右美托咪定預(yù)處理對(duì)小鼠腎缺血再灌注后肝腎組織炎性因子和氧化應(yīng)激的影響
發(fā)布時(shí)間：2018-11-05 20:51
【摘要】：目的探討右美托咪定預(yù)處理對(duì)小鼠缺血再灌注損傷后肝腎功能的影響。方法選取8周齡C57BL/6雄性小鼠24只,隨機(jī)分為3組(n=8):正常對(duì)照組(Sham組)、缺血再灌注組(IR組)和右美托咪定處理組(IR+Dex組)。IR+Dex組小鼠于造模前25 min腹腔注射右美托咪定(50μg/kg),Sham組和IR組于造模前25min腹腔注射同等量的生理鹽水,小鼠腎缺血再灌注模型建立成功后于再灌注24 h處死取材。分別取小鼠血清檢測(cè)血肌酐(Scr)、尿素氮(BUN)、丙氨酸氨基轉(zhuǎn)移酶(ALT)和天門(mén)氨酸氨基轉(zhuǎn)移酶(AST)等腎功能及肝功能指標(biāo),取腎臟和肝臟組織勻漿液檢測(cè)超氧化物歧化酶(SOD)、丙二醛(MDA)、腫瘤壞死因子-α(TNF-α)、白細(xì)胞介素-6(IL-6)、單核細(xì)胞趨化因子-1(MCP-1)和白細(xì)胞介素-10(IL-10)等指標(biāo)。結(jié)果與Sham組比較,IR組小鼠血清中Scr、BUN、ALT和AST等指標(biāo)含量明顯升高(P0.05);IR組小鼠腎臟、肝臟組織勻漿液中SOD明顯降低(P0.05),MDA、TNF-α、IL-6、MCP-1和IL-10明顯升高(P0.05);與IR組比較,IR+Dex組小鼠血清中Scr、BUN、ALT和AST等指標(biāo)含量明顯降低(P0.05);IR+Dex組小鼠腎臟、肝臟組織勻漿液中SOD和IL-10明顯升高(P0.05),MDA、TNF-α、IL-6和MCP-1明顯降低(P0.05)。結(jié)論右美托咪定能明顯減輕小鼠腎缺血再灌注致腎、肝損傷的程度,其機(jī)制可能與提高小鼠缺血再灌注后腎、肝的抗氧化能力,抑制氧自由基堆積有關(guān)。
[Abstract]:Objective to investigate the effect of dexmetomidine preconditioning on liver and kidney function after ischemia reperfusion injury in mice. Methods Twenty-four 8-week-old C57BL/6 mice were randomly divided into 3 groups: normal control group (Sham group). The mice in the ischemic reperfusion group (IR group) and dexmetomidine group (IR Dex group) were intraperitoneally injected with dexmetomidine (50 渭 g/kg), Sham group and IR group) by intraperitoneal injection of dexmetomidine (50 渭 g/kg), Sham group and IR group) at 25 min before the establishment of the model, and the same amount of normal saline was injected intraperitoneally to the). IR Dex group before modeling. The kidney ischemia reperfusion model of mice was successfully established and killed 24 h after reperfusion. Serum levels of serum creatinine, (Scr), urea nitrogen, (BUN), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured for renal function and liver function. Superoxide dismutase (SOD), malondialdehyde (MDA),) tumor necrosis factor- 偽 (TNF- 偽) and interleukin-6 (IL-6) were detected in kidney and liver tissue homogenate. Monocyte chemokine-1 (MCP-1) and interleukin-10 (IL-10). Results compared with Sham group, the contents of Scr,BUN,ALT and AST in serum of IR group were significantly higher than those of Sham group (P0.05). In IR group, SOD in kidney and liver homogenate significantly decreased (P0.05), MDA,TNF- 偽, IL-6,MCP-1 and IL-10 increased significantly (P0.05). Compared with IR group, the contents of Scr,BUN,ALT and AST in serum of, IR Dex group were significantly lower than those of, IR Dex group (P0.05). In IR Dex group, SOD and IL-10 in kidney and liver tissue homogenate increased significantly (P0.05), MDA,TNF- 偽, IL-6 and MCP-1 decreased significantly (P0.05). Conclusion dexmetomidine can significantly reduce the degree of renal and liver injury induced by renal ischemia reperfusion in mice. The mechanism may be related to the increase of the antioxidant capacity of kidney and liver and the inhibition of oxygen free radical accumulation after ischemia reperfusion in mice.
【作者單位】： 河南大學(xué)淮河醫(yī)院麻醉科;錦州醫(yī)科大學(xué)研究生院;
【基金】：河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃項(xiàng)目(編號(hào):201404029) 開(kāi)封市科技發(fā)展計(jì)劃項(xiàng)目(編號(hào):1603065)
【分類(lèi)號(hào)】：R614
本文編號(hào)：2313420
[Abstract]:Objective to investigate the effect of dexmetomidine preconditioning on liver and kidney function after ischemia reperfusion injury in mice. Methods Twenty-four 8-week-old C57BL/6 mice were randomly divided into 3 groups: normal control group (Sham group). The mice in the ischemic reperfusion group (IR group) and dexmetomidine group (IR Dex group) were intraperitoneally injected with dexmetomidine (50 渭 g/kg), Sham group and IR group) by intraperitoneal injection of dexmetomidine (50 渭 g/kg), Sham group and IR group) at 25 min before the establishment of the model, and the same amount of normal saline was injected intraperitoneally to the). IR Dex group before modeling. The kidney ischemia reperfusion model of mice was successfully established and killed 24 h after reperfusion. Serum levels of serum creatinine, (Scr), urea nitrogen, (BUN), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured for renal function and liver function. Superoxide dismutase (SOD), malondialdehyde (MDA),) tumor necrosis factor- 偽 (TNF- 偽) and interleukin-6 (IL-6) were detected in kidney and liver tissue homogenate. Monocyte chemokine-1 (MCP-1) and interleukin-10 (IL-10). Results compared with Sham group, the contents of Scr,BUN,ALT and AST in serum of IR group were significantly higher than those of Sham group (P0.05). In IR group, SOD in kidney and liver homogenate significantly decreased (P0.05), MDA,TNF- 偽, IL-6,MCP-1 and IL-10 increased significantly (P0.05). Compared with IR group, the contents of Scr,BUN,ALT and AST in serum of, IR Dex group were significantly lower than those of, IR Dex group (P0.05). In IR Dex group, SOD and IL-10 in kidney and liver tissue homogenate increased significantly (P0.05), MDA,TNF- 偽, IL-6 and MCP-1 decreased significantly (P0.05). Conclusion dexmetomidine can significantly reduce the degree of renal and liver injury induced by renal ischemia reperfusion in mice. The mechanism may be related to the increase of the antioxidant capacity of kidney and liver and the inhibition of oxygen free radical accumulation after ischemia reperfusion in mice.
【作者單位】： 河南大學(xué)淮河醫(yī)院麻醉科;錦州醫(yī)科大學(xué)研究生院;
【基金】：河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃項(xiàng)目(編號(hào):201404029) 開(kāi)封市科技發(fā)展計(jì)劃項(xiàng)目(編號(hào):1603065)
【分類(lèi)號(hào)】：R614
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