【摘要】：目的研究迷走神經(jīng)電刺激(VNS)對(duì)腦外傷(TBI)昏迷大鼠的促醒效果及其機(jī)制。方法 168只健康Sprague-Dawley大鼠隨機(jī)分為空白組、TBI組、拮抗劑組和VNS組,每組42只�？瞻捉M不做任何處理,TBI組、VNS組、拮抗劑組撞擊法復(fù)制腦外傷模型,造模后昏迷至少30 min的大鼠入選。VNS組予VNS,拮抗劑組側(cè)腦室注射Orexin A受體1拮抗劑SB334867后加VNS,TBI組予假VNS。分別于干預(yù)后6 h、12 h、24 h觀察大鼠意識(shí)水平,應(yīng)用免疫組化、Western blotting檢測(cè)前額葉皮質(zhì)γ-氨基丁酸b1受體(GABAb1R)表達(dá)水平。結(jié)果最終空白組42只、TBI組11只、拮抗劑組13只、VNS組28只大鼠覺(jué)醒。Western blotting顯示,干預(yù)后12 h、24 h,GABAb1R蛋白表達(dá)TBI組拮抗劑組空白組VNS組(F60.412,P0.001)。免疫組化顯示,GABAb1R蛋白表達(dá)TBI組拮抗劑組VNS組空白組(H=15.121,P=0.002),但不同時(shí)間點(diǎn)無(wú)顯著性差異(H=3.028,P=0.220)。結(jié)論 VNS可促TBI昏迷大鼠覺(jué)醒,其促醒機(jī)制可能與Orexin A介導(dǎo)大鼠前額葉皮質(zhì)GABAb1R表達(dá)水平下調(diào)有關(guān)。
[Abstract]:Objective to study the effect and mechanism of vagal nerve stimulation (VNS) on brain injury in (TBI) coma rats. Methods 168 healthy Sprague-Dawley rats were randomly divided into control group, TBI group, antagonist group and VNS group with 42 rats in each group. TBI group, VNS group, antagonist group were used to make brain injury model, and the rats in the VNS group were selected to be in coma for at least 30 min. The VNS group was given VNS, antagonist group after injection of Orexin A receptor 1 antagonist SB334867 into the lateral ventricle and VNS, was added to the brain. TBI group was given false VNS. The level of consciousness was observed at 12 h and 24 h after intervention, and the expression of 緯 -aminobutyric acid b1 receptor (GABAb1R) in prefrontal cortex was detected by immunohistochemical, Western blotting. Results 42 rats in the blank group, 11 rats in the TBI group, 13 rats in the antagonist group and 28 rats in the VNS group had awakened. Western blotting. The expression of GABAb1R protein in the control group (F60.412P0.001) was detected 24 hours after the intervention. The expression of GABAb1R protein in the TBI group was significantly higher than that in the control group (F60.412P0.001). Immunohistochemical staining showed that GABAb1R protein was expressed in TBI antagonist group, VNS group was blank group (Hn15.121 P0. 002), but there was no significant difference at different time points (H0. 028, P0. 220). Conclusion VNS can promote the arousal of TBI coma rats, and its mechanism may be related to the down-regulation of GABAb1R expression in prefrontal cortex induced by Orexin A.
【作者單位】： 南昌大學(xué)第一附屬醫(yī)院康復(fù)醫(yī)學(xué)科;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81260295)
【分類號(hào)】：R651.15

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 陶玉;尹海兵;陳旭東;宗桂娟;劉玉山;何松;;UBE2C基因在食管鱗癌中的表達(dá)及其臨床意義[J];江蘇醫(yī)藥;2016年16期

2 Zhen Feng;Qing Du;;Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injuryinduced coma: orexin-A-mediated N-methyl-Daspartate receptor subunit NR1 upregulation[J];Neural Regeneration Research;2016年06期

3 董曉陽(yáng);馮珍;;迷走神經(jīng)電刺激對(duì)腦外傷昏迷大鼠前額葉皮質(zhì)5-羥色胺2A受體表達(dá)的影響[J];中國(guó)康復(fù)理論與實(shí)踐;2016年04期

4 袁蓮芳;陳果;侯麗;劉利萍;賈宏閣;師建國(guó);;γ-氨基丁酸B受體與應(yīng)激性防御反應(yīng)研究現(xiàn)狀[J];中華實(shí)用診斷與治療雜志;2015年06期

5 Zhen Feng;Ying-jun Zhong;Liang Wang;Tian-qi Wei;;Resuscitation therapy for traumatic brain injuryinduced coma in rats:mechanisms of median nerve electrical stimulation[J];Neural Regeneration Research;2015年04期

6 Long Zhou;Jinhuang Lin;Junming Lin;Guoju Kui;Jianhua Zhang;Yigang Yu;;Neuroprotective effects of vagus nerve stimulation on traumatic brain injury[J];Neural Regeneration Research;2014年17期

