本文選題：蝦青素　切入點：蛋氨酸-膽堿缺乏飲食　出處：《現(xiàn)代預防醫(yī)學》2016年04期 　論文類型：期刊論文

【摘要】：目的通過蛋氨酸-膽堿缺乏(Methionine-Choline-Deficient,MCD)飲食誘導的小鼠脂肪性肝炎(NonAlcoholic Steatohepatitis,NASH)模型研究蝦青素抗非酒精性脂肪肝纖維化效應及其機制。方法將30只雄性C57BL/6J小鼠隨機分為對照組、模型組和蝦青素組,每組10只。模型組飼喂MCD飼料,對照組在MCD飼料基礎上每kg飼料添加3 g L-蛋氨酸和2 g膽堿;蝦青素組在MCD飼料基礎上用蝦青素口服液灌胃(20 mg/kg.bw),每周2次。4周后,稱量各組小鼠體重、肝重并計算肝臟指數(shù);測定小鼠血清TG、TC、ALT、AST及肝臟TG、TC水平。肝組織蘇木素和伊紅(Hematoxylin and Eosin,HE)染色觀察組織形態(tài)學變化;熒光定量PCR檢測α-平滑肌肌動蛋白(α-Smooth Muscle Actin,α-SMA)和Ⅰ型膠原的m RNA表達水平。結果模型組小鼠血清ALT、AST水平,肝臟TG、TC水平及α-SMA和Ⅰ型膠原的m RNA表達與對照組相比顯著升高(P0.05),上述指標蝦青素組與模型組相比顯著降低(P0.05);模型組和蝦青素組小鼠血清TG、TC水平與對照組相比顯著降低(P0.05)。HE染色結果表明,模型組肝組織可見大量脂肪變性,而蝦青素組脂肪變性程度明顯減輕。結論蝦青素可以改善肝功能,且可能通過調控α-SMA和Ⅰ型膠原基因的表達減緩MCD飲食誘導的非酒精性脂肪肝纖維化進程。
[Abstract]:Objective to study the effect of astaxanthin on non-alcoholic fatty liver fibrosis induced by methionine-Choline-Deficientine-MCD-induced fatty hepatitis in mice and its mechanism. Methods Thirty male C57BL / 6J mice were randomly divided into control group. Model group and astaxanthin group, 10 rats in each group. The model group was fed with MCD diet, while the control group was fed with 3 g L-methionine and 2 g choline per kg diet on the basis of MCD diet. Astaxanthin group was fed with astaxanthin oral liquid on the basis of MCD feed. After 4 weeks twice a week, the weight and liver weight of each group were measured and the liver index was calculated. The levels of serum TGN, TGN, TC in liver, hematoxylin and Hematoxylin and Eosinhedron in liver tissue were determined. The histomorphologic changes were observed. The expression of 偽 -Smooth Muscle actin (偽 -SMAA) and collagen 鈪，

本文編號：1635611