【摘要】：目的探討利用數(shù)字化技術(shù)建立大鼠跨區(qū)穿支皮瓣微小血管模型的可行性及應(yīng)用價值。方法取8周齡SPF級雌性SD大鼠20只,體質(zhì)量280~300 g,于背部制備面積約10 cm×3 cm的跨區(qū)穿支皮瓣,然后原位縫合。術(shù)后3、7 d各取10只大鼠,大體觀察皮瓣壞死情況并測算皮瓣壞死面積百分比;然后采用明膠-氧化鉛溶液行血管灌注,取材行micro-CT掃描并血管三維重建,采用Matlable7.0軟件測算血管總長度及容積。結(jié)果術(shù)后3 d皮瓣壞死面積百分比為19.08%±3.64%,顯著低于術(shù)后7 d的39.76%±3.76%(t=10.361,P=0.029)。micro-CT血管三維重建能清晰顯示皮瓣微小血管形態(tài)變化;術(shù)后3 d皮瓣血管容積為(1 240.23±89.71)mm~3、血管總長度為(245.94±29.38)mm,與術(shù)后7 d的(1 036.96±88.97)mm~3、(143.20±30.28)mm比較,差異均有統(tǒng)計學(xué)意義(t=5.088,P=0.000;t=7.701,P=0.000)。結(jié)論數(shù)字化技術(shù)能夠可視化觀察、客觀評估大鼠跨區(qū)穿支皮瓣微小血管形態(tài)變化,為皮瓣血管模型研究提供技術(shù)支持。
[Abstract]:Objective to explore the feasibility and application value of digital technique in the establishment of microvascular model of transregional perforator flap in rats. Methods Twenty 8-week-old female SD rats of SPF grade, weighing 280 ~ 300g, were used to prepare a cross-region perforator flap with an area of about 10 cm 脳 3 cm on the back, and then sutured in situ. 10 rats were taken from each group on the 7th day after operation. The necrosis of the flap was observed and the percentage of the necrotic area of the flap was calculated. Then the blood vessels were perfused with gelatin-lead oxide solution, micro-CT scanning and 3D vascular reconstruction were performed. The total length and volume of blood vessels were measured by Matlable7.0 software. Results the percentage of necrosis area was 19.08% 鹵3.64 on the 3rd day after operation, which was significantly lower than that on the 7th day after operation (39.76% 鹵3.76%). Three-dimensional micro-CT vascular reconstruction could clearly show the morphological changes of the small vessels of the flap. The vascular volume of the flap 3 days after operation was (1 240.23 鹵89.71) mm~3, (245.94 鹵29.38) mm, and (1 036.96 鹵88.97) mm~3, (143.20 鹵30.28) mm on the 7th day after operation. P0. 000; T = 7.701g, P = 0.000). Conclusion the digital technique can be used to visualize and evaluate the morphological changes of microvessels in transregional perforating flaps of rats, and to provide technical support for the study of vascular models of skin flaps.
【作者單位】： 解放軍昆明總醫(yī)院骨科;中國人民解放軍77251部隊醫(yī)院外科;
【基金】：全軍后勤科研計劃面上項目(CCD14J003) 全軍后勤科研計劃重大專項(AWS14C003)~~
【分類號】：R-332;R622

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