【摘要】：研究目的:通過比較波形蛋白,β-連環(huán)蛋白,α-平滑肌動(dòng)蛋白3個(gè)因子在泡型包蟲病患者正常肝組織以及邊緣帶組織中的表達(dá)差異來明確泡型包蟲病疾病進(jìn)展中是否存在EMT現(xiàn)象。研究方法:將29例經(jīng)手術(shù)治療獲得的肝臟組織分為兩類:正常肝組織與邊緣帶組織(由病理科定位)。制作組織芯片并染色。在顯微鏡下判讀SMA、β-catenin、VIM的免疫組織化學(xué)表達(dá)。計(jì)算邊緣帶與正常肝組織的平均表達(dá)率,并應(yīng)用統(tǒng)計(jì)學(xué)得出此次實(shí)驗(yàn)的結(jié)論。結(jié)果:1.在邊緣帶及正常組織中波形蛋白,β-連環(huán)蛋白,α-平滑肌動(dòng)的陽(yáng)性細(xì)胞表達(dá)率分別為52.79%±6.92%、12.97%±4.43%/48.56%±16.80%、16.14%±6.62%/62.72%±16.96%、11.50%±7.05%。組間對(duì)比均有顯著性差異。2.存在侵犯或轉(zhuǎn)移的泡型包蟲病灶邊緣帶及無侵犯或轉(zhuǎn)移的邊緣帶中波形蛋白,β-連環(huán)蛋白,α-平滑肌動(dòng)的陽(yáng)性細(xì)胞表達(dá)率分別為53.04%±8.05%、52.33%±4.67%/49.98%±17.64%、46.03%±15.87%/66.21%±16.99%、56.51%±15.96%。表達(dá)在組間均無顯著性差異。3.在無侵犯或轉(zhuǎn)移的泡型包蟲病灶邊緣帶及正常肝組織中波形蛋白,β-連環(huán)蛋白,α-平滑肌動(dòng)的陽(yáng)性細(xì)胞表達(dá)率分別為56.51%±15.95%、11.50%±7.05%/46.03%±15.87%、16.14%±6.62%/56.51%±15.95%、11.50%±7.05%。組間對(duì)比均有顯著性差異。結(jié)論:1.與正常肝組織相比,邊緣帶中的波形蛋白,β-連環(huán)蛋白,α-平滑肌動(dòng)蛋白的表達(dá)均明顯上升,表明了肝泡型包蟲病發(fā)生、發(fā)展中,存在上皮-間質(zhì)轉(zhuǎn)化。2.在有侵犯轉(zhuǎn)移和無侵犯轉(zhuǎn)移的邊緣帶組織中,3個(gè)因子無明顯差異,表明即使在尚未表現(xiàn)出侵襲性及轉(zhuǎn)移能力的病灶中,仍然存在EMT現(xiàn)象,病灶仍具有侵襲的能力與潛力。
[Abstract]:Objective: to compare vimentin, 尾-catenin, The expression difference of 偽-smooth actin (偽-actin) in normal liver tissues and marginal zone tissues of patients with alveolar hydatid disease was studied to determine whether there was EMT phenomenon in the progression of alveolar hydatid disease. Methods: twenty-nine cases of liver tissue obtained by surgical treatment were divided into two groups: normal liver tissue and marginal zone tissue (located by Pathology Department). Tissue chips were made and stained. The immunohistochemical expression of SMA, 尾-catenin,VIM was identified under microscope. The average expression rate of marginal zone and normal liver tissue was calculated, and the conclusion of this experiment was obtained by using statistics. Results: 1. The positive rates of vimentin, 尾-catenin and 偽-smooth muscle in marginal zone and normal tissue were 52.79% 鹵6.92%, 12.97% 鹵4.43%, 48.56% 鹵16.80%, respectively. 16.14% 鹵6.62%, 62.72% 鹵16.96%, 11.50% 鹵7.05%, respectively. There were significant differences between the two groups. 2. The positive rates of vimentin, 尾-catenin and 偽-smooth muscle were 53.04% 鹵8.05% in marginal zone and no invasion or metastasis in marginal zone of hydatid foci with invasion or metastasis, respectively. 52.33% 鹵4.67%, 49.98% 鹵17.64%, 46.03% 鹵15.87%, 66.21% 鹵16.99%, 56.51% 鹵15.96%, respectively. There was no significant difference in expression among groups. 3. The positive rates of vimentin, 尾-catenin and 偽-smooth muscle were 56.51% 鹵15.95% in the marginal zone and normal liver tissue of non-invasive or metastatic hydatid lesions, respectively. 11.50% 鹵7.05%, 46.03% 鹵15.87%, 16.14% 鹵6.62%, 56.51% 鹵15.95%, 11.50% 鹵7.05%, respectively. There were significant differences between groups. Conclusions: 1. Compared with normal liver tissues, the expression of vimentin, 尾-catenin and 偽-smooth actin in marginal zone increased significantly, indicating the occurrence and development of hepatic alveolar echinococcosis. 2. There was no significant difference among the three factors in the marginal zone tissues with and without invasion metastasis, which indicated that there was still EMT phenomenon in the lesions without invasion and metastasis, and that the lesions still had the ability and potential of invasiveness. The results showed that there was no significant difference in the three factors between the invasive and non-invasive marginal zone tissues.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R657.3

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