本文關(guān)鍵詞：聚合酶鏈反應(yīng)法快速診斷肝硬化患者腹水細(xì)菌感染臨床應(yīng)用研究　出處：《中華醫(yī)院感染學(xué)雜志》2016年19期 　論文類型：期刊論文

  更多相關(guān)文章： 聚合酶鏈反應(yīng) 肝硬化 自發(fā)性腹膜炎 SrRNA

【摘要】：目的應(yīng)用聚合酶鏈反應(yīng)(PCR)檢測(cè)細(xì)菌16SrRNA基因,探討其在輔助診斷肝硬化自發(fā)性腹膜炎(SBP)中的臨床應(yīng)用價(jià)值。方法選取2014年12月-2015年10月醫(yī)院感染病科77例肝硬化失代償期合并腹水患者進(jìn)行研究,以細(xì)菌16SrRNA基因?yàn)榘行蛄?在保守區(qū)和特異區(qū)分別選取特異性序列合成引物,建立快速診斷方法,檢測(cè)臨床肝硬化失代償期患者腹水中細(xì)菌DNA,并將其結(jié)果與傳統(tǒng)方法進(jìn)行比較,數(shù)據(jù)采用SPSS16.0統(tǒng)計(jì)軟件進(jìn)行處理。結(jié)果 77份臨床樣本中PCR法16SrRNA通用引物陽(yáng)性擴(kuò)增50例,陽(yáng)性率64.9%,特異引物陽(yáng)性擴(kuò)增15例,陽(yáng)性率19.5%;以腹水細(xì)菌培養(yǎng)陽(yáng)性或(和)腹水PMN≥250×106/L或(和)腹膜刺激征陽(yáng)性且正規(guī)抗感染治療有效者作為診斷SBP金標(biāo)準(zhǔn),此時(shí)PCR法靈敏度為100.0%,特異度為54.0%,陽(yáng)性預(yù)測(cè)值為54.0%,陰性預(yù)測(cè)值為100.0%,二者差異有統(tǒng)計(jì)學(xué)意義(χ~2=22.453,P0.01)。結(jié)論與傳統(tǒng)方法相比,PCR法是一種更敏感且簡(jiǎn)便快速的方法,在肝硬化失代償期患者腹水細(xì)菌檢測(cè)中具有一定的臨床應(yīng)用價(jià)值。
[Abstract]:Objective to detect 16s rRNA gene by polymerase chain reaction (PCR). Study on the role of SBP in the Auxiliary diagnosis of spontaneous peritonitis in cirrhotic patients. Methods from December 2014 to October 2015, 77 patients with ascites in decompensated stage of liver cirrhosis were studied. Using 16s rRNA as the target sequence, primers were synthesized in the conserved region and the specific region, and a rapid diagnostic method was established to detect bacterial DNA in ascites of patients with decompensated cirrhosis. The results were compared with the traditional method and the data were processed by SPSS16.0 software. Results 50 cases of PCR 16s rRNA universal primer positive amplification were performed in 77 clinical samples. The positive rate was 64.9% and the positive rate of specific primers was 19.5% in 15 cases. Patients with positive bacterial culture or / and ascites PMN 鈮，

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