【摘要】：目的探討內(nèi)質(zhì)網(wǎng)跨膜蛋白肌醇需求酶(IRE)1α介導(dǎo)的凋亡通路在骨關(guān)節(jié)炎(OA)發(fā)病中的作用及機(jī)制。方法新西蘭兔膝關(guān)節(jié)注射木瓜蛋白酶模擬OA模型,HE染色檢測(cè)OA關(guān)節(jié)軟骨以及關(guān)節(jié)滑膜的形態(tài),ELISA法檢測(cè)OA關(guān)節(jié)滑膜中炎性因子白細(xì)胞介素(IL)-6的含量。檢測(cè)閉合性松解術(shù)關(guān)節(jié)軟骨的組織形態(tài)以及IL-6的含量。Western印跡檢測(cè)IRE1α介導(dǎo)的凋亡通路在OA滑膜中的表達(dá)水平。結(jié)果 HE染色結(jié)果顯示木瓜蛋白酶誘導(dǎo)的OA模型中,關(guān)節(jié)軟骨細(xì)胞發(fā)生明顯壞死,纖維組織變性、機(jī)化以及纖維蛋白壞死物沉積,ELISA結(jié)果顯示關(guān)節(jié)滑膜中的IL-6水平明顯升高,閉合性松解術(shù)后的OA關(guān)節(jié)軟骨細(xì)胞凋亡減少,且關(guān)節(jié)滑膜中IL-6水平也明顯降低。蛋白水平顯示OA膝關(guān)節(jié)滑膜中IRE1α的磷酸化水平以及Caspase3的表達(dá)水平明顯升高,介導(dǎo)關(guān)節(jié)軟骨細(xì)胞的凋亡,而閉合性松解術(shù)后Caspase3的表達(dá)水平降低。結(jié)論閉合性松解術(shù)可緩解OA的癥狀,抑制IRE1α-Caspase3信號(hào)通路介導(dǎo)的凋亡作用。
[Abstract]:Objective to investigate the role and mechanism of apoptosis pathway mediated by endoplasmic reticulum transmembrane protein inositol demand enzyme (IRE) 1 偽 in the pathogenesis of osteoarthritis (OA). Methods New Zealand rabbits were injected with papain to simulate OA model. The morphology of cartilage and synovium of OA joint was detected by HE staining, and the content of inflammatory factor (IL)-6 in synovium of OA joint was detected by ELISA method. The tissue morphology and IL-6 content of articular cartilage after closed loosening were detected, and the expression of IRE1 偽 mediated apoptosis pathway in OA synovium was detected by Western blot. Results the results of HE staining showed that in the OA model induced by papain, the articular cartilage cells were obviously necrotic, fibrous tissue denaturation, mechanization and fibrin necrosis were deposited. ELISA results showed that the level of IL-6 in synovium of joint was significantly increased. After closed loosening, the apoptosis of cartilage cells in OA joint decreased, and the level of IL-6 in synovium also decreased significantly. The protein level showed that the phosphorylation level of IRE1 偽 and the expression of Caspase3 in synovial membrane of OA knee joint were significantly increased, which mediated the apoptosis of articular cartilage cells, while the expression level of Caspase3 decreased after closed loosening. Conclusion closed loosening can relieve the symptoms of OA and inhibit the apoptosis mediated by IRE1 偽-Caspase3 signaling pathway.
【作者單位】： 黑龍江中醫(yī)藥大學(xué)附屬第二醫(yī)院骨科;
【基金】：黑龍江中醫(yī)藥大學(xué)科研基金項(xiàng)目(201310)
【分類號(hào)】：R684.3

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