【摘要】：目的：急性脊髓損傷(acute spinal cord injury, ASCI)是一種嚴(yán)重的神經(jīng)系統(tǒng)創(chuàng)傷。本實驗建立急性脊髓損傷大鼠模型,觀察其腦脊液差異蛋白譜,從微觀分子水平研究急性脊髓損傷后繼發(fā)性損傷的機制及有效治療方法。方法：建立SD大鼠急性脊髓損傷模型,取腦脊液應(yīng)用同位素標(biāo)記相對和絕對定量(iTRAQ)技術(shù)鑒定SD大鼠急性脊髓損傷后腦脊液的差異蛋白質(zhì)。結(jié)果：共鑒定蛋白數(shù)722個,差異表達蛋白107個：下調(diào)的差異蛋白有63個,上調(diào)的差異蛋白數(shù)是44個。其中相關(guān)神經(jīng)再生的差異蛋白19個：上調(diào)14個,下調(diào)5個；調(diào)節(jié)神經(jīng)再生的差異蛋白7個。結(jié)論：實驗中檢測到的多種差異蛋白及表達明顯的神經(jīng)再生因子可能作為急性脊髓損傷的生物標(biāo)記物或為臨床管理監(jiān)測急性脊髓損傷的損傷進程、靶向治療及評估療效的強有力證據(jù)。
[Abstract]:Objective: acute spinal cord injury (acute spinal cord injury, ASCI) is a severe nervous system injury. In this study, the rat model of acute spinal cord injury (sci) was established, and the differential protein spectrum of CSF was observed, and the mechanism and effective treatment of secondary injury after acute spinal cord injury (Asci) were studied at micromolecular level. Methods: the acute spinal cord injury (sci) model of SD rats was established. The differential proteins in CSF of SD rats after acute spinal cord injury were identified by using isotope labeled relative and absolute quantitative (iTRAQ) techniques. Results: 722 proteins were identified, 107 differentially expressed proteins were identified, 63 differentially expressed proteins were down-regulated and 44 differentially expressed proteins were upregulated. Among the 19 differentially expressed proteins, 14 were up-regulated, 5 were down-regulated, and 7 were related to nerve regeneration. Conclusion: the differentially expressed proteins and neural regeneration factors may be used as biomarkers of acute spinal cord injury or as clinical management to monitor the process of acute spinal cord injury. Strong evidence of targeted therapy and evaluation of efficacy.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R651.2
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本文編號：2306029