【摘要】：流體剪切力是生物體內(nèi)普遍存在的一種生物力學(xué)形式,是細(xì)胞微環(huán)境的重要組成部分,對(duì)細(xì)胞多種生物學(xué)行為有重要調(diào)節(jié)作用。該研究以微流控芯片技術(shù)為基礎(chǔ),建立了一種基于流阻原理能同時(shí)產(chǎn)生4個(gè)不同大小流體剪切力的微流控芯片平臺(tái),用以研究低流速的流體剪切力對(duì)大鼠原代軟骨細(xì)胞表型維持的影響。結(jié)果表明,流體剪切力可促進(jìn)軟骨細(xì)胞的表型維持。還加入了腫瘤壞死因子-α(TNF-α),考察流體剪切力和TNF-α共同作用對(duì)軟骨細(xì)胞表型的影響。結(jié)果表明,在剪切力和TNF-α共同作用下,軟骨細(xì)胞的Ⅱ型膠原和蛋白多糖表達(dá)明顯下調(diào)。該研究為軟骨組織工程和骨性關(guān)節(jié)炎的疾病研究提供有力的研究平臺(tái),為骨關(guān)節(jié)疾病治療和防治提供了理論依據(jù)。
[Abstract]:Fluid shear stress is a common biomechanical form in organisms and an important component of cell microenvironment. It plays an important role in regulating many biological behaviors of cells. Based on the technology of microfluidic chip, a microfluidic chip platform based on the principle of flow resistance can generate four different fluid shear forces at the same time. To study the effect of low velocity fluid shear stress on phenotypic maintenance of rat primary chondrocytes. The results showed that fluid shear stress promoted phenotypic maintenance of chondrocytes. Tumor necrosis factor- 偽 (TNF- 偽) was added to investigate the effects of fluid shear stress and TNF- 偽 on the phenotype of chondrocytes. The results showed that the expression of type 鈪，

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