本文關鍵詞： 抗人類原肌球蛋白亞型5（hTM5） 抗中性粒細胞胞漿抗體（ANCA） 潰瘍性結腸炎（UC）　出處：《山西醫(yī)科大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的 通過檢測UC患者血清hTM5、ANCA與腸粘膜hTM5的表達，探討hTM5在UC中的臨床意義。 材料和方法 收集2012年4月至2013年6月山西醫(yī)科大學第一臨床醫(yī)院消化科門診和住院UC患者60例的血清、腸粘膜標本及臨床資料，其中UC中重度組30例，UC輕度組30例，診斷符合2012廣州會議“對我國IBD診斷治療規(guī)范的共識意見”[1]。采用ELISA方法檢測外周血中原肌球蛋白亞型5抗體含量，對照組為健康志愿者30例。結腸粘膜組織行免疫組織化學方法檢測腸粘膜原肌球蛋白亞型5陽性細胞數(shù)，對照組來自結腸癌行外科手術切除的正常結腸斷端。此外,還應用間接免疫熒光法檢測UC患者60例及健康對照組30例的血清中抗中性粒細胞胞漿抗體的陽性率。 結果 （1）免疫組織化學法檢測結果：中重度UC患者、輕度UC患者、健康對照人群結腸上皮細胞中原肌球蛋白亞型5陽性表達率差異有統(tǒng)計學意義(P＜0.05)。（2）酶聯(lián)免疫吸附法檢測結果：中重度UC患者、輕度UC患者、健康對照人群血清中原肌球蛋白亞型5抗體濃度差異有統(tǒng)計學意義(P＜0.05)。（3）間接免疫熒光法檢測結果：UC患者血清ANCA陽性率明顯高于健康對照組，差異有統(tǒng)計學意義（p＜0.05）。（4）血清hTM5濃度和ANCA陽性率做Spearman等級相關分析，發(fā)現(xiàn)血清hTM5濃度與ANCA陽性率呈正相關（r=0.428.p=0.000）。 結論 （1）UC患者結腸粘膜及血清中原肌球蛋白亞型5抗體的表達程度與UC病情輕重程度均呈正相關，提示原肌球蛋白亞型5影響UC的發(fā)病及嚴重程度。 （2）聯(lián)合檢測血清原肌球蛋白亞型5和抗中性粒細胞胞漿抗體有助于對UC的診斷與嚴重程度的判斷。
[Abstract]:Purpose. To investigate the clinical significance of hTM5 in UC by detecting the expression of hTM5 ANCA and intestinal mucosal hTM5 in patients with UC. Materials and methods. From April 2012 to June 2013, 60 patients with UC were collected from the Department of Digestive Medicine of the first Clinical Hospital of Shanxi Medical University, including 30 cases of moderate and severe UC and 30 cases of mild UC. The diagnosis was in accordance with the "consensus opinion on the criteria for diagnosis and treatment of IBD in China" [1]. ELISA method was used to detect the level of proto-myosin subtype 5 antibody in peripheral blood. The number of positive cells of myosin subtype 5 in intestinal mucosa was detected by immunohistochemical method in 30 healthy volunteers, and the control group came from the normal cut end of colon cancer surgically resected. The positive rates of anti-neutrophil cytoplasmic antibodies in 60 UC patients and 30 healthy controls were also detected by indirect immunofluorescence assay. Results. Immunohistochemical results: moderate and severe UC patients, mild UC patients, The positive rate of myosin subtype 5 in colonic epithelial cells of healthy controls was significantly different (P < 0.05). The results of enzyme-linked immunosorbent assay (Elisa) were as follows: moderate and severe UC patients, mild UC patients, and mild UC patients. The results of indirect immunofluorescence assay showed that the positive rate of serum ANCA in patients with 20% UC was significantly higher than that in healthy controls. The difference was statistically significant (P < 0.05) the serum hTM5 concentration and the positive rate of ANCA were analyzed by Spearman grade correlation analysis. It was found that the serum hTM5 concentration was positively correlated with the positive rate of ANCA. Conclusion. The expression of myosin subtype 5 antibody in colonic mucosa and serum was positively correlated with the severity of UC, suggesting that myosin subtype 5 affected the incidence and severity of UC. (2) combined detection of promyosin subtype 5 and anti-neutrophil cytoplasmic antibody is helpful to the diagnosis and severity of UC.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R574.62

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