本文關(guān)鍵詞：聚合酶鏈反應(yīng)法快速診斷肝硬化患者腹水細菌感染臨床應(yīng)用研究　出處：《中華醫(yī)院感染學(xué)雜志》2016年19期 　論文類型：期刊論文

  更多相關(guān)文章： 聚合酶鏈反應(yīng) 肝硬化 自發(fā)性腹膜炎 SrRNA

【摘要】：目的應(yīng)用聚合酶鏈反應(yīng)(PCR)檢測細菌16SrRNA基因,探討其在輔助診斷肝硬化自發(fā)性腹膜炎(SBP)中的臨床應(yīng)用價值。方法選取2014年12月-2015年10月醫(yī)院感染病科77例肝硬化失代償期合并腹水患者進行研究,以細菌16SrRNA基因為靶序列,在保守區(qū)和特異區(qū)分別選取特異性序列合成引物,建立快速診斷方法,檢測臨床肝硬化失代償期患者腹水中細菌DNA,并將其結(jié)果與傳統(tǒng)方法進行比較,數(shù)據(jù)采用SPSS16.0統(tǒng)計軟件進行處理。結(jié)果 77份臨床樣本中PCR法16SrRNA通用引物陽性擴增50例,陽性率64.9%,特異引物陽性擴增15例,陽性率19.5%;以腹水細菌培養(yǎng)陽性或(和)腹水PMN≥250×106/L或(和)腹膜刺激征陽性且正規(guī)抗感染治療有效者作為診斷SBP金標準,此時PCR法靈敏度為100.0%,特異度為54.0%,陽性預(yù)測值為54.0%,陰性預(yù)測值為100.0%,二者差異有統(tǒng)計學(xué)意義(χ~2=22.453,P0.01)。結(jié)論與傳統(tǒng)方法相比,PCR法是一種更敏感且簡便快速的方法,在肝硬化失代償期患者腹水細菌檢測中具有一定的臨床應(yīng)用價值。
[Abstract]:Objective to detect 16s rRNA gene by polymerase chain reaction (PCR). Study on the role of SBP in the Auxiliary diagnosis of spontaneous peritonitis in cirrhotic patients. Methods from December 2014 to October 2015, 77 patients with ascites in decompensated stage of liver cirrhosis were studied. Using 16s rRNA as the target sequence, primers were synthesized in the conserved region and the specific region, and a rapid diagnostic method was established to detect bacterial DNA in ascites of patients with decompensated cirrhosis. The results were compared with the traditional method and the data were processed by SPSS16.0 software. Results 50 cases of PCR 16s rRNA universal primer positive amplification were performed in 77 clinical samples. The positive rate was 64.9% and the positive rate of specific primers was 19.5% in 15 cases. Patients with positive bacterial culture or / and ascites PMN 鈮，

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