本文關(guān)鍵詞：實(shí)時(shí)組織彈性成像評(píng)價(jià)大鼠免疫性肝炎肝纖維化程度的實(shí)驗(yàn)研究　出處：《山西醫(yī)科大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 超聲檢查 彈性成像 免疫性肝炎 纖維化 炎癥

【摘要】：目的:1.探討彈性參數(shù)評(píng)價(jià)大鼠免疫性肝炎肝纖維化分期的可行性。2.探討炎癥分級(jí)對(duì)彈性參數(shù)評(píng)價(jià)大鼠免疫性肝炎肝纖維化分期的影響。方法:60只SD雄性大鼠隨機(jī)分為正常對(duì)照組和實(shí)驗(yàn)組,正常對(duì)照組8只,實(shí)驗(yàn)組52只。實(shí)驗(yàn)組尾靜脈注射豬血清誘導(dǎo)免疫性肝炎,正常對(duì)照組尾靜脈注射生理鹽水。每隔兩周從實(shí)驗(yàn)組與對(duì)照組隨機(jī)選取大鼠進(jìn)行實(shí)時(shí)組織彈性成像(real-time tissue elastography,RTE)檢查,取得12個(gè)彈性參數(shù),包括應(yīng)變均值(MEAN)、標(biāo)準(zhǔn)偏差(SD)、藍(lán)色區(qū)域%(%AREA)、復(fù)雜度(COMP)、峰度(KURT)、偏度(SKEW)、對(duì)比度(CONT)、均等性(ENT)、雜亂度(IDM)、一致性(ASM)、相關(guān)性(CORR)及肝纖維化指數(shù)(LF Index)。隨后處死大鼠,取肝組織進(jìn)行Masson及HE染色,并根據(jù)肝纖維化分期(Masson染色)及炎癥分級(jí)(HE染色)將實(shí)驗(yàn)組大鼠分別分為S0~S4組和G0~G4組。組間比較采用Kruskal-Wallis檢驗(yàn),相關(guān)分析采用Spearman秩相關(guān)分析,構(gòu)建受試者工作(receiver operating characteristic,ROC)曲線,取得截?cái)嘀挡⑶以u(píng)價(jià)LF Index診斷效能。結(jié)果:除了COMP、KURT及CORR,其余9個(gè)彈性參數(shù)組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),其中肝纖維化分期和LF Index相關(guān)性最高(r=0.809,P0.05)。除了KURT和CORR,其余10個(gè)彈性參數(shù)組間比較差異統(tǒng)計(jì)學(xué)意義(P0.05),其中肝炎癥分級(jí)和LF Index相關(guān)性最高(r=0.766,P0.05)。以LF Index繪制肝纖維化分期ROC曲線,S≥S1曲線下面積為0.940,截?cái)嘀禐?.35,敏感度為97.1%,特異度為73.3%;S≥S2曲線下面積為0.865,截?cái)嘀禐?.99,敏感度為78.6%,特異度為81.0%;S≥S3曲線下面積為0.924,截?cái)嘀禐?.48,敏感度為94.4%,特異度為87.1%;S=S4曲線下面積為0.933,截?cái)嘀禐?.05,敏感度為87.5%,特異度為95.1%。LF Index可以有效地評(píng)價(jià)肝纖維化分期,其準(zhǔn)確性為79.6%(39/49)。但是,有20.4%(10/49)的結(jié)果為假陽(yáng)性。結(jié)論:彈性參數(shù)可以有效地評(píng)價(jià)大鼠免疫性肝炎肝纖維化分期。但是,炎癥壞死活動(dòng)作為重要的粘性因素會(huì)影響其準(zhǔn)確性。
[Abstract]:Objective : To evaluate the feasibility of evaluating the stage of liver fibrosis in rats with immune hepatitis by elastic parameters . Methods : 60 SD male rats were randomly divided into normal control group and experimental group . Results : In addition to COMP , KURT and CORR , there was a significant difference among the remaining nine groups ( P0.05 ) , among which the correlation between the stage of liver fibrosis and LF index was the highest ( r = 0.809 , P0.05 ) . In addition to the KURT and CORR , the difference was statistically significant among the remaining 10 elastic parameters ( P0.05 ) , among which the correlation between liver inflammation grade and LF index was the highest ( r = 0.766 , P0.05 ) . The ROC curve of hepatic fibrosis was drawn with LF Index , the area of S 鈮，

本文編號(hào)：1382709