本文關(guān)鍵詞： 乙醇 枯否細(xì)胞(KCs) 吞噬功能 微絲 膜流動性　出處：《中國公共衛(wèi)生》2017年12期 　論文類型：期刊論文

【摘要】：目的觀察乙醇對體外培養(yǎng)枯否細(xì)胞(KCs)吞噬功能、微絲表達(dá)和膜流動性影響。方法經(jīng)門靜脈插管膠原酶灌流消化肝臟,密度梯度離心分離KCs;體外培養(yǎng)KCs中加入不同劑量乙醇(1、2、4 g/L)作用3、24 h,采用聚苯乙烯乳珠吞噬實(shí)驗(yàn)、熒光染色法和熒光光度法檢測KCs的吞噬功能、微絲表達(dá)、肌動蛋白含量及膜流動性。結(jié)果 1、2、4 g/L乙醇組作用3、24 h KCs吞噬聚苯乙烯乳珠數(shù)量分別為(16.05±3.69)、(15.86±4.50)、(13.86±4.69)和(13.95±4.51)、(14.35±6.52)、(9.71±3.34)個(gè),明顯低于對照組[分別為(20.13±5.26)、(20.30±8.57)個(gè)](P0.05);4 g/L乙醇組(作用24 h)KCs吞噬功能明顯低于1、2 g/L乙醇組(P0.05);各劑量乙醇組(作用3 h)KCs微絲排列紊亂,但肌動蛋白含量無明顯變化;與對照組(5.85±1.13)比較,2、4 g/L乙醇組KCs膜流動性[(2.30±1.08)、(1.62±0.30)]明顯降低(P0.05),呈劑量依賴性。結(jié)論乙醇可降低體外KCs的吞噬功能,其機(jī)制可能與乙醇致KCs膜流動性下降和微絲排列紊亂有關(guān)。
[Abstract]:Objective to observe the effects of ethanol on the phagocytosis, microfilament expression and membrane fluidity of Kupffer cells in vitro. KCswere isolated by density gradient centrifugation and treated with different dosages of ethanolol (1g / L, 2g / L) for 3h for 24 h. The phagocytosis of KCs was detected by fluorescence staining and fluorescence spectrophotometry, and the expression of microfilaments was detected by fluorescence staining and fluorescence spectrophotometry. Results the content of actin and membrane fluidity in the ethanol group were 16.05 鹵3.69 KCs and 13.86 鹵4.69, respectively, and 14.35 鹵6.529.35 鹵6.529.71 鹵3.34 in the ethanol group (n = 1, n = 2, n = 2, n = 2, n = 4) and in the ethanol group (n = 3, 24 h), respectively, and the results showed that the content of actin and membrane fluidity were 15.86 鹵4.50 and 14.35 鹵6.529.71 鹵3.34, respectively. The phagocytosis of KCs was significantly lower than that of the control group (20.13 鹵5.26) (20.30 鹵8.57), the phagocytosis of KCs was significantly lower in the ethanol group (24 h) than that in the control group (20. 13 鹵5. 26 鹵8. 57), but the content of actin was not significantly changed in each dose of ethanol group (3 h after exposure), and the phagocytosis of KCs was significantly lower than that in the 1 g / L ethanol group (P 0. 05). Compared with the control group (5.85 鹵1.13), the fluidity of KCs membrane in 2g / L ethanol group [2.30 鹵1.08 鹵0.30] significantly decreased in a dose-dependent manner. Conclusion ethanol can reduce the phagocytic function of KCs in vitro, and the mechanism may be related to the decrease of KCs membrane fluidity and the disorder of microfilament arrangement induced by ethanol.
【作者單位】： 山西醫(yī)科大學(xué)汾陽學(xué)院科技中心;山西醫(yī)科大學(xué)肝病研究所;
【基金】：山西醫(yī)科大學(xué)汾陽學(xué)院人才啟動基金(1302)
【分類號】：R575
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本文編號：1498863