【摘要】：背景:膝關節(jié)軟骨損傷后修復較困難。有研究發(fā)現(xiàn)將關節(jié)軟骨細胞和滑膜間充質(zhì)干細胞混合培養(yǎng)接種于殼聚糖支架材料中,均可以形成軟骨基質(zhì)。目的:觀察滑膜間充質(zhì)干細胞與軟骨細胞與殼聚糖/Ⅰ型膠原復合支架材料對膝關節(jié)軟骨缺損的修復情況。方法:將大鼠膝關節(jié)滑膜間充質(zhì)干細胞及軟骨細胞按1∶2比例共培養(yǎng)于殼聚糖/Ⅰ型膠原復合支架材料中,將此復合材料移植于膝關節(jié)軟骨缺損模型大鼠膝關節(jié)缺損處進行修復。結果與結論:(1)組織學變化:蘇木精-伊紅染色、甲苯胺藍染色及番紅O染色顯示,植入后4,8,12周,大鼠膝關節(jié)軟骨細胞逐漸增多;(2)軟骨樣組織標記物Ⅱ型膠原表達:免疫組織化學染色顯示,移植后4,8,12周,大鼠膝關節(jié)軟骨組織細胞及細胞基質(zhì)可見Ⅱ型膠原陽性表達。(3)結果證實,滑膜間充質(zhì)干細胞與軟骨細胞混合培養(yǎng)于殼聚糖/Ⅰ型膠原復合支架材料中,在體內(nèi)環(huán)境下能夠形成軟骨樣組織,有利于損傷膝關節(jié)軟骨缺損的修復。
[Abstract]:Background: repair of articular cartilage is difficult after injury. It was found that chondrocytes and synovial mesenchymal stem cells could be cultured in chitosan scaffold to form cartilage matrix. Aim: to observe the repair of cartilage defect of knee joint with synovial mesenchymal stem cells chondrocytes and chitosan / type I collagen composite scaffold. Methods: rat synovial mesenchymal stem cells and chondrocytes were co-cultured in chitosan / type I collagen composite scaffold at 1:2 ratio. The composite material was transplanted to the knee joint defect of the rat model of knee cartilage defect for repair. Results and conclusion: (1) histological changes: hematoxylin eosin staining toluidine blue staining and Phanerin O staining showed that the number of chondrocytes in the knee joint of rats gradually increased at 812 weeks after implantation. (2) expression of type 鈪，

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