本文關鍵詞： BAFF Th細胞 幽門螺桿菌 十二指腸潰瘍　出處：《實用醫(yī)學雜志》2015年20期 　論文類型：期刊論文

【摘要】：目的:分析幽門螺桿菌誘發(fā)十二指腸潰瘍患者血清中B淋巴細胞活化因子(BAFF)表達情況,探討B(tài)AFF對Th17細胞分化的影響。方法:ELISA法測患者血清中BAFF與IL-17濃度;MTT法測T細胞與幽門螺桿菌共培養(yǎng)后存活率;流式細胞術測BAFF-R表達;RT-PCR、Western blot、ELISA法測RORγt和RORα及IL-17、IL-21、IL-22的m RNA和蛋白表達。結(jié)果:與對照相比,幽門螺桿菌陽性患者血清中BAFF與IL-17表達水平顯著增高(P0.05),兩者表達水平呈正相關(r=0.87,P0.01)。BAFF處理后,幽門螺桿菌誘導的T細胞生存活力較對照增強,BAFF-R表達增加,Th17細胞標志性轉(zhuǎn)錄因子RORγt和RORα及細胞因子IL-17、IL-21、IL-22的m RNA和蛋白表達水平均明顯高于對照(P0.05)。結(jié)論:幽門螺桿菌能引起十二指腸潰瘍患者體內(nèi)BAFF高表達,進而促進Th17細胞分化。
[Abstract]:Objective: to analyze the expression of B lymphocyte activating factor (BAFF) in serum of patients with duodenal ulcer induced by Helicobacter pylori. To investigate the effect of BAFF on the differentiation of Th17 cells. Methods the survival rate of T cells and Helicobacter pylori co-cultured was measured by BAFF and IL-17 concentration in serum of patients. Flow cytometry was used to detect the expression of ROR 緯 t and ROR 偽, and the m RNA and protein of ROR 偽 and IL-17 IL-21, IL-22. Results: compared with the control group, the expression of BAFF and IL-17 in the serum of patients with Helicobacter pylori positive was significantly higher than that in the control group. The expression of BAFF and IL-17 in serum of patients with Helicobacter pylori positive was significantly higher than that of the control group. The survival activity of T cells induced by Helicobacter pylori was significantly higher than that of the control group. The expression levels of ROR 緯 t and ROR 偽, as well as the expression levels of m RNA and protein of IL-17, IL-21, IL-22 in Th17 cells were significantly higher than those in the control group P0.050.Conclusion: Helicobacter pylori is more potent than the control group. Can cause high expression of BAFF in patients with duodenal ulcer. And then promote the differentiation of Th17 cells.
【作者單位】： 陜西省康復醫(yī)院消化內(nèi)科;
【分類號】：R573.1

【參考文獻】

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