本文選題：白介素-32　切入點(diǎn)：C反應(yīng)蛋白　出處：《青海大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：通過測(cè)定慢性淺表性胃炎、慢性萎縮性胃炎、癌前病變、胃癌患者血清中CRP、IL-32濃度，探討二者與上述疾病的關(guān)系，尋找有助于早期診斷胃癌的血清學(xué)依據(jù)。 方法收集2013年2月－2013年12月因消化道癥狀前來青海大學(xué)附屬醫(yī)院消化科住院診治患者131例，慢性淺表性胃炎（對(duì)照組）27例、慢性萎縮性胃炎38例、癌前病變31例以及胃癌35例，并行14C呼氣試驗(yàn)判定Hp感染情況。采用散射比濁法測(cè)定CRP水平，，酶聯(lián)免疫吸附法（ELISA）檢測(cè)IL-32的水平，并進(jìn)行比較分析。 結(jié)果：1.胃癌組血清CRP和IL-32水平高于癌前病變組、萎縮性胃炎組和對(duì)照組，(P＜0.05)，癌前病變組血清CRP和IL-32水平高于萎縮性胃炎組和對(duì)照組(P＜0.05)，而萎縮性胃炎組血清CRP、IL-32與對(duì)照組相比無明顯差異(P＞0.05)。2.在癌前病變組和胃癌組中，Hp（+）者血清CRP和IL-32水平高于Hp（-）者，差異有統(tǒng)計(jì)學(xué)意義(P＜0.05)；而在對(duì)照組和慢性萎縮性胃炎組中，Hp（+）者血清CRP、IL-32水平與HP（-）者比較差異無統(tǒng)計(jì)學(xué)意義(P＞0.05)。3.癌前病變組血清IL-32與CRP是正相關(guān)，r=0.615，P＜0.05；胃癌組血清IL-32、CRP是正相關(guān)，r=0.775，P＜0.05。 結(jié)論：1.癌前病變組和胃癌組患者血清CRP和IL-32濃度明顯高于萎縮性胃炎組和對(duì)照組，且胃癌組高于癌前病變組，提示胃粘膜病變較重時(shí)，炎癥相關(guān)因子CRP和促炎因子IL-32表達(dá)活躍，有望作為胃癌的早期預(yù)警指標(biāo)。2.在癌前病變組和胃癌組中，Hp（+）者血清CRP和IL-32水平均高于Hp（-）者，而在對(duì)照組和萎縮性胃炎組中，Hp（+）者與Hp（-）者無明顯差異，可能提示Hp感染初期機(jī)體產(chǎn)生的抗炎因子可阻止炎癥反應(yīng)過度，若炎癥持續(xù)存在，抗炎因子無法阻止炎癥的侵襲，最終誘發(fā)并促進(jìn)胃癌的發(fā)生和擴(kuò)散。3. IL-32和CRP在癌前病變組和胃癌組中均具有明顯的正相關(guān)， IL-32可促進(jìn)細(xì)胞因子釋放，從而刺激肝細(xì)胞分泌并釋放CRP，推測(cè)IL-32與CRP是胃癌發(fā)生過程中的主要炎性介質(zhì)。
[Abstract]:Objective: To explore the relationship between the two factors and the above diseases by determining serum CRP and IL-32 concentrations in patients with chronic superficial gastritis, chronic atrophic gastritis, precancerous lesions and gastric cancer, and to find serological evidence for early diagnosis of gastric cancer.
Methods from February 2013 to December 2013 due to gastrointestinal symptoms of digestive department of Affiliated Hospital of Qinghai University hospital diagnosis and treatment of 131 cases of patients with chronic superficial gastritis (control group) 27 cases, 38 cases of chronic atrophic gastritis, 31 cases of precancerous lesion and 35 cases of gastric cancer, parallel 14C breath test to determine Hp infection. The level of CRP was measured by nephelometry methods enzyme linked immunosorbent assay (ELISA) to detect the level of IL-32, and comparative analysis.
Results: 1. gastric cancer group serum CRP and IL-32 level is higher than that of precancerous lesions of chronic atrophic gastritis group and control group (P < 0.05), precancerous lesion group serum CRP and IL-32 levels were significantly higher in atrophic gastritis group and control group (P < 0.05), and atrophic gastritis group serum CRP, IL-32 and control group showed no significant difference (P > 0.05).2. in precancerous lesions and gastric cancer group, Hp (+) CRP and serum level of IL-32 was higher than that of Hp (-), the difference was statistically significant (P < 0.05); while in the control group and chronic atrophic gastritis group, Hp (+) serum CRP, IL-32 level and HP (-) there was no significant difference (P > 0.05).3. precancerous lesion group serum IL-32 and CRP is positively related to r=0.615, P < 0.05; gastric cancer group serum IL-32, CRP is positively related to r=0.775, P < 0.05.
Conclusion: 1. precancerous lesions and gastric cancer patients serum CRP and IL-32 concentrations were significantly higher than those in atrophic gastritis group and control group, and the gastric cancer group was higher than that of precancerous lesions of gastric mucosal lesions that are heavier, inflammation related factors CRP and proinflammatory cytokine expression of IL-32 active, is expected as early warning indicators of.2. in gastric cancer precancerous lesions and gastric cancer group, Hp (+) CRP and serum IL-32 levels were significantly higher than that of Hp (-), while in the control group and atrophic gastritis group, Hp (+) and Hp (-) have no obvious difference, may suggest anti-inflammatory factor Hp early infection of the body to produce can to prevent excessive inflammation, if inflammation persists, anti-inflammatory factor cannot prevent inflammation and promote gastric cancer invasion, induce the occurrence and spread of.3., IL-32 and CRP in precancerous lesions and gastric cancer patients have obvious positive correlation, IL-32 can promote the release of cytokines, which stimulate liver cells CRP is secreted and released, and it is presumed that IL-32 and CRP are the main inflammatory mediators in the process of gastric cancer.

【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R573.3;R735.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 陳清波;王洪波;徐明W

本文編號(hào)：1586415