本文關(guān)鍵詞： 嬰兒雙歧桿菌 小鼠 肝纖維化 刀豆蛋白　出處：《鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年01期 　論文類(lèi)型：期刊論文

【摘要】：目的:研究嬰兒雙歧桿菌對(duì)刀豆蛋白A(ConA)誘導(dǎo)的小鼠肝纖維化的作用及其機(jī)制。方法:36只小鼠隨機(jī)分為正常組、模型組和嬰兒雙歧桿菌組,每組12只。正常組靜脈注射生理鹽水,模型組和嬰兒雙歧桿菌組靜脈注射12.5 mg/kg ConA溶液,每周1次。每日通過(guò)灌胃給予正常組和模型組蒸餾水,嬰兒雙歧桿菌組通過(guò)灌胃給予0.5 g/kg嬰兒雙歧桿菌溶液,持續(xù)7周。檢測(cè)小鼠肝臟質(zhì)量指數(shù),小鼠血清肝功能生化指標(biāo)(ALT、AST、ALB),透明質(zhì)酸(HA),Ⅲ型前膠原肽(PCⅢ)的水平,以及小鼠肝組織Ⅰ型膠原(ColⅠ)、Ⅲ型膠原(ColⅢ)、基質(zhì)金屬蛋白酶抑制因子(TIMP)-1、基質(zhì)蛋白金屬酶(MMP)-1、MMP-2、MMP-9、腫瘤壞死因子α(TNF-α)、IL-6、IL-10的含量。結(jié)果:嬰兒雙歧桿菌能顯著降低ConA誘導(dǎo)小鼠的肝臟質(zhì)量指數(shù)(P0.05),改善血清肝功能生化指標(biāo)(P0.05),降低血清HA、PCⅢ水平(P0.05),降低肝組織中ColⅠ、ColⅢ、MMP-2、MMP-9、TIMP-1、TNF-α、IL-6含量(P0.05),升高小鼠肝組織中MMP-1、IL-10含量(P0.05)。結(jié)論:嬰兒雙歧桿菌具有明確的抗小鼠肝纖維化作用。
[Abstract]:Objective: to study the effect and mechanism of bifidobacterium infantis on liver fibrosis induced by concanavalin Cona in mice. Methods: thirty six mice were randomly divided into normal group, model group and bifidobacterium infantile group with 12 rats in each group. Model group and Bifidobacterium infantile group were intravenously injected with 12.5 mg/kg ConA solution once a week. The normal group and model group were given distilled water daily, and the infant bifidobacterium group was given 0.5 g / kg bifidobacterium infantile solution by gavage. After 7 weeks, the levels of liver mass index, serum biochemical indexes of liver function, ALTASTASA ALBX, hyaluronic acid Ham, type 鈪，

本文編號(hào)：1495972