發(fā)布時(shí)間：2018-04-04 06:20

  本文選題：潰瘍性結(jié)腸炎(UC)　切入點(diǎn)：白細(xì)胞介素-β　出處：《中國老年學(xué)雜志》2015年02期

【摘要】：目的探討潰瘍性結(jié)腸炎(UC)大鼠血清白細(xì)胞介素-1β(IL-1β)、腫瘤壞死因子-α(TNF-α)、IL-4的動(dòng)態(tài)表達(dá)規(guī)律。方法將60只大鼠隨機(jī)分為空白組10只、乙醇組10只,模型組40只(3、7、14、21 d各10只),采用TNBS/乙醇灌腸法制備潰瘍性結(jié)腸炎大鼠模型,于制模后3、7、14、21 d 4個(gè)時(shí)間點(diǎn)采用ELISA法檢測大鼠血清IL-1β、TNF-α、IL-4水平,光鏡觀察結(jié)腸組織形態(tài)并評分。結(jié)果制模后3 d大鼠結(jié)腸出現(xiàn)炎癥和潰瘍,且隨著時(shí)間推移而加重,7~14 d潰瘍和炎癥最為嚴(yán)重,21 d潰瘍和炎癥已經(jīng)有所修復(fù)。模型組各時(shí)間點(diǎn)大鼠血清促炎因子IL-1β、TNF-α均高于空白組和乙醇組,抗炎因子IL-4水平均低于空白組和乙醇組,以7 d和14 d升高或降低更為顯著(P0.05、P0.01);7、14 d組與3d組比較,IL-1β、TNF-α水平進(jìn)一步升高(P0.01),而IL-4水平進(jìn)一步降低(P0.01);21 d組與7、14 d組比較,IL-1β、TNF-α水平下降,而IL-4水平則升高(P0.05)。結(jié)論促炎因子IL-1β、TNF-α和抗炎因子IL-4在UC發(fā)病中起重要作用,UC的發(fā)病及嚴(yán)重程度與促炎因子IL-1β、TNF-α、IL-4的動(dòng)態(tài)表達(dá)及失衡密切相關(guān)。
[Abstract]:Objective to investigate the dynamic expression of IL-4 in serum of rats with ulcerative colitis (UC) and tumor necrosis factor- 偽 (TNF- 偽).Methods 60 rats were randomly divided into blank group (n = 10), ethanol group (n = 10) and model group (n = 40). The ulcerative colitis model was established by TNBS/ ethanol enema.The serum levels of TNF- 偽 IL-4 in serum of rats were measured by ELISA method at 4 time points of 21 days after 3 days, 7 days and 14 days, and the colonic tissue morphology was observed by light microscope and evaluated.Results the colonic inflammation and ulceration appeared in rats 3 days after the model making, and the ulcer and inflammation were aggravated at 714 days and the most serious ulcer and inflammation were repaired in 21 days.The serum levels of IL-1 尾 -TNF- 偽 in model group were higher than those in control group and ethanol group, and the level of anti-inflammatory factor IL-4 in model group was lower than that in blank group and ethanol group.浠，

本文編號：1708736