發(fā)布時(shí)間：2018-02-22 05:54

  本文關(guān)鍵詞： 過氧化氫(HO) 氧化損傷 人肝癌細(xì)胞株(Hep G) 正常肝細(xì)胞株(Chang liver)　出處：《中國公共衛(wèi)生》2015年03期 　論文類型：期刊論文

【摘要】：目的比較過氧化氫誘導(dǎo)人肝癌細(xì)胞株(Hep G2)與正常肝細(xì)胞株(Chang liver)建立的肝細(xì)胞氧化應(yīng)激損傷模型。方法用不同濃度過氧化氫誘導(dǎo)Hep G2細(xì)胞和Chang liver細(xì)胞氧化應(yīng)激損傷,采用噻唑藍(lán)法檢測細(xì)胞生長抑制率;分光光度法測定培養(yǎng)液中乳酸脫氫酶(LDH)、谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)活性,以及肝細(xì)胞中超氧化物歧化酶(SOD)活性、還原型谷胱甘肽(GSH)及丙二醛(MDA)含量。結(jié)果作用時(shí)間在0.5~4 h時(shí),在75~600μmol/L濃度范圍內(nèi),過氧化氫可濃度和時(shí)間依賴性地抑制2種肝細(xì)胞增殖,促使細(xì)胞內(nèi)AST、ALT和LDH向培養(yǎng)液中釋放;細(xì)胞中SOD和GSH活性明顯降低,MDA含量明顯升高,表明2種肝細(xì)胞氧化損傷模型構(gòu)建成功;選擇300μmol/L H2O2作用4 h為致Hep G2和Chang liver細(xì)胞氧化應(yīng)激損傷的最佳條件,結(jié)果顯示,Hep G2和Chang liver細(xì)胞的生長抑制率分別為62%和76%,培養(yǎng)液中ALT、AST、LDH活性分別為(18.2±0.2)、(34.2±4.6)、(544.2±26.8)和(19.1±0.1)、(30.3±2.5)、(536.8±22.3)U/L,細(xì)胞中MDA含量分別為(7.8±0.9)和(8.6±1.1)nmol/mgprot。結(jié)論Hep G2與Chang liver細(xì)胞均可用于氧化應(yīng)激細(xì)胞損傷模型的制備。
[Abstract]:Objective to compare the oxidative stress injury model of Hep G2 cells and Chang liver cells induced by hydrogen peroxide (Hep G 2) and normal liver cell line Chang liver.Methods the oxidative stress injury of Hep G2 cells and Chang liver cells was induced by hydrogen peroxide. The cell growth inhibition rate was measured by thiazolyl blue assay, and the activities of lactate dehydrogenase (LDH), alanine aminotransferase (alt), aspartate aminotransferase (AST), and superoxide dismutase (SOD) activity in hepatocytes were measured by spectrophotometry. The contents of reduced glutathione (GSH) and malondialdehyde (MDA) were found to be in the range of 75 ~ 600 渭 mol/L for 4 h. Results hydrogen peroxide could inhibit the proliferation of two kinds of hepatocytes in a dose-and time-dependent manner and promote the release of alt and LDH into the culture medium. The activity of SOD and GSH in the cells decreased significantly, which indicated that the two models of oxidative injury of hepatocytes were successfully constructed, and the best condition for oxidative stress injury of Hep G2 and Chang liver cells was to select 300 渭 mol/L H 2O 2 for 4 h. The results showed that the growth inhibition rates of Hep G2 and Chang liver cells were 62% and 76, respectively. The activities of Hep G 2 and Chang liver were 18.2 鹵0.2nmol / mg prot. Conclusion Hep G2 and Chang liver cells could be used to prepare the model of oxidative stress cell injury. The results showed that the MDA content of Hep G2 and Chang liver cells were 62% and 76, respectively. Conclusion both Hep G2 and Chang liver cells can be used to prepare the model of oxidative stress cell injury. The results showed that the MDA content of Hep G 2 and Chang liver cells were 62% and 76, respectively. Conclusion both Hep G2 and Chang liver cells can be used to prepare the model of oxidative stress cell injury. The results showed that the MDA content of Hep G 2 and Chang liver cells were 7. 8 鹵0. 9 and 8. 6 鹵1. 1 nmol / mg prot, respectively.
【作者單位】： 延邊大學(xué)醫(yī)學(xué)院生物化學(xué)與分子生物學(xué)教研室;
【基金】：國家自然科學(xué)基金(81160539;81360651)
【分類號(hào)】：R575

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