發(fā)布時間：2018-12-29 14:38

【摘要】：[目的]通過檢測不同軟骨退變程度的患者的關節(jié)組織轉換生物標記物及血清Micro RNAs,評估其對骨性關節(jié)炎的診斷價值。[方法]選取2015年1月~2015年5月于本院行膝關節(jié)鏡手術或初次人工膝關節(jié)置換術的患者44例。術前留取血清、尿、關節(jié)液標本。測定s-CTX-I、u-CTX-I、u-CTX-II、sf-CTX-II、s-COMP、u-COMP、sf-COMP、s-HA、sf-HA值,同時測定血清mi R-885-5p、mi R-let-7e、mi R-140-3p、mi R-28-3p及mi R-454的量。評估各生物標記物的量與不同患者K-L分級、Outerbridge分級之間的相關性。并根據(jù)K-L分級,分成r OA組(影像學OA改變組,radiological osteoarthritis,r OA)(K-L分級≥2級)及非r OA組(K-L分級2級),比較兩組中生物標記物含量的差異。同時記錄兩組患者的性別、年齡、身高、體重,分析各生物標記物的影響因素。[結果]在測定的9項生物指標中,僅s-CTX-I、s-COMP、s-HA的含量與K-L分級及Outerbridge評分呈正相關,其余6項未見明顯相關性。r OA組s-CTX-I、s-COMP、s-HA的含量分別為(270.40±52.01)pg/ml、(227.10±71.82)ng/ml、(14.11±5.53)ng/ml,顯著高于非r OA組,后者分別為(230.22±38.15)pg/ml、(176.44±40.66)ng/ml、(9.06±2.76)ng/ml(P0.05),其余6項生物標記物含量未見明顯差異。血清mi R-885-5p、mi R-let-7e、mi R-140-3p、mi R-28-3p及mi R-454的相對表達量隨著K-L分級逐漸增高,但與K-L分級及Outerbidge分級均無明顯相關性。[結論]血清CTX-I、COMP及HA的含量在OA診斷及病情評估中具有一定的意義,但易受其他代謝狀態(tài)、性別及年齡的影響。Micro RNAs能反映軟骨轉換狀態(tài),但對于OA的診斷價值仍需進一步明確。
[Abstract]:[objective] to evaluate the diagnostic value of tissue change biomarker and serum Micro RNAs, in patients with osteoarthritis. [methods] 44 cases of knee arthroscopy or initial arthroplasty were performed in our hospital from January 2015 to May 2015. Samples of serum, urine and joint fluid were collected before operation. The values of s-CTX-Inu-CTX-IIOS-COMPX-IIS-COMPX-COMPS-HAMPX-HA, and serum mi R-885-5pnmi R-let-7eguanmi R-140-3p, were determined. Mi R-28-3p and mi R-454. To evaluate the correlation between the amount of biomarkers and K-L grade and Outerbridge grade in different patients. According to K-L grade, the patients were divided into r OA group (imaging OA change group, radiological osteoarthritis,r OA) (K-L grade 鈮，

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