vs GG: OR0.8395CIW 0.7495 CIW 0.740.93, but rs9277535 had no correlation with the risk of HCC.[conclusion] by Meta analysis, we found that HLA-DPA1 gene rs3077A can reduce the risk of liver cancer in dominant model.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R512.62

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 ;Influence of HLA-DRB1 alleles and HBV genotypes on interferon-α therapy for chronic hepatitis B[J];World Journal of Gastroenterology;2005年30期

2 ;Relationship of human leukocyte antigen class II genes with the susceptibility to hepatitis B virus infection and the response to interferon in HBV-infected patients[J];World Journal of Gastroenterology;2005年36期

本文編號(hào)：1750521