【摘要】：目的:觀察在不同溫度條件下脊髓星形膠質(zhì)細(xì)胞劃痕損傷活化后的形態(tài)和活性改變,以探討亞低溫對脊髓損傷后反應(yīng)性星形膠質(zhì)細(xì)胞增生的影響。方法:體外原代培養(yǎng)新生SD大鼠脊髓星形膠質(zhì)細(xì)胞,以劃痕實(shí)驗(yàn)制備反應(yīng)性星形膠質(zhì)細(xì)胞。亞低溫選擇33℃,細(xì)胞培養(yǎng)48 h。實(shí)驗(yàn)分為對照組、劃痕組、亞低溫組和劃痕+亞低溫組。各組在相應(yīng)的時(shí)間點(diǎn)觀察細(xì)胞形態(tài),采用免疫熒光染色方法檢測Nestin陽性率,MTT比色法觀察細(xì)胞活性,PI染色方法觀察細(xì)胞凋亡程度。結(jié)果:與對照組和亞低溫組相比,劃痕組和劃痕+亞低溫組細(xì)胞胞體肥大,周圍突起增多、延展以及胞漿豐富,細(xì)胞生長率明顯升高。與劃痕組相比,劃痕+亞低溫組細(xì)胞變化減慢,周圍突起減少,細(xì)胞長入劃痕處所需時(shí)間增加,細(xì)胞Nestin陽性率、PI陽性率和細(xì)胞生長率明顯降低,各結(jié)果差異顯著(P0.01)。結(jié)論:劃痕損傷后星形膠質(zhì)細(xì)胞活化為反應(yīng)性星形膠質(zhì)細(xì)胞并會增生,亞低溫明顯抑制脊髓反應(yīng)性星形膠質(zhì)細(xì)胞的活化增生,并可以抑制星形膠質(zhì)細(xì)胞的凋亡。
[Abstract]:Aim: to observe the morphological and active changes of spinal cord astrocytes after scratch injury at different temperatures, and to explore the effect of mild hypothermia on reactive astrocytes proliferation after spinal cord injury. Methods: the spinal cord astrocytes of neonatal SD rats were cultured in vitro and reactive astrocytes were prepared by scratch test. The cells were cultured at 33 鈩，

本文編號：2504304