【摘要】：目的:探討焦脫鎂葉綠酸-a甲酯介導的光動力療法(methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT)對人骨肉瘤MG-63細胞遷移及侵襲的影響及其可能機制。方法:采用CCK-8檢測經(jīng)MPPa-PDT處理后不同時間點的人骨肉瘤MG-63細胞增殖活性;劃痕實驗及Transwell實驗檢測細胞遷移、侵襲能力;Western blotting檢測E-鈣黏蛋白(E-cad)和基質(zhì)金屬蛋白酶(matrix metalloproteinase,MMP)-2、-9蛋白的表達水平。結(jié)果:MPPa-PDT處理MG-63細胞12、24及48 h后MPPa-PDT組細胞增殖能力明顯較對照組、MPPa組和LED組細胞降低(均P0.05),且MPPa-PDT組細胞的劃痕愈合能力、遷移活力及侵襲能力也較這3組細胞顯著下降(均P0.05);MPPa-PDT組細胞E-cad的表達顯著高于其他3組,MMP-2、MMP-9的表達則明顯低于其他3組(均P0.05)。結(jié)論:MPPa-PDT抑制人骨肉瘤MG-63細胞的侵襲和遷移能力;上調(diào)E-cad的表達,下調(diào)MMP-2、-9的表達可能是其作用機制之一。
[Abstract]:Aim: to investigate the effect of photodynamic therapy (methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT) mediated by pyromagnesium chlorophyllin-a methyl ester on migration and invasion of human osteosarcoma MG-63 cells and its possible mechanism. Methods: CCK-8 was used to detect the proliferation activity of human osteosarcoma MG-63 cells treated with MPPa-PDT at different time points, scratch test and Transwell test were used to detect the migration and invasion ability of the cells. The expression levels of E-cadherin (E-cad), matrix metalloproteinases (matrix metalloproteinase,MMP)-2, and protein 9 were detected by Western blotting. Results: the proliferation ability of MG-63 cells treated with MPPa-PDT for 12 h, 24 h and 48 h was significantly lower than that of control group, MPPa group and LED group (P 0.05), and the ability of scratch healing in MPPa-PDT group was significantly lower than that of control group, MPPa group and LED group (P 0.05). The migration activity and invasion ability of the three groups were significantly lower than those of the three groups (P 0.05). The expression of E-cad in MPPa-PDT group was significantly higher than that in the other 3 groups, while the expression of MMP-2,MMP-9 in the other 3 groups was significantly lower than that in the other 3 groups (P 0.05). Conclusion: MPPa-PDT can inhibit the invasion and migration of human osteosarcoma MG-63 cells, up-regulate the expression of E-cad and down-regulate the expression of MMP-2,-9 may be one of its mechanisms.
【作者單位】： 重慶醫(yī)科大學附屬第一醫(yī)院骨科
【基金】：國家自然科學基金項目(No.81572634) 重慶市教育委員會研究生科研創(chuàng)新項目(No.CYS15141)~~
【分類號】：R738.1

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