【摘要】：[目的]通過(guò)檢測(cè)不同軟骨退變程度的患者的關(guān)節(jié)組織轉(zhuǎn)換生物標(biāo)記物及血清Micro RNAs,評(píng)估其對(duì)骨性關(guān)節(jié)炎的診斷價(jià)值。[方法]選取2015年1月~2015年5月于本院行膝關(guān)節(jié)鏡手術(shù)或初次人工膝關(guān)節(jié)置換術(shù)的患者44例。術(shù)前留取血清、尿、關(guān)節(jié)液標(biāo)本。測(cè)定s-CTX-I、u-CTX-I、u-CTX-II、sf-CTX-II、s-COMP、u-COMP、sf-COMP、s-HA、sf-HA值,同時(shí)測(cè)定血清mi R-885-5p、mi R-let-7e、mi R-140-3p、mi R-28-3p及mi R-454的量。評(píng)估各生物標(biāo)記物的量與不同患者K-L分級(jí)、Outerbridge分級(jí)之間的相關(guān)性。并根據(jù)K-L分級(jí),分成r OA組(影像學(xué)OA改變組,radiological osteoarthritis,r OA)(K-L分級(jí)≥2級(jí))及非r OA組(K-L分級(jí)2級(jí)),比較兩組中生物標(biāo)記物含量的差異。同時(shí)記錄兩組患者的性別、年齡、身高、體重,分析各生物標(biāo)記物的影響因素。[結(jié)果]在測(cè)定的9項(xiàng)生物指標(biāo)中,僅s-CTX-I、s-COMP、s-HA的含量與K-L分級(jí)及Outerbridge評(píng)分呈正相關(guān),其余6項(xiàng)未見(jiàn)明顯相關(guān)性。r OA組s-CTX-I、s-COMP、s-HA的含量分別為(270.40±52.01)pg/ml、(227.10±71.82)ng/ml、(14.11±5.53)ng/ml,顯著高于非r OA組,后者分別為(230.22±38.15)pg/ml、(176.44±40.66)ng/ml、(9.06±2.76)ng/ml(P0.05),其余6項(xiàng)生物標(biāo)記物含量未見(jiàn)明顯差異。血清mi R-885-5p、mi R-let-7e、mi R-140-3p、mi R-28-3p及mi R-454的相對(duì)表達(dá)量隨著K-L分級(jí)逐漸增高,但與K-L分級(jí)及Outerbidge分級(jí)均無(wú)明顯相關(guān)性。[結(jié)論]血清CTX-I、COMP及HA的含量在OA診斷及病情評(píng)估中具有一定的意義,但易受其他代謝狀態(tài)、性別及年齡的影響。Micro RNAs能反映軟骨轉(zhuǎn)換狀態(tài),但對(duì)于OA的診斷價(jià)值仍需進(jìn)一步明確。
[Abstract]:[objective] to evaluate the diagnostic value of tissue change biomarker and serum Micro RNAs, in patients with osteoarthritis. [methods] 44 cases of knee arthroscopy or initial arthroplasty were performed in our hospital from January 2015 to May 2015. Samples of serum, urine and joint fluid were collected before operation. The values of s-CTX-Inu-CTX-IIOS-COMPX-IIS-COMPX-COMPS-HAMPX-HA, and serum mi R-885-5pnmi R-let-7eguanmi R-140-3p, were determined. Mi R-28-3p and mi R-454. To evaluate the correlation between the amount of biomarkers and K-L grade and Outerbridge grade in different patients. According to K-L grade, the patients were divided into r OA group (imaging OA change group, radiological osteoarthritis,r OA) (K-L grade 鈮，

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