7 宋璐;劉愛(ài)華;;迷走神經(jīng)刺激術(shù)在非癲癇領(lǐng)域的應(yīng)用進(jìn)展[J];中華臨床醫(yī)師雜志(電子版);2012年17期

8 Dietmar Benke;Khaled Zemoura;Patrick J Maier;;Modulation of cell surface GABA B receptors by desensitization,trafficking and regulated degradation[J];World Journal of Biological Chemistry;2012年04期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 獨(dú)家能;劉聰;郝旭亮;倪艷;許雯雯;季海霞;;生理性失眠發(fā)病機(jī)制的研究進(jìn)展[J];中國(guó)醫(yī)藥導(dǎo)報(bào);2017年29期

2 廖誠(chéng)誠(chéng);馮珍;黃菲菲;陳琴;;迷走神經(jīng)電刺激對(duì)腦外傷昏迷大鼠意識(shí)及前額葉皮質(zhì)γ-氨基丁酸b1受體表達(dá)的影響[J];中國(guó)康復(fù)理論與實(shí)踐;2017年09期

3 劉寶虎;郭彤彤;江繼鵬;陳旭義;邊科砜;張賽;;β-七葉皂苷鈉對(duì)顱腦創(chuàng)傷大鼠腦水腫的保護(hù)作用[J];天津醫(yī)藥;2017年09期

4 董曉陽(yáng);劉丹;黃菲菲;馮珍;;迷走神經(jīng)電刺激對(duì)腦外傷后昏迷大鼠前額葉皮質(zhì)和下丘腦Orexin-A及其受體OX1R表達(dá)變化的影響[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2017年07期

5 陳琴;杜青;馮珍;;MNES對(duì)腦損傷后昏迷大鼠PFC去甲腎上腺素α_1受體表達(dá)的影響[J];重慶醫(yī)學(xué);2017年18期

6 廖誠(chéng)誠(chéng);馮珍;;正中神經(jīng)電刺激用于昏迷促醒的機(jī)制研究進(jìn)展[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2017年05期

7 杜青;馮珍;;正中神經(jīng)電刺激腦外傷昏迷大鼠前額葉皮質(zhì)5-HT 2A受體表達(dá)的實(shí)驗(yàn)研究[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2017年03期

8 古麗;阿迪力江·賽買(mǎi)提;阿地力·莫明;;GABRB3基因單核苷酸多態(tài)性與新疆地區(qū)維吾爾人群非綜合征性唇腭裂的相關(guān)性[J];中華實(shí)用診斷與治療雜志;2017年02期

9 陳琴;黃菲菲;董曉陽(yáng);馮珍;;迷走神經(jīng)電刺激對(duì)腦外傷后昏迷大鼠前額葉皮質(zhì)去甲腎上腺素α1受體表達(dá)變化的影響[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2017年01期

10 楊初燕;王亮;馮珍;邵秀芹;;正中神經(jīng)電刺激對(duì)腦外傷后昏迷患者促醒作用的臨床及機(jī)制研究[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2016年11期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊岑;冉明梓;歐陽(yáng)鵬榮;王百忍;董海龍;;五羥色胺在睡眠-覺(jué)醒中作用[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2015年11期

2 Zhen Feng;Ying-jun Zhong;Liang Wang;Tian-qi Wei;;Resuscitation therapy for traumatic brain injuryinduced coma in rats:mechanisms of median nerve electrical stimulation[J];Neural Regeneration Research;2015年04期

3 魏天祺;馮珍;;迷走神經(jīng)電刺激臨床應(yīng)用及機(jī)制研究進(jìn)展[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2015年02期

4 Richard Justin Garling;Lora Talley Watts;Shane Sprague;Lauren Fletcher;David F.Jimenez;Murat Digicaylioglu;;Does progesterone show neuroprotective effects on traumatic brain injury through increasing phosphorylation of Akt in the hippocampus?[J];Neural Regeneration Research;2014年21期

5 Shruti V.Kabadi;Alan I.Faden;;Selective CDK inhibitors:promising candidates for future clinical traumatic brain injury trials[J];Neural Regeneration Research;2014年17期

6 王成;;芬戈莫德聯(lián)合骨髓間充質(zhì)干細(xì)胞移植腦創(chuàng)傷大鼠記憶功能的變化[J];中國(guó)組織工程研究;2014年28期

7 張洪;章軍建;;血管性認(rèn)知功能障礙防治進(jìn)展[J];中華實(shí)用診斷與治療雜志;2013年11期

8 石玉鋒;虞燕琴;;谷氨酸在睡眠和覺(jué)醒中的作用研究進(jìn)展[J];浙江大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2013年05期

9 段樂(lè);白利群;田苗苗;張兵;;炎癥反應(yīng)在腦缺血中作用機(jī)制研究進(jìn)展[J];中華實(shí)用診斷與治療雜志;2013年09期

10 李強(qiáng);陳寶友;李建國(guó);;神經(jīng)核團(tuán)毀損術(shù)與電刺激術(shù)治療帕金森病療效觀察[J];中華實(shí)用診斷與治療雜志;2013年09期

，

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