【摘要】：目的:探討焦脫鎂葉綠酸-a甲酯介導(dǎo)的光動(dòng)力療法(methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT)對(duì)人骨肉瘤MG-63細(xì)胞遷移及侵襲的影響及其可能機(jī)制。方法:采用CCK-8檢測(cè)經(jīng)MPPa-PDT處理后不同時(shí)間點(diǎn)的人骨肉瘤MG-63細(xì)胞增殖活性;劃痕實(shí)驗(yàn)及Transwell實(shí)驗(yàn)檢測(cè)細(xì)胞遷移、侵襲能力;Western blotting檢測(cè)E-鈣黏蛋白(E-cad)和基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)-2、-9蛋白的表達(dá)水平。結(jié)果:MPPa-PDT處理MG-63細(xì)胞12、24及48 h后MPPa-PDT組細(xì)胞增殖能力明顯較對(duì)照組、MPPa組和LED組細(xì)胞降低(均P0.05),且MPPa-PDT組細(xì)胞的劃痕愈合能力、遷移活力及侵襲能力也較這3組細(xì)胞顯著下降(均P0.05);MPPa-PDT組細(xì)胞E-cad的表達(dá)顯著高于其他3組,MMP-2、MMP-9的表達(dá)則明顯低于其他3組(均P0.05)。結(jié)論:MPPa-PDT抑制人骨肉瘤MG-63細(xì)胞的侵襲和遷移能力;上調(diào)E-cad的表達(dá),下調(diào)MMP-2、-9的表達(dá)可能是其作用機(jī)制之一。
[Abstract]:Aim: to investigate the effect of photodynamic therapy (methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT) mediated by pyromagnesium chlorophyllin-a methyl ester on migration and invasion of human osteosarcoma MG-63 cells and its possible mechanism. Methods: CCK-8 was used to detect the proliferation activity of human osteosarcoma MG-63 cells treated with MPPa-PDT at different time points, scratch test and Transwell test were used to detect the migration and invasion ability of the cells. The expression levels of E-cadherin (E-cad), matrix metalloproteinases (matrix metalloproteinase,MMP)-2, and protein 9 were detected by Western blotting. Results: the proliferation ability of MG-63 cells treated with MPPa-PDT for 12 h, 24 h and 48 h was significantly lower than that of control group, MPPa group and LED group (P 0.05), and the ability of scratch healing in MPPa-PDT group was significantly lower than that of control group, MPPa group and LED group (P 0.05). The migration activity and invasion ability of the three groups were significantly lower than those of the three groups (P 0.05). The expression of E-cad in MPPa-PDT group was significantly higher than that in the other 3 groups, while the expression of MMP-2,MMP-9 in the other 3 groups was significantly lower than that in the other 3 groups (P 0.05). Conclusion: MPPa-PDT can inhibit the invasion and migration of human osteosarcoma MG-63 cells, up-regulate the expression of E-cad and down-regulate the expression of MMP-2,-9 may be one of its mechanisms.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院骨科
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81572634) 重慶市教育委員會(huì)研究生科研創(chuàng)新項(xiàng)目(No.CYS15141)~~
【分類號(hào)】：R738.1

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