【摘要】：為研究體外循環(huán)機(jī)持續(xù)灌注、間斷壓力灌注、單純低溫保存犬離體肺對(duì)支氣管肺泡灌洗液中中性粒細(xì)胞凋亡變化以及對(duì)肺泡巨噬細(xì)胞吞噬凋亡中性粒細(xì)胞的影響,探索最佳的離體肺保存方式,將30只Mongrel犬隨機(jī)分成3組,為體外循環(huán)組、壓力灌注組和低溫保存組,每組10只。在保持機(jī)械通氣的條件下,完整摘取雙肺,3種不同方式保存離體肺,并按時(shí)間點(diǎn)留取支氣管肺泡灌洗液標(biāo)本。流式細(xì)胞儀檢測(cè)犬離體肺支氣管肺泡灌洗液中中性粒細(xì)胞凋亡,免疫組織化學(xué)法檢測(cè)犬離體肺肺泡巨噬細(xì)胞對(duì)凋亡中性粒細(xì)胞吞噬。隨著犬肺離體時(shí)間的推移,各實(shí)驗(yàn)組離體肺支氣管肺泡灌洗液中凋亡的中性粒細(xì)胞明顯減少,在每個(gè)時(shí)間點(diǎn)上,體外循環(huán)組中性粒細(xì)胞的凋亡最多,壓力灌注組中次之,低溫保存組中最少。各實(shí)驗(yàn)組吞噬凋亡中性粒細(xì)胞的巨噬細(xì)胞隨著離體時(shí)間的延長(zhǎng)呈逐漸減少的趨勢(shì),在肺離體各時(shí)間點(diǎn)上,體外循環(huán)組中巨噬細(xì)胞對(duì)凋亡中性粒細(xì)胞的吞噬清除優(yōu)于間斷壓力灌注組和單純低溫保存組。采用體外循環(huán)機(jī)持續(xù)灌注犬離體肺,能增加中性粒細(xì)胞凋亡,促使吞噬細(xì)胞吞噬凋亡的中性粒細(xì)胞,避免發(fā)生凋亡延遲,從而減輕離體肺組織的缺血再灌注損傷,具有較好的肺保護(hù)作用。
[Abstract]:In order to study the effects of continuous perfusion of cardiopulmonary bypass (CPB), intermittent pressure perfusion and cryopreservation on the apoptosis of neutrophils in bronchoalveolar lavage fluid (BALF) and the phagocytosis of alveolar macrophages (AMs) in dogs. To explore the best method of lung preservation in vitro, 30 Mongrel dogs were randomly divided into three groups: cardiopulmonary bypass group, pressure perfusion group and hypothermia preservation group with 10 dogs in each group. Under the condition of maintaining mechanical ventilation, the lungs were removed completely, the isolated lungs were preserved in three different ways, and the bronchoalveolar lavage fluid specimens were collected according to the time points. The apoptosis of neutrophils in the bronchoalveolar lavage fluid was detected by flow cytometry, and the phagocytosis of apoptotic neutrophils by alveolar macrophages was detected by immunohistochemistry. The neutrophil apoptosis in bronchoalveolar lavage fluid (BALF) was significantly decreased in each experimental group with the passage of time in vitro. At each time point, neutrophil apoptosis was the most in cardiopulmonary bypass group, followed by pressure perfusion group. The lowest in cryopreservation group. The macrophages phagocytosis of apoptotic neutrophils in each experimental group showed a decreasing trend with the prolongation of the time in vitro. The phagocytosis of apoptotic neutrophils in cardiopulmonary bypass group was better than that in intermittent pressure perfusion group and cryopreservation group. Continuous perfusion of isolated canine lung by extracorporeal circulation can increase neutrophil apoptosis, promote phagocytosis of apoptotic neutrophil, avoid delayed apoptosis, and reduce ischemia-reperfusion injury in isolated lung tissue. It has better lung protective effect.
【作者單位】： 昆明醫(yī)科大學(xué)第一附屬醫(yī)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81060010)資助
【分類(lèi)號(hào)】：R655.3

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