【摘要】：背景:理論上組織工程竇房結(jié)移植入體內(nèi)可以建立新的起搏點,并且可以隨著機體發(fā)育,達到治療竇房結(jié)綜合征的目的,但目前仍處于最初級的階段,需要進行不斷的探索。目的:總結(jié)綜述現(xiàn)階段組織工程竇房結(jié)構(gòu)建的不同方法及心臟組織工程血管化的研究進展。方法:由第一作者檢索Pub Med數(shù)據(jù)庫及CNKI全文數(shù)據(jù)庫1984年至2016年的相關(guān)文獻,并進行篩選,歸納和總結(jié)。英文檢索詞為"Vascularize,Tissue Engineering,Sinus Node",中文檢索詞為"血管化、組織工程、竇房結(jié)"。闡述組織工程竇房結(jié)的構(gòu)建方法,總結(jié)歸納了各種心臟組織工程血管化的重要性及策略,分析組織工程竇房結(jié)血管化應(yīng)用的方法,最后組織工程竇房結(jié)血管化進行總結(jié)和展望。結(jié)果與結(jié)論:起搏種子細胞的選擇大致為竇房結(jié)原代細胞,脂肪、骨髓間充質(zhì)干細胞,胚胎干細胞等。組織工程支架材料更是種類繁多,活體的竇房結(jié)本身就處于膠原支架上,因此膠原類材料更為適用于組織工程竇房結(jié)。3D打印技術(shù)、細胞膜片技術(shù)使血管化組織工程竇房結(jié)的構(gòu)建和移植成為可能。移植早期的血液供應(yīng),是竇房結(jié)移植是否成功的關(guān)鍵,組織工程竇房結(jié)血管化的相關(guān)文獻在仍處于空白狀態(tài),內(nèi)皮祖細胞作為成血管能力較強的細胞已經(jīng)有了巨大的進展,但是仍有一些缺陷,還面臨著重大的挑戰(zhàn)。
[Abstract]:Background: in theory, tissue engineering sinus node transplantation into the body can establish a new pacing point, and with the development of the body, can achieve the purpose of treating sinus node syndrome, but at present, it is still in the most primary stage, and need to be continuously explored. Objective: to summarize the different methods of tissue engineering sinus node construction and the research progress of heart tissue engineering vascularization. Methods: Pub Med database and CNKI full text database were searched by the first author from 1984 to 2016. The key words were "Vascularize,Tissue Engineering,Sinus Node" in English and "vascularization, tissue engineering, sinus node" in Chinese. This paper expounds the construction methods of tissue engineering sinus node, summarizes the importance and strategy of various kinds of heart tissue engineering vascularization, analyzes the application methods of tissue engineering sinus node vascularization, and finally summarizes and prospects the vascularization of tissue engineering sinus node. Results and conclusion: the selection of pacemaker seed cells was mainly sinoatrial node primary cells, fat, bone marrow mesenchymal stem cells, embryonic stem cells and so on. Tissue engineering scaffolds have a wide variety of materials, and the living sinoatrial node itself is on the collagen scaffold, so collagen materials are more suitable for tissue engineering sinus node. 3D printing technology. Membrane patch technique makes it possible to construct and transplant the vascularized tissue engineered sinus node. The blood supply in the early stage of transplantation is the key to the success of sinoatrial node transplantation. The literature on vascularization of tissue engineering sinus node is still blank, and endothelial progenitor cells have made great progress as vascular forming cells. But there are still some flaws and major challenges.
【作者單位】： 濱州醫(yī)學(xué)院煙臺校區(qū);解放軍第二軍醫(yī)大學(xué);
【基金】：國家自然科學(xué)基金(81071603,30970737) 上海市自然科學(xué)重點基金(09JC1417700)~~
【分類號】：R318.08;R654

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