【摘要】：目的:觀察異氟烷預(yù)處理是否通過增強大鼠海馬內(nèi)M2型小膠質(zhì)細胞活化,減輕炎癥反應(yīng)改善電磁脈沖所致神經(jīng)元損傷。方法:24只SD大鼠隨機分為4組,分別為對照組(CON組)、異氟烷預(yù)處理組(IP組)和電磁脈沖照射組(EMP組)和異氟烷預(yù)處理加電磁脈沖照射組(IP+EMP組)。各組于照射后24 h進行尼氏染色;采用Western Blot和免疫熒光組織化學(xué)染色方法檢測M2型小膠質(zhì)細胞標(biāo)志物Arg-1的表達;并采用ELISA方法測定海馬組織中TNF-α和IL-1β的含量。結(jié)果:與CON組相比,EMP組海馬齒狀回顆粒細胞的尼氏體明顯減少,而與EMP組相比,IP+EMP組海馬齒狀回顆粒細胞的尼氏體明顯增多增大。Western Blot結(jié)果顯示:與CON組相比,EMP組海馬內(nèi)Arg-1蛋白的表達明顯減少(P0.05),與EMP組相比,IP+EMP組Arg-1蛋白的表達明顯增加(P0.05)。免疫熒光染色結(jié)果顯示:與CON組相比,EMP組海馬內(nèi)Arg-1的表達減弱;而與EMP組相比,IP+EMP組Arg-1的表達明顯增強。EMP組海馬組織中炎癥因子TNF-α和IL-1β的含量較CON組增加(P0.05),IP+EMP組腦組織中炎癥因子TNF-α和IL-1β的含量較EMP組減少(P0.05)。結(jié)論:異氟烷預(yù)處理可通過增加M2型小膠質(zhì)細胞,減少腦組織中炎癥因子含量,減輕EMP所致神經(jīng)元損傷。
[Abstract]:Aim: to observe whether isohalothane pretreatment can improve neuronal injury induced by electromagnetic pulse by enhancing the activation of M2 microglia in rat hippocampus and reducing inflammation. Methods: 24 SD rats were randomly divided into 4 groups: control group (CON group), isohalothane pretreatment group (IP group), electromagnetic pulse irradiation group (EMP group) and isohalothane pretreatment plus electromagnetic pulse irradiation group (IP EMP group). Nissl staining was performed 24 hours after irradiation, the expression of M2 microglia marker Arg-1 was detected by Western Blot and immunofluorescence histochemical staining, and the contents of TNF- 偽 and IL-1 尾 in hippocampus were measured by ELISA. Results: compared with CON group, the nainite of dentate gyrus granulosa cells in EMP group was significantly lower than that in EMP group, while that in, IP EMP group was significantly higher than that in EMP group. Western Blot results showed that the expression of Arg-1 protein in hippocampus of EMP group was significantly lower than that of CON group (P 0.05), and the expression of Arg-1 protein in, IP EMP group was significantly higher than that of EMP group (P 0.05). The results of immunofluorescence staining showed that the expression of Arg-1 in hippocampus of EMP group was lower than that of CON group, while the expression of Arg-1 in, IP EMP group was significantly higher than that of EMP group. The contents of TNF- 偽 and IL-1 尾 in hippocampal tissue of EMP group were higher than those of CON group (P 0.05). The contents of TNF- 偽 and IL-1 尾 in brain tissue of), IP EMP group were lower than those of EMP group (P 0.05). Conclusion: isofluoroalkane pretreatment can reduce the content of inflammatory factors in brain tissue and reduce neuronal injury induced by EMP by increasing M2 microglia.
【作者單位】： 西安市第四醫(yī)院麻醉科;第四軍醫(yī)大學(xué)唐都醫(yī)院麻醉科;第四軍醫(yī)大學(xué)放射醫(yī)學(xué)教研室;
【基金】：國家自然科學(xué)基金(3157040065)
【分類號】：R614

